A detailed comparison of the 5′-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA

Gannon, Frank; Jeltsch, Jean‐Marc; Perrin, F.

doi:10.1093/nar/8.19.4405

Cited by 20 publications

(7 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The HindlII (panels A and B) and the EcoRI (panel C) digests were hybridized with probe e and h, (Figure 4, line A) respectively. Sites HindlII3 and EcoRI6 are 163 bp apart (Gannon et al, 1980). The same results were obtained when erythrocyte DNA was used instead of oviduct DNA.…”

Section: Cl> Jsupporting

confidence: 67%

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Bellard¹,

Dretzen²,

Bellard³

et al. 1982

The EMBO Journal

View full text Add to dashboard Cite

We examined the chromatin organization of -3 kb of DNA in the 5' -end flanking region of the ovalbumin gene in chicken erythrocyte and oviduct cell nuclei. With specific DNA probes and an indirect end-labeling technique, we analysed the pattern of the DNA fragments obtained after micrococcal nuclease digestion and generated comparative maps of the nuclease cuts. This region of the chicken genome displays a "typical" chromatin arrangement in erythrocyte nuclei, with nucleosomes apparently positioned at random. In contrast, in oviduct nuclei, the same region has an "altered" chromatin structure, and lacks a typical nucleosomal array. The existence of specifically positioned proteins and of alterations in the DNA secondary structure in this region of the oviduct chromatin is suggested by comparison of the nuclease cleavage maps which reveals specific changes: disappearance of nuclease cuts present in "naked" and erythrocyte chromatin DNAs, and appearance of new cuts absent from these DNAs.

show abstract

Section: Cl> Jsupporting

confidence: 67%

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Bellard¹,

Dretzen²,

Bellard³

et al. 1982

The EMBO Journal

View full text Add to dashboard Cite

show abstract

“…The filters were then dried and exposed for autoradiography with Royal X Omat film at -700C by using a Dupont cronex hi plus intensifying screen. Cloning procedures DNA extracted from V 11 F 1 cl.1 subclone 7 was cloned in the cosmid vector pHC 79 (13) essentially as described by Gannon et al (14).…”

Section: Materials and Methods Cellsmentioning

confidence: 99%

Characterization of a gene encoding a 115 K super T antigen expressed by a SV40-transformed rat cell line

1981

View full text Add to dashboard Cite

It has been reported that SV40-transformed V 11 F 1 clone 1 subclone 7 rat cells (subclone 7) produce a super T antigen of 115,000 M .This super T antigen is entirely SV40 coded and is synthesized by traRslation of an elongated form of SV40 early mRNA (May, E., Kress, M., DayaGrosjean, L., Monier, R. and May, P. (1981) J. Virol., 37,[24][25][26][27][28][29][30][31][32][33][34][35] Two recombinant cosmids were constructed by insertion of the 5 Kb and 10 Kb fragments, respectively, into cosmid pHC 79. Using restriction map analysis and nucleotide sequencing, we showed that the 5 Kb fragment actually contained the complete sequence of a gene encoding super T antigen.As compared to the normal SV40 early gene, the sequence of super T gene showed the following rearrangements: (i) The segment between nucleotides 4116 -3544 was duplicated in a direct order and (ii) these two copies of 573 nucleotide sequence were separated by a 93 nucleotide tract which was a nearly perfect inverted repeat of the segment located between nucleotides 4868 and 4776 (nucleotide numbering used here = Weissmann number + 17). INTlR)DUCIONMultiple species of T antigens with molecular weight distinct from normal sized large T antigen can be identified in a variety of SV40 transformed rat and mouse cell lines. Species of T antigen with a molecular weight considerably larger than that of normal-sized large T antigen are widespread in SV40-transformed rat or mouse cells and are referred to as super T antigen (1, 2, 3, 4).As already reported (5) SV40 transformed rat cell line V 11 F 1 clone 1 subclone 7 produces a single species of super T antigen of 115,000Mr in the absence of detectable trace of large T antigen (86,000 Mr). We have previously shown that this super T antigen (super T) is an elongated form of large T antigen, which contains a duplication of that part of large ©) IRL Prm Umited, 1 Falconberg Court, London W1V 5FG, U.K.4111

show abstract

“…SI analysis confirms that S3034a contains a single deletion To strengthen the conclusion that the 74-bp deletion leftward of the Mu-1 insertion was the only change in S3034a relative to its progenitor S3034, it was important to check for nucleotide mismatches throughout the structural gene and flanking regions. If heteroduplexes formed between the two mutant alleles are digested at high SI nuclease concentrations, mismatches as small as 3-5 bp can be detected (Gannon et al, 1980). The 13-kbp full-length clone pMS3034a was linearized at the EcoRI site in pBR322 ( Figure 2C) and annealed to a 32P end-labelled BamHII XhoI fragment ( Figure 2C, fragment d) isolated from plasmid pMS3034.…”

Section: Confirmation Of S3034a Propertiesmentioning

confidence: 99%

A deletion adjacent to the maize transposable element Mu-1 accompanies loss of Adh1 expression.

Taylor¹,

Walbot²

1985

The EMBO Journal

View full text Add to dashboard Cite

Insertion of the maize transposable element Mu‐1 into the first intron of the alcohol dehydrogenase locus (Adh1) of maize produced mutant Adh1‐S3034 with 40% of the wild‐type level of protein and mRNA. Continued instability at this locus resulted in secondary mutations with lower levels of protein expression. One of these, Adh1‐S3034a, has no detectable ADH1 expression. This paper describes the precise nature of the changes in the Adh1 gene that gave rise to the S3034a allele. The Mu‐1 element is still present in the mutant, but Adh1 sequences immediately adjacent to the element are deleted. The deletion starts precisely at the Mu‐1 insertion site and extends 74 bp leftward removing part of the first intron, the intron:exon junction and 2 bp of the eleventh amino acid codon in the first exon of the gene. Tests for reversion within the somatic tissue of plants show that mutant S3034a, unlike its progenitor, is stably null for ADH1 activity.

show abstract

A detailed comparison of the 5′-end of the ovalbumin gene cloned from chicken oviduct and erythrocyte DNA

Cited by 20 publications

References 54 publications

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5′ end of the actively transcribed ovalbumin gene.

Characterization of a gene encoding a 115 K super T antigen expressed by a SV40-transformed rat cell line

A deletion adjacent to the maize transposable element Mu-1 accompanies loss of Adh1 expression.

Contact Info

Product

Resources

About