Unequal gene amplification and transcription in the macronucleus of Tetrahymena pyriformis

Iwamura, Yukio; Sakai, Masaharu; Morita, Takashi; Muramatsu, Masami

doi:10.1021/bi00591a004

Cited by 20 publications

(9 citation statements)

References 13 publications

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“…For electron microscopy, the Tris buffer of the mating mix was removed by either filtration or centrifugation of the cells followed by resuspension in a small volume of distilled water. Cells were fixed at 0" C for 30 Electron-microscopic autoradiography. Cells at different stages of conjugation were filtrated and suspended in 2 ml distilled water and incubated with 50 pCi/ml 3H-uridine (30 Ci/mmol; The Radiochemical Centre, Amersham, England) at 30" for 30 min.…”

Section: Preparation Of Cells For Light and Electron Microscopymentioning

confidence: 99%

Differentiation of nuclear structure during the sexual cycle in Tetrahymena thermophila

Weiske-Benner

Eckert

1985

Differentiation

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Section: Preparation Of Cells For Light and Electron Microscopymentioning

confidence: 99%

Differentiation of nuclear structure during the sexual cycle in Tetrahymena thermophila

Weiske-Benner

Eckert

1985

Differentiation

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“…DNA elimination has been shown to be a part of MAC development in Tetrahymena, first by hybridization kinetics [3,13] and more recently by recombinant DNA analysis. Ten to 20 percent of the MIC genome, mostly repetitive sequences, is eliminated or underreplicated in the MAC [3,13]. Eliminated sequences have been associated with fragmentation at the rDNA locus [ 14,151.…”

Section: Introductionmentioning

confidence: 99%

Macronuclear persistence of sequences normally eliminated during development in Tetrahymena thermophila

White

Allen

1985

Dev. Genet.

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During conjugation in the ciliated protozoan, Tetrahymena thermophilu , a somatic MACronucleus develops from the germinal MICronucleus. Ten to 20 percent of the MIC genome is eliminated during this process. Three repetitive families have been identified which have different levels of repetition in the MIC and are eliminated to different degrees in the MAC. Some members of two of these families persist in the MAC. In this study, we have looked at these persistent sequences in the MAC of cell lines from a variety of sources including several inbed strains, two sets of caryonides, caryonidal subclones, and vegetatively aged cell clones. The results suggest that the sequences that remain in the MAC have a genetic predisposition to persist. However, epigenetic variations occur as the MAC develops so that only some of the persistent sequences are actually observed in a particular MAC. Polymorphisms may be generated if alternative processing of a single MIC segment occurs. These polymorphisms can later be resolved by phenotypic assortment during vegetative growth. These facultatively persistent sequences appear to differ from sequences previously described in this organism.

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“…These changes can be broadly classified as follows : (i) rearrangements (1,2) ; for example the immunoglobulin genes [see review of Dunnick (1), for references]. (ii) amplifications (3)(4)(5)(6)(7)(8)(9) ; for example the mouse dihydrofolate reductase gene (3). (iii) transpositions (10)(11)(12)(13)(14) ; for example the genes which confer different mating types on yeast,[see review of Leupold (10) for references] and (iv) inversions (15)(16)(17); at present described only for prokaryotes, for example the flagellin gene of salmonella (15).…”

Section: Introductionmentioning

confidence: 99%

A detailed comparison of the 5′-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA

1980

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We have examined homologous fragments of DNA cloned from two different tissues for changes in the DNA sequence which might be related to tissue specific gene expression. The 5' end of the chicken ovalbumin gene was cloned from oviduct or erythrocyte DNA using cosmids as vectors. We have compared the two clones obtained by restriction enzyme digestions, analysis of heteroduplexes by electron microscopy or S1 nuclease digestion and by DNA sequencing. Our results show that whereas no alteration occured in the region of the gene assumed to be of importance for the control of transcription, a 4 nucleotide deletion/insertion was detected in the first intron of the ovalbumin gene.

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Unequal gene amplification and transcription in the macronucleus of Tetrahymena pyriformis

Cited by 20 publications

References 13 publications

Differentiation of nuclear structure during the sexual cycle in Tetrahymena thermophila

Differentiation of nuclear structure during the sexual cycle in Tetrahymena thermophila

Macronuclear persistence of sequences normally eliminated during development in Tetrahymena thermophila

A detailed comparison of the 5′-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA

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