The checklist is based on 137 references and it includes data on the distribution of 220 taxa of lichenicolous fungi in Ukraine. Four species are based on dubious records. The Crimea (80 species) and Carpathians Mts (64 species) have highest observed species diversity of the lichenicolous fungi. Among plain territories, most species-rich regions are Kherson (64 species) and Mykolaiv (37 species), and both regions were intensively surveyed for lichenicolous fungi.