Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Kunstmann‐Olsen, Casper; Hanczyc, Martin M.; Hoyland, James; Rasmussen, Steen; Rubahn, Horst‐Günter

doi:10.1016/j.snb.2016.01.120

Cited by 38 publications

(29 citation statements)

References 21 publications

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“…The optical droplet detection method involves the integration of a light source and detection unit into a microfluidic chip to measure droplet size, size distribution, shape, frequency, velocity and composition. [13][14][15][16][17][18][19][20][21][22] De Saint Vincent et al presented a realtime droplet detection system to measure droplet length, velocity and frequency using two photodiodes. 18 Although recent optical systems are usually on-chip and do not require bench-top hardware, they have low reproducibility due to the stringent alignment procedure between the light source and photodiodes.…”

Section: Introductionmentioning

confidence: 99%

Real-time impedimetric droplet measurement (iDM)

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Real-time impedimetric droplet measurement (iDM)

et al. 2019

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“…Microdroplet manipulation is significant in some situations, for example, self‐organization of materials, [ 1 ] droplet transport, [ 2–5 ] droplet rebounding, [ 6,7 ] spontaneous droplet trampolining, [ 8 ] liquid pattern, [ 9–11 ] droplet‐based sensor, [ 12 ] anti‐icing, [ 13 ] and water harvesting, [ 14,15 ] droplet self‐division, [ 16–18 ] droplet controlling in microfluidics, [ 19–22 ] droplet splitting, [ 23 ] multi‐phase fluid interactions, [ 24 ] etc. For manipulating the microdroplet, some external actions (e.g., electronic, [ 19,20 ] magnetic, [ 21 ] photo, [ 25 ] etc.)…”

Section: Figurementioning

confidence: 99%

Droplet Manipulation: Magically Cut Apart Microdroplet by Smart Nanofibrils Wire

Zhang

Pei

et al. 2020

Adv Materials Inter

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only via magical action of an ethanolwetted nanofibrils wire (ENW). The ENW can be designed and fabricated via electronspinning and liquid-infused processes. We reveal that ENW can smartly cut the spreading water droplets via manipulation on a surface, without other external energy. We demonstrate that spreading water droplets with volume of 5-25 µL can be accurately cut apart into various proportions (e.g., 1/9-1/1) by manipulating ENW, and the minimum proportion would be 1.7 µL in volume, without loss. Furthermore, we demonstrate that the radial-crossed 3-5 ENWs can cut apart a spreading droplet effectively into split droplets (SDs) pattern in one step. Especially, we demonstrate that the SDs can be manipulated to move to target regions (e.g., microreactors) via actions of ENW. These findings offer insights into design of novel materials that would be used to manipulate the various liquid microdroplets on a superhydrophilic surface, where droplet movement follows guide of magical ENW, without other external energy, which would be extended into microfluidics, microreactors, etc.The magical ENW can be designed by using electrospinning PAN (poly-acrylonitrile) nanofibers surrounding a 100 µm nylon-main fiber, along with a hydrothermal reaction for a hierarchical high-porosity interlaced network and ethanol-infused process (the details are presented in Figure S1, Supporting Information). The ENW is composed of nanofibrils that are covered with nanopetals (Figure 1a, the inset). The nanopetals makes rough nanoporous surface of nanofibrils, which can be a high capillary to improve the wetting (a typical case can be seen in Figure S2, Supporting Information). [25] Accordingly, a magical ENW can be achieved into robustness after ethanol-infused inside nanoporous surface of ENW. Finally, it is expected that ENW can be used to cut apart a spreading microdroplet on a superhydrophilic surface, that is, a spreading microdroplet would be cut apart into split droplets (e.g., SD-1, and SD-2, respectively, Figure 1a) by means of unique vapor-liquid repellency effect. To best of our knowledge, such droplet splitting behavior is not reported so far.It is found that when such an ENW horizontally approaches to the upper of a spreading droplet and achieves the stronger concave of the spreading droplet, and finally cut apart intoThe ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/admi.202000161.Microdroplet manipulation is significant in some situations, for example, self-organization of materials, [1] droplet transport, [2][3][4][5] droplet rebounding, [6,7] spontaneous droplet trampolining, [8] liquid pattern, [9][10][11] droplet-based sensor, [12] anti-icing, [13] and water harvesting, [14,15] droplet self-division, [16][17][18] droplet controlling in microfluidics, [19][20][21][22] droplet splitting, [23] multi-phase fluid interactions, [24] etc. For manipulating the microdroplet, some external actions (e.g., electronic, [19,20] magnetic, [21] photo, [25] etc.) are usual...

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“…Soft lithography is a low-cost and useful method for the fabrication of PDMS devices [2,3] and allows rapid prototyping of microfluidic devices [4,5]. Therefore, various systems such as microelectronics and micro-electromechanical systems (MEMS) [6][7][8][9][10] fabricated by PDMS have been reported. More importantly, PDMS has many properties including elasticity and robustness that are desirable for using in an on-chip actuator system.…”

Section: Introductionmentioning

confidence: 99%

Property Investigation of Replaceable PDMS Membrane as an Actuator in Microfluidic Device

et al. 2018

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This paper investigates the basic deflection properties of polydimethylsiloxane (PDMS) membrane as an actuator component in a microfluidic device. Polydimethylsiloxane membrane is a widely used structure in various applications in microfluidics. Most of the applications using PDMS membrane as actuators are pumps, valves, microlenses, and cell stimulators. In these applications, PDMS membranes are deflected to function by applied pressure. However, based on our literature survey, correlations between thickness, applied air pressure, and the deflection properties of replaceable PDMS membrane have not been theoretically and experimentally investigated yet. In this paper, we first conducted a simulation to analyze the relationship between deflection of the replaceable PDMS membrane and applied pressure. Then we verified the deflection of the PDMS membrane in different experimental conditions. Finally, we demonstrated that the PDMS membrane functioned as a valve actuator in a cell-capturing device as one application. We expect this study would work as an important reference for research investigations that use PDMS membrane as an actuator.

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Uniform droplet splitting and detection using Lab-on-Chip flow cytometry on a microfluidic PDMS device

Cited by 38 publications

References 21 publications

Real-time impedimetric droplet measurement (iDM)

Real-time impedimetric droplet measurement (iDM)

Droplet Manipulation: Magically Cut Apart Microdroplet by Smart Nanofibrils Wire

Property Investigation of Replaceable PDMS Membrane as an Actuator in Microfluidic Device

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