Undifferentiated Human Adipose Tissue–Derived Stromal Cells Induce Mandibular Bone Healing in Rats

Parrilla, Claudio; Saulnier, N.; Bernardini, Camilla; Patti, R.; Tartaglione, Tommaso; Fetoni, Anna Rita; Pola, Enrico; Paludetti, Gaetano; Michetti, Fabrizio; Lattanzi, Wanda

doi:10.1001/archoto.2011.61

Cited by 28 publications

(22 citation statements)

References 34 publications

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“…Cell-based approaches are mainly based on mesenchymal stem cells (MSCs), that have been widely employed, in conjunction with appropriate osteoinductive scaffolds, and considered the most effective bioactive bone substitutes “in vivo” [172, 176, 177]. …”

Section: Emerging Strategies For Bone Substitutionmentioning

confidence: 99%

“…It is noteworthy that naïve undifferentiated MSCs are prone to environment-induced lineage commitment [186] meaning that they can undergo spontaneous osteogenic differentiation upon “in vivo ” inoculation into a damaged bone [187, 176]. This feature may suggest their safe use as it does not imply any kind of “ex vivo ” osteogenic induction prior to “in vivo ” inoculation.…”

Section: Emerging Strategies For Bone Substitutionmentioning

confidence: 99%

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Bone substitutes in orthopaedic surgery: from basic science to clinical practice

Campana

Milano

Pagano

et al. 2014

J Mater Sci: Mater Med

848

799

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Bone substitutes are being increasingly used in surgery as over two millions bone grafting procedures are performed worldwide per year. Autografts still represent the gold standard for bone substitution, though the morbidity and the inherent limited availability are the main limitations. Allografts, i.e. banked bone, are osteoconductive and weakly osteoinductive, though there are still concerns about the residual infective risks, costs and donor availability issues. As an alternative, xenograft substitutes are cheap, but their use provided contrasting results, so far. Ceramic-based synthetic bone substitutes are alternatively based on hydroxyapatite (HA) and tricalcium phosphates, and are widely used in the clinical practice. Indeed, despite being completely resorbable and weaker than cortical bone, they have exhaustively proved to be effective. Biomimetic HAs are the evolution of traditional HA and contains ions (carbonates, Si, Sr, Fl, Mg) that mimic natural HA (biomimetic HA). Injectable cements represent another evolution, enabling mininvasive techniques. Bone morphogenetic proteins (namely BMP2 and 7) are the only bone inducing growth factors approved for human use in spine surgery and for the treatment of tibial nonunion. Demineralized bone matrix and platelet rich plasma did not prove to be effective and their use as bone substitutes remains controversial. Experimental cell-based approaches are considered the best suitable emerging strategies in several regenerative medicine application, including bone regeneration. In some cases, cells have been used as bioactive vehicles delivering osteoinductive genes locally to achieve bone regeneration. In particular, mesenchymal stem cells have been widely exploited for this purpose, being multipotent cells capable of efficient osteogenic potential. Here we intend to review and update the alternative available techniques used for bone fusion, along with some hints on the advancements achieved through the experimental research in this field.

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Section: Emerging Strategies For Bone Substitutionmentioning

confidence: 99%

Section: Emerging Strategies For Bone Substitutionmentioning

confidence: 99%

Bone substitutes in orthopaedic surgery: from basic science to clinical practice

Campana

Milano

Pagano

et al. 2014

J Mater Sci: Mater Med

848

799

View full text Add to dashboard Cite

show abstract

“…Interestingly, ASCs proved to allow bone regeneration in vivo , without the need for ex vivo engineering and/or induction [12]. Also, it has been clearly demonstrated that allogeneic ASCs displays a nonimmunogenic profile in vitro and does not evoke cell-based immunity when implanted in a rat spinal fusion model [66].…”

Section: Cell-therapies For Spinal Fusionmentioning

confidence: 99%

“…The improved knowledge on adult stem cell biology and of mesenchymal stem cell features allowed their use in regenerative medicine, with particular focus on bone regeneration. In particular, cell-based approaches based on mesenchymal stem cells (MSC) have been widely employed and considered the most effective for bone formation and regeneration in vivo [12, 13]. In addition, the overwhelming amount of studies that have been investigating the molecular scenario orchestrating osteogenesis and bone healing, provided new osteoinductive molecules to be tested as potential drugs in spine surgery.…”

Section: Introductionmentioning

confidence: 99%

Spinal Fusion in the Next Generation: Gene and Cell Therapy Approaches

Barba

Cicione

Bernardini

et al. 2014

The Scientific World Journal

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Bone fusion represents a challenge in the orthopedics practice, being especially indicated for spine disorders. Spinal fusion can be defined as the bony union between two vertebral bodies obtained through the surgical introduction of an osteoconductive, osteoinductive, and osteogenic compound. Autogenous bone graft provides all these three qualities and is considered the gold standard. However, a high morbidity is associated with the harvest procedure. Intensive research efforts have been spent during the last decades to develop new approaches and technologies for successful spine fusion. In recent years, cell and gene therapies have attracted great interest from the scientific community. The improved knowledge of both mesenchymal stem cell biology and osteogenic molecules allowed their use in regenerative medicine, representing attractive approaches to achieve bone regeneration also in spinal surgery applications. In this review we aim to describe the developing gene- and cell-based bone regenerative approaches as promising future trends in spine fusion.

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“…The authors suggest that the ready availability of these cells following orthopaedic injuries (involving traumatised muscle tissue) makes them an attractive possible cell source for regenerative medicine approaches. Adipose tissues also provide a readily available source of stromal cells, and adipose derived stromal cells have been used to successfully regenerate bone (Parrilla et al 2011;Levi et al 2010).…”

Section: Auto-transplantation and Homing Of Endogenous Cellsmentioning

confidence: 99%

Endogenous musculoskeletal tissue engineering - a focused perspective

Adam

2011

Cell Tissue Res

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Two major difficulties facing widespread clinical implementation of existing Tissue Engineering (TE) strategies for the treatment of musculoskeletal disorders are (1) the cost, space and time required for ex vivo culture of a patient's autologous cells prior to re-implantation as part of a TE construct, and (2) the potential risks and availability constraints associated with transplanting exogenous (foreign) cells. These hurdles have led to recent interest in endogenous TE strategies, in which the regenerative potential of a patient's own cells is harnessed to promote tissue regrowth without ex vivo cell culture. This article provides a focused perspective on key issues in the development of endogenous TE strategies, progress to date, and suggested future research directions toward endogenous repair and regeneration of musculoskeletal tissues and organs.

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Undifferentiated Human Adipose Tissue–Derived Stromal Cells Induce Mandibular Bone Healing in Rats

Abstract: Undifferentiated human ATSCs are suitable for cell-based treatment of mandibular defects, even in the absence of previous osteogenic induction in vitro.

Cited by 28 publications

References 34 publications

Bone substitutes in orthopaedic surgery: from basic science to clinical practice

Bone substitutes in orthopaedic surgery: from basic science to clinical practice

Spinal Fusion in the Next Generation: Gene and Cell Therapy Approaches

Endogenous musculoskeletal tissue engineering - a focused perspective

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