Unexpected features of exponentially growing Tobacco Bright Yellow-2 cell suspension culture in relation to excreted extracellular polysaccharides and cell wall composition

Issawi, Mohammad; Muhieddine, Mohammad; Faugeron-Girard, Céline; Sol, Vincent; Riou, Catherine

doi:10.1007/s10719-017-9782-7

Cited by 9 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considering these findings and the previously description of Araucaria PEMs transition (Steiner et al 2015) ours results indicate that pectic substances keep ECs and SCs attached to each other allowing small aggregates become large clusters of proembryogenic masses. Apart from to play a role on maintenance of cells aggregates, polysaccharides and proteins are cell-excreted to the culture medium and modify the pH and conductivity value in tobacco cell suspension (Issawi et al 2017). A similar case can be observed in our work (Fig.…”

Section: Cell Suspension Growth Dynamics: Cells Enter Stress After 15 Days Of Cultivationsupporting

confidence: 88%

Integrated proteomics and histochemical analysis of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) in embryogenic suspension culture

Dias

Steiner

Cangahaula-Inocente

et al. 2020

AFR

View full text Add to dashboard Cite

t Cell suspension culture is a useful in vitro model-system for both scaling up and conserving the Brazilian conifer Araucaria angustifolia. In the present work, cell suspension of Araucaria was subjected to proteomics, biochemical and histochemical analyses. The results revealed new insights underlying the molecular mechanism of proembryogenic masses transition in cell suspension. Embryogenic cell cultures were cultivated in a basal liquid medium modified in a Steward apparatus (orbital agitator). Cell growth dynamics was evaluated using cell volume after sedimentation, fresh weight, mitotic index, conductivity, pH, and the number of proembryogenic masses (PEMs: I, II, III). Histochemical parameters, cell viability, and cell death analyses were performed to pinpoint growth rates. Proteomics analysis was performed using two-dimensional electrophoresis, and protein identification was carried out by MALDI-TOF-TOF tandem mass spectrometry. Cell growth dynamics showed a predominance of PEM III. Maximum slope of the exponential phase growth in fresh weight occurred at exponential phase after 15 days (optimal cultivation time), after which cell viability and pH decreased, thereby allowing the identification of stressrelated proteins. Several metabolism and growth proteins were abundant, such as: cytoskeletal, WOX1, cytokinin-related, and auxin-related proteins acting on cell wall modification, suspensor cell formation, and PEM I to PEM III transition.

show abstract

Section: Cell Suspension Growth Dynamics: Cells Enter Stress After 15 Days Of Cultivationsupporting

confidence: 88%

Integrated proteomics and histochemical analysis of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) in embryogenic suspension culture

Dias

Steiner

Cangahaula-Inocente

et al. 2020

AFR

View full text Add to dashboard Cite

show abstract

“…Nevertheless, the B. cinerea cell wall composition and it's global charge remain controversial. It was hypothesized that this neutrality could allow TPPS to cross the fungal cell wall which is completely different from the tobacco cell wall 30,59,60 . Further analyses, such as the H 2 O 2 content, MDA, MTT, total thiols and microscopy analysis, confirmed fungus stress to the point of dying.…”

Section: Rootsmentioning

confidence: 99%

Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

Ambrosini

Issawi

Sol

et al. 2020

Sci Rep

Self Cite

View full text Add to dashboard Cite

Botrytis cinerea is a necrotic plant fungus that causes gray mold disease in over 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents strong resistance to many fungicides. Thus, new strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination with white light. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea using light. 1.5 µM TPPS completely inhibited mycelial growth. TPPS (12.5 µM) was tested on three grapevine clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds increased thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, 2-month-old grapevine leaves were infected with 4-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the detached leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results suggest a strong potential of photo-treatment against B. cinerea mycelium for future agricultural practices in vineyard or other cultures.

show abstract

“…Nevertheless, the Botrytis cell wall composition and its global charge remain controversial. We hypothesized that this neutrality could allow TPPS to cross the fungal cell wall which is completely different from the tobacco cell wall (Cantu et al 2009;Issawi et al 2017Issawi et al , 2019.…”

Section: Tpps Localization Inside Mycelial Cellsmentioning

confidence: 99%

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Ambrosini¹,

Issawi²,

Sol³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Botrytis cinerea is a necrotic fungal plant pathogen responsible for the gray mold disease on more than 200 crops, including grapevine. Due to its genetic plasticity, this fungus presents a strong resistance to a large spectrum of fungicides. Thus, new fighting strategies against B. cinerea are urgently needed. In this context, antimicrobial photodynamic treatment (APDT) was considered. APDT involves the use of a photosensitizer that generates reactive oxygen species upon illumination. Tetra-4-sulfonatophenyl porphyrin tetra-ammonium (TPPS) was tested on B. cinerea; upon light exposure. 1.5 µM TPPS was shown to completely inhibit mycelial growth. A concentration of 12.5 µM TPPS was tested on three genetic background clones from Chardonnay, Merlot and Sauvignon, grown in vitro for 2 months. Treated root apparatus of the three backgrounds was able to increase thiol production as a molecular protection against photoactivated TPPS, leading to a normal phenotype as compared with control plantlets. Finally, two-month-old grapevine leaves were infected with four-day-old mycelium of B. cinerea pre-incubated or not with TPPS. The pre-treated mycelium was unable to infect the isolated leaves of any of the three grapevine varieties after 72 h growth when subjected to a 16 h photoperiod, contrary to untreated mycelium. These results on fungus, plantlets and Botrytis-grapevine leaves, in contact with a very low concentration of TPPS, suggest a strong potential of photo-treatment against Botrytis mycelium for future agricultural practices in vineyard or other cultures.

show abstract

Unexpected features of exponentially growing Tobacco Bright Yellow-2 cell suspension culture in relation to excreted extracellular polysaccharides and cell wall composition

Cited by 9 publications

References 31 publications

Integrated proteomics and histochemical analysis of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) in embryogenic suspension culture

Integrated proteomics and histochemical analysis of Araucaria angustifolia (Bertol.) Kuntze (Araucariaceae) in embryogenic suspension culture

Photodynamic inactivation of Botrytis cinerea by an anionic porphyrin: an alternative pest management of grapevine

Photodynamic inactivation of Botrytis cinerea for a safe pest management of grapevine

Contact Info

Product

Resources

About