Unexpected Effects of Different Potato Resistance Factors to the Colorado Potato Beetle (Coleoptera: Chrysomelidae) on the Potato Aphid (Homoptera: Aphididae)

Ashouri, Ahmad; Michaud, Dominique; Cloutier, Conrad

doi:10.1603/0046-225x-30.3.524

Cited by 46 publications

(25 citation statements)

References 41 publications

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“…inflorescence stems and flowers) and thus on tissues with high growth or development rates. Preferred infestation by aphids, including M. persicae, of stems, flower buds, and flowers of certain host species has been reported previously (Guldemond et al, 1998;Ashouri et al, 2001), and is possibly triggered by nutrient reallocation to flowers and buds during plant developmental stages. Our finding that VPS52 is highly expressed in these tissues and that VPS52 phloemspecific overexpression negatively impacts aphid virulence suggests that aphid-mediated degradation of VPS52 is important for successful infestation.…”

Section: Discussionmentioning

confidence: 81%

An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence

2017

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Plant-and animal-feeding insects secrete saliva inside their hosts, containing effectors, which may promote nutrient release and suppress immunity. Although for plant pathogenic microbes it is well established that effectors target host proteins to modulate host cell processes and promote disease, the host cell targets of herbivorous insects remain elusive. Here, we show that the existing plant pathogenic microbe effector paradigm can be extended to herbivorous insects in that effector-target interactions inside host cells modify critical host processes to promote plant susceptibility. We showed that the effector Mp1 from Myzus persicae associates with the host Vacuolar Protein Sorting Associated Protein52 (VPS52). Using natural variants, we provide a strong link between effector virulence activity and association with VPS52, and show that the association is highly specific to M. persicae-host interactions. Also, coexpression of Mp1, but not Mp1-like variants, specifically with host VPS52s resulted in effector relocalization to vesicle-like structures that associate with prevacuolar compartments. We show that high VPS52 levels negatively impact virulence, and that aphids are able to reduce VPS52 levels during infestation, indicating that VPS52 is an important virulence target. Our work is an important step forward in understanding, at the molecular level, how a major agricultural pest promotes susceptibility during infestation of crop plants. We give evidence that an herbivorous insect employs effectors that interact with host proteins as part of an effective virulence strategy, and that these effectors likely function in a species-specific manner.

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Section: Discussionmentioning

confidence: 81%

An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence

2017

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“…Their feeding mechanism makes these insects excellent vectors for many plant pathogens, especially viruses, yet less amenable to standard, nonsystemic chemical control by insecticides. Minor effects on the survival and fecundity of aphids reared on Bacillus thuringiensis (Bt) crops have been noted in some studies but not in others (1,3,6). However, the sensitivity of aphids to Bt toxins, or the lack thereof, has not been previously tested through artificial-diet bioassays with exhaustive buffer-based controls.…”

mentioning

confidence: 99%

Effects of Bacillus thuringiensis δ-Endotoxins on the Pea Aphid ( Acyrthosiphon pisum )

Porcar

Grenier

Federici

et al. 2009

Appl Environ Microbiol

107

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“…Thus, the nutritional quality of the insect's diet and associated indirect effects of GM crops may play more significant roles in population development than the presence/absence of BSTI; similar findings have been obtained in Bt-transgenic crops (Ashouri et al, 2001;Clark and Coats, 2006). Food quality has a direct influence on collembolan health and fecundity and Snider (1973) determined that F. candida readily consume or absorb their eggs when they have insufficient food.…”

Section: Discussionmentioning

confidence: 69%

Effects of tobacco genetically modified to express protease inhibitor bovine spleen trypsin inhibitor on non-target soil organisms

O’Callaghan

Brownbridge

Stilwell

et al. 2007

Environ. Biosafety Res.

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Effects of tobacco genetically modified to express the protease inhibitor bovine spleen trypsin inhibitor (BSTI) were examined in laboratory assays against three earthworm and one collembolan species. BSTI is a serine protease inhibitor that can bind to the digestive trypsins of insects feeding on modified plants, resulting in reduced growth and survival. Protease inhibitors are active against a broad range of insects, so may have a large impact on non-target organisms. Survival and fecundity of the collembolan Folsomia candida were unaffected by consumption of artificial diet containing BSTI-expressing tobacco leaf or powdered freeze-dried BSTI-expressing tobacco leaf that was added to soil. Similarly, mortality and growth of earthworms Aporrectodea caliginosa and Lumbricus rubellus did not differ significantly between soil augmented with BSTI-expressing tobacco leaves or unmodified control leaves. The redworm Eisenia fetida gained less weight when provided with BSTI-expressing leaves in one assay, but when the experiment was repeated, there was no significant difference between treatments. BSTI-expressing tobacco and unmodified control leaves decomposed at the same rate, indicating that the inhibitor had no effect on the overall function of the decomposer community of micro-flora and fauna in soil.

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Unexpected Effects of Different Potato Resistance Factors to the Colorado Potato Beetle (Coleoptera: Chrysomelidae) on the Potato Aphid (Homoptera: Aphididae)

Cited by 46 publications

References 41 publications

An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence

An Aphid Effector Targets Trafficking Protein VPS52 in a Host-Specific Manner to Promote Virulence

Effects of Bacillus thuringiensis δ-Endotoxins on the Pea Aphid ( Acyrthosiphon pisum )

Effects of tobacco genetically modified to express protease inhibitor bovine spleen trypsin inhibitor on non-target soil organisms

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