Unequal distribution of adhesins within populations of Candida albicans

McCourtie, J

doi:10.1016/0378-1097(85)90254-x

Cited by 5 publications

(5 citation statements)

References 5 publications

(16 reference statements)

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“…Therefore it is not surprising that pepstatin A treatment and the disruption of individual SAP genes had only a partial effect on yeast cell adherence. Adhesion is also known to vary significantly between different strains and to be dependent on growth state, growth form, and the carbon source for growth [21, 28]. These factors presumably affect the expression of these various surface adhesins [29].…”

Section: Discussionmentioning

confidence: 99%

Altered adherence in strains ofCandida albicansharbouring null mutations in secreted aspartic proteinase genes

Watts

Cheah

Hube

et al. 1998

FEMS Microbiology Letters

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The aspartate proteinase inhibitor pepstatin A has been shown previously to reduce the adherence of Candida albicans yeast cells to human surfaces. This suggests that in addition to their presumed function facilitating tissue penetration, the secreted aspartate proteinases (Saps) of this fungal pathogen may have auxiliary roles as cellular adhesins. We therefore examined the relative adherence of yeast cells of a parental wild-type strain of C. albicans in relation to yeast cells of strains harbouring specific disruptions in various members of the SAP gene family in an otherwise isogenic background. The adhesiveness of delta sap1, delta sap2, delta sap3 null mutants and a triple delta sap 4-6 disruptant was examined on three surfaces--glass coated with poly-L-lysine or a commercial cell-free basement membrane preparation (Matrigel) and on human buccal epithelial cells. Pepstatin A reduced adherence to all surfaces. Adherence of the each of the single SAP null mutants to these three substrates was either reduced or not affected significantly compared to that of the parental strain. The adherence of the delta sap4-6 mutant was reduced on poly-L-lysine and Matrigel, but increased on buccal cells. The results suggest that in addition to a primary enzymatic role, various SAPs may also act singly or synergistically to enhance the adhesiveness to C. albicans cells to certain human tissues.

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Section: Discussionmentioning

confidence: 99%

Altered adherence in strains ofCandida albicansharbouring null mutations in secreted aspartic proteinase genes

Watts

Cheah

Hube

et al. 1998

FEMS Microbiology Letters

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“…Therefore it is not surprising that pepstatin A treatment and the disruption of individual SAP genes had only a partial e¡ect on yeast cell adherence. Adhesion is also known to vary signi¢cantly between di¡erent strains and to be dependent on growth state, growth form, and the carbon source for growth [21,28]. These factors presumably a¡ect the expression of these various surface adhesins [29].…”

Section: Discussionmentioning

confidence: 99%

Altered adherence in strains of Candida albicans harbouring null mutations in secreted aspartic proteinase genes

Watts¹

1998

FEMS Microbiology Letters

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The aspartate proteinase inhibitor pepstatin A has been shown previously to reduce the adherence of Candida albicans yeast cells to human surfaces. This suggests that in addition to their presumed function facilitating tissue penetration, the secreted aspartate proteinases (Saps) of this fungal pathogen may have auxiliary roles as cellular adhesins. We therefore examined the relative adherence of yeast cells of a parental wild-type strain of C. albicans in relation to yeast cells of strains harbouring specific disruptions in various members of the SAP gene family in an otherwise isogenic background. The adhesiveness of vsap1, vsap2 and vsap3 null mutants and a triple vsap 4^6 disruptant was examined on three surfaces^glass coated with poly-L-lysine or a commercial cell-free basement membrane preparation (Matrigel) and on human buccal epithelial cells. Pepstatin A reduced adherence to all surfaces. Adherence of the each of the single SAP null mutants to these three substrates was either reduced or not affected significantly compared to that of the parental strain. The adherence of the vsap4^6 mutant was reduced on poly-L-lysine and Matrigel, but increased on buccal cells. The results suggest that in addition to a primary enzymatic role, various SAPs may also act singly or synergistically to enhance the adhesiveness to C. albicans cells to certain human tissues. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

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“…Attached cells in turn form hyphae which penetrate tissue (3,10,12). Therefore, the adhesive properties of C. albicans play a role in pathogenesis, and many studies have documented adhesive differences between strains (9, 11,16,18,23). In addition, adhesive differences between the bud and hyphal forms of growth have been demonstrated (3, 14), and in at least one strain, WO-1, adhesive properties vary as a result of high-frequency phenotypic switching (13).…”

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confidence: 99%

Differences in adhesion of Candida albicans 3153A cells exhibiting switch phenotypes to buccal epithelium and stratum corneum

Vargas¹,

Wertz²,

Drake³

et al. 1994

Infect Immun

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Cells of the laboratory strain 3153A of Candida albicans can be stimulated to undergo high-frequency phenotypic switching by a low dose of UV. We have compared the adhesive properties of cells exhibiting the basic original smooth (o-smooth) phenotype and three switch phenotypes (star, irregular wrinkle, and revertant smooth) to buccal epithelium and stratum corneum. The generalized hierarchy of adhesion is as follows: o-smooth > irregular wrinkle > revertant smooth > star. This is the inverse of the hierarchy of the proportions of elongate hyphae formed by these phenotypes in culture. These results suggest that the differences in adhesion between o-smooth and the three switch phenotypes of strain 3153A reflect, at least in part, the level of interference due to the formation of elongate hyphae, which tend to cause clumping in suspension. No major differences in the levels of adhesion of cells of the different phenotypes between buccal epithelium and stratum corneum were observed. Results which demonstrate that buccal epithelium induces germination (hypha formation) by conditioning the medium are also presented.

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Unequal distribution of adhesins within populations of Candida albicans

Cited by 5 publications

References 5 publications

Altered adherence in strains ofCandida albicansharbouring null mutations in secreted aspartic proteinase genes

Altered adherence in strains ofCandida albicansharbouring null mutations in secreted aspartic proteinase genes

Altered adherence in strains of Candida albicans harbouring null mutations in secreted aspartic proteinase genes

Differences in adhesion of Candida albicans 3153A cells exhibiting switch phenotypes to buccal epithelium and stratum corneum

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