Unexplored Genetic Diversity of Multidrug- and Extremely Drug-Resistant <i>Acinetobacter baumannii</i> Isolates from Tertiary Hospitals in Honduras

López-Leal, Gamaliel; Zuniga-Moya, Julio Cesar; Castro-Jaimes, Semiramis; Graña-Miraglia, Lucía; Pérez-Oseguera, Ángeles; Reyes-García, Hilda Sarahi; Gough-Coto, Simmons; Pavón-Madrid, René; Bejarano, Suyapa; Ferrera, Annabelle; Castillo-Ramírez, Santiago; Cevallos, Miguel A.

doi:10.1089/mdr.2018.0311

Cited by 16 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). According to the PubMLST database, several studies have reported isolates from this ST in Canada (24), Mexico and Honduras (19,23), and even Colombia (25). Of note, ST758 and ST1091 correspond to ST156 under the MLST Pasteur scheme.…”

Section: Resultsmentioning

confidence: 99%

“…To solve this, we recently carried out a few studies. In the first study, we conducted WGS of 8 isolates from Mexico's National Institute of Oncology (INCAN) collected from 2011 to 2013 (19), whereas in the second study, we sequenced 16 isolates from 5 hospitals in Honduras (23). Here, to extend those two previous data sets and to put together the most extensive data set considering isolates from North America (Canada and Mexico) and Central America (Honduras), we sequenced another 10 isolates from the National Institute of Rehabilitation (INR), which is a tertiary hospital in Mexico City, Mexico.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Graña-Miraglia

Evans

López‐Jácome

et al. 2020

mSphere

Self Cite

View full text Add to dashboard Cite

Over the last few decades, carbapenemase-producing Acinetobacter baumannii has become a major cause of nosocomial infections all over the world. However, the genome identity of lineages of this species in Latin America has not been studied as much as in developed countries. Here, through a population genomics approach considering the whole genomes of 148 isolates (almost 40 from Mexico and Honduras), we describe the recent emergence of the lineage sequence type 758 (ST758), which belongs to the international clone V and has spread out to Canada, Mexico, Honduras, and Colombia. Notably, this lineage was found to coexist with other A. baumannii lineages in hospitals in Mexico and Honduras. Isolates from this lineage show considerable variation in antibiotic resistance profiles, but most of them are resistant to carbapenems. Moreover, we found a variety of acquired oxacillinase (OXA) families within this lineage and tracked the very recent inception, and subsequent horizontal transmission, of the OXA-239 carbapenemase. This work highlights the urgent need to investigate recently emerged lineages of this species in Latin America and elsewhere, as these might harbor novel antibiotic resistance genes. IMPORTANCE A. baumannii is a major cause of nosocomial infections all over the world. Although many isolates from developed countries have been studied in terms of their genome sequence, isolates from Latin America have been much less studied. In this study, using a population genomics approach considering the whole genomes of 148 isolates, we describe the recent emergence of the lineage ST758 endemic to Latin America and the inception of the OXA-239 carbapenemase. Our study highlights the urgent need to investigate recently emerged lineages of this species in Latin America and elsewhere, as these might harbor novel antibiotic resistance genes.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Graña-Miraglia

Evans

López‐Jácome

et al. 2020

mSphere

Self Cite

View full text Add to dashboard Cite

show abstract

“…The MLST758 is an understudied lineage that does not belong to the international clones I and II; it has spread to Canada, Mexico, Honduras, and Colombia. This lineage represents a source of genetic diversity of MDR and XDR isolates that have not been studied [ 20 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

High mortality in an outbreak of multidrug resistant Acinetobacter baumannii infection introduced to an oncological hospital by a patient transferred from a general hospital

et al. 2020

Self Cite

View full text Add to dashboard Cite

Objective To describe the clinical features, outcomes, and molecular epidemiology of an outbreak of multidrug resistant (MDR) A. baumannii. Methods We performed a retrospective analysis of all MDR A. baumannii isolates recovered during an outbreak from 2011 to 2015 in a tertiary care cancer hospital. Cases were classified as colonized or infected. We determined sequence types following the Bartual scheme and plasmid profiles. Results There were 106 strains of A. baumannii isolated during the study period. Sixty-six (62.3%) were considered as infection and 40 (37.7%) as colonization. The index case, identified by molecular epidemiology, was a patient with a drain transferred from a hospital outside Mexico City. Ninety-eight additional cases had the same MultiLocus Sequence Typing (MLST) 758, of which 94 also had the same plasmid profile, two had an extra plasmid, and two had a different plasmid. The remaining seven isolates belonged to different MLSTs. Fifty-three patients (50%) died within 30 days of A. baumanniii isolation: 28 (20%) in colonized and 45 (68.2%) in those classified as infection (p<0.001). In multivariate regression analysis, clinical infection and patients with hematologic neoplasm, predicted 30-day mortality. The molecular epidemiology of this outbreak showed the threat posed by the introduction of MDR strains from other institutions in a hospital of immunosuppressed patients and

show abstract

“…From a public health perspective, however, Central America is strategically important within the Region of the Americas because it connects and divides North America and South America ( 9 ). In Honduras, recent studies ( 10 , 11 ) reveal that A. baumannii has novel molecular characteristics of antimicrobial resistance mechanisms, results that raise public health concerns regarding implications for the clinical setting. Furthermore, a newer study in Nicaragua ( 12 ) describes the presence of A. Baumannii carbapenem-resistant strains using molecular tools, underscoring the need to examine the clinical and epidemiological implications of its findings for Central America.…”

mentioning

confidence: 99%

Antimicrobial profile of Acinetobacter baumannii at a tertiary hospital in Honduras: a cross-sectional analysis

Zuniga-Moya

Caballero

Loucel-Linares

et al. 2020

Revista Panamericana De Salud Pública

View full text Add to dashboard Cite

Acinetobacter baumannii is considered to be a worldwide threat to public health due to its high antimicrobial resistance rates and the severe infections it can cause. Little is known about this pathogen’s resistance in Central America. This report aims to describe the antimicrobial resistance profile of A. baumannii at a tertiary hospital in Honduras. The cross-sectional analysis was conducted at the tertiary care laboratory hospital in San Pedro Sula in 2015 – 2017. A total of 113 consecutive microbiological reports were analyzed, comprising 100 individuals from whom A. baumannii was isolated. Epidemiological and microbiological data, including the isolation setting and patient information, were recorded. Prevalence of multi-drug and extensive-drug resistance was assessed according to international standards. The median age of individuals was 22 years (2 – 35 years); female was the predominant gender (53%). The hospital’s pediatric wards had the highest number of isolates (n = 48). The most frequent specimen from which A. baumannii was isolated was skin and soft tissue (n = 39). Resistance to carbapenems was reported to be 40.7% among the isolates (n = 46); multi-drug resistant, 35.4% (n = 40); and extensively-drug resistant, 7.1% (n = 8). This report reveals the threat of this pathogen to public health in Honduras and appeals for antibiotic stewardship programs throughout Central America.

show abstract

Unexplored Genetic Diversity of Multidrug- and Extremely Drug-Resistant Acinetobacter baumannii Isolates from Tertiary Hospitals in Honduras

Cited by 16 publications

References 20 publications

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

Origin of OXA-23 Variant OXA-239 from a Recently Emerged Lineage of Acinetobacter baumannii International Clone V

High mortality in an outbreak of multidrug resistant Acinetobacter baumannii infection introduced to an oncological hospital by a patient transferred from a general hospital

Antimicrobial profile of Acinetobacter baumannii at a tertiary hospital in Honduras: a cross-sectional analysis

Contact Info

Product

Resources

About