Unexpected diversity of the cestode Echinococcus multilocularis in wildlife in Canada

Introduced and Native Haplotypes ofEchinococcus multilocularisin Wildlife in Saskatchewan, Canada

“…Rectangles indicate newly identified haplotypes in Canada. BC1 is a European-type haplotype recently detected in western Canada Gesy et al 2013). between the central (N2) and Arctic (N1) North American strains (Nakao et al 2009;Gesy et al 2014), and the existence of diversity within the central North American population in the current study, suggests that this is a well-established focus of the parasite and not a recent introduction from the Arctic as previously suggested (Wilson et al 1995).…”

“…There is little information about the genetic diversity of E. multilocularis in Canada, which falls between the N1 and N2 parasite populations. Previously, we reported the results of a single locus approach, which provided a limited amount of genetic information (Gesy et al 2014); here we use a multiple locus approach using a previously published haplotyping scheme that increases the ability to detect diversity and describe relationships within a relatively conserved parasite species (Nakao et al 2009). …”

“…1) using previously described methods (Catalano et al 2012;Gesy et al 2014). Segments from three mtDNA genes were amplified using published primers and protocols for DNA extracted from adult cestodes (Nakao et al 2009).…”

“…Sequence alignment, protein translation, and haplotype network creation were performed as per Gesy et al (2014). Haplotype analysis was performed once with truncated sequences of cob, nad2, and cox1 using the tailored primers and once using the longer sequences from published primers (Nakao et al 2009).…”

Global Distribution of Alveolar and Cystic Echinococcosis

“…the parasite has recently been detected in wolves (Canis lupus) in taiga and boreal regions of the southern Northwest Territories and northern British Columbia (Gesy et al, 2014;Schurer et al, 2014aSchurer et al, , 2016. p0600…”