Effects of
            <i>Bacillus thuringiensis</i>
            δ-Endotoxins on the Pea Aphid (
            <i>Acyrthosiphon pisum</i>
            )

Porcar, Manuel; Grenier, Anne-Marie; Federici, Brian A.; Rahbé, Yvan

doi:10.1128/aem.00686-09

Cited by 94 publications

(117 citation statements)

References 11 publications

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“…Insect resistant transgenic crops that express insect-specific toxins derived from the bacterium Bacillus thuringiensis (Bt), have provided effective suppression of lepidopteran (moth) and coleopteran (beetle) pests and have been widely adopted in the United States and elsewhere (2,3). However, the sap-sucking hemipteran pests are not particularly susceptible to the Bt-derived toxins (4)(5)(6). Indeed, in some cases, chemical insecticides have to be applied to curtail damage caused by hemipteran pests on Bt transgenic crops, such that the benefit of using the insect resistant transgenic varieties is lost (7)(8)(9).…”

mentioning

confidence: 99%

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Chougule

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Although transgenic crops expressing Bacillus thuringiensis (Bt) toxins have been used successfully for management of lepidopteran and coleopteran pest species, the sap-sucking insects (Hemiptera) are not particularly susceptible to Bt toxins. To overcome this limitation, we demonstrate that addition of a short peptide sequence selected for binding to the gut of the targeted pest species serves to increase toxicity against said pest. Insertion of a 12-aa pea aphid gut-binding peptide by adding to or replacing amino acids in one of three loops of the Bt cytolytic toxin, Cyt2Aa, resulted in enhanced binding and toxicity against both the pea aphid, Acyrthosiphon pisum, and the green peach aphid, Myzus persicae. This strategy may allow for transgenic plant-mediated suppression of other hemipteran pests, which include some of the most important pests of global agriculture.aphid management | biotechnology | insect resistance

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mentioning

confidence: 99%

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Chougule

Liu

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…Since in one case, when Cry toxin was administered via artificial diet, activity against aphids was demonstrated (Porcar et al 2009), it seems reasonable to expect a similar activity, should the same concentration of toxin be presented via the phloem sap.…”

Section: Discussionmentioning

confidence: 99%

“…In this paper, we report the results of laboratory studies on the effects of GM tomato on survivorship, reproduction and development of the aphid Macrosiphum euphorbiae (Thomas) (Hemiptera: Aphididae). It is of interest to examine the impact of such toxins on aphids because of their pest status (Walgenbrach 1997), but also because of recent claims of Bt endotoxins activity against the pea aphid, Acyrthosiphon pisum (Harris) (Hemiptera: Aphididae) (Porcar et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Interactions betweenBt-expressing tomato and non-target insects: the aphidMacrosiphum euphorbiaeand its natural enemies

Digilio

Sasso²,

Leo

et al. 2012

Journal of Plant Interactions

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Bacillus thuringiensis Berliner toxins are highly specific in terms of insect pest targeted. For example, Cry3Bb is active against Coleoptera. As a consequence, in the framework of using genetically modified plants expressing different Bt toxins in sustainable agriculture, there is a growing interest about the impact of these crops on the environment and on non-target organisms. The effects of genetically modified Bt-tomato plants expressing the toxin Cry3Bb on the aphid Macrosiphum euphorbiae (Thomas) and its natural enemies, the generalist predator Macrolophus caliginosus Wagner and the endoparasitoid wasp Aphidius ervi Haliday, were assessed under laboratory conditions. No significant differences between performance of M. euphorbiae were observed on genetically modified tomato plants (line UC82Bt) with respect to their near-isogenic control line (line UC82). Immunoassays did not detect Cry3Bb protein in M. euphorbiae developing on Bt-expressing tomato plants. Similarly, no significant differences were found on the longevity and prey consumption of M. caliginosus when fed aphids reared on UC82Bt or on UC82. Moreover, the genetic modification did not affect the attractiveness of uninfested tomato plants toward A. ervi. It is therefore concluded that Cry3Bb-expressing tomato plants did not show any acute adverse effects on the biological parameters of the non-target herbivore M. euphorbiae or its natural enemies, M. caliginosus and A. ervi, under laboratory conditions.

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“…B. thuringiensis toxins are specific to a limited number of insect species without any toxicity to humans or other organisms (Bravo et al, 2011). Some of them are toxic to a large number of insect species in this order; Lepidoptera, Diptera and Coleoptera in addition to some Homoptera (MacIntosh et al, 1990;Bravo et al, 2007;Porcar et al, 2009;Palma et al, 2014), and Nematodes (Wei et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis

Fane¹,

Kassogue²,

Traoré³

et al. 2017

Afr. J. Biotechnol.

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The objective of this work was to select the most active Bacillus thuringiensis (Bt) isolated from agricultural soils of Mali through the molecular characterization and the determination of insecticidal activities of the protein crystals, produced by these native isolates. Crystal proteins were extracted from B. thuringiensis culture, and characterized using the SDS-PAGE techniques. Their insecticidal activities were tested using third-instar larvae of Helicoverpa armigera in bioassay tests. The results showed that, of 62 B. thuringiensis treated, 52 isolates showed fragments varying between 10 and 140 kDa on 12% polyacrylamide gel. Cry1 and Cry2 protein crystals were recognized to be effective against Lepidoptera's larvae, which were found in 21% of the tested isolates. In addition to these two expected crystal protein weights, other molecular weights were observed at different proportions, suggesting the presence of other cry genes in the local B. thuringiensis isolates. Four native B. thuringiensis isolates were able to kill 95 to 100% of H. armigera 3rd-instar larvae. Only one native of B. thuringiensis isolate was able to kill 100% of the H. armigera larvae. This is the first study for molecular characterization of Malian native B. thuringiensis isolates, showing the efficacy of the native B. thuringiensis against an important agricultural insect pest.

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Effects of Bacillus thuringiensis δ-Endotoxins on the Pea Aphid ( Acyrthosiphon pisum )

Cited by 94 publications

References 11 publications

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Retargeting of the Bacillus thuringiensis toxin Cyt2Aa against hemipteran insect pests

Interactions betweenBt-expressing tomato and non-target insects: the aphidMacrosiphum euphorbiaeand its natural enemies

Molecular characterization and insecticidal activities of Malian native crystalliferous Bacillus thuringiensis

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