Uniaxial Diffusional Narrowing of NMR Lineshapes for Membrane Proteins Reconstituted in Magnetically Aligned Bicelles and Macrodiscs

Peptoid-based macrodiscs of variable lipid composition for structural studies of membrane proteins by oriented-sample solid-state NMR

“…3 B, 2D SLF NMR spectra show a systematic increase in the degree of magnetic ordering for the aligned macrodiscs vs. DMPC/DHPC bicelles as given by an increased range for the maximum observed dipolar couplings (>4 kHz). Such an improvement in the order parameter is clearly seen by comparing 14-mer belt peptide ( Park et al, 2011 , Tesch et al, 2018 ) macrodiscs (red contours) with DMPC/DHPC bicelles (green contours) (S 0 = 0.83 vs. 0.80), which increases to 0.85 in the case of DMPC-only 15-mer peptoid-based macrodiscs (blue peaks), and even further for the sample containing 15 % of DMPG (S 0 = 0.9; magenta peaks). Fig.…”

“…The spectrum of Pf1 in 14-mer peptide discs were taken from ref. ( Tesch et al, 2018 ) for comparison, having a 10 % w/v lipid concentration and a peptide-to-lipid ratio of 1:13.3.…”