Understanding the Antifungal Mechanism of Ag@ZnO Core-shell Nanocomposites against Candida krusei

Das, Bhaskar; Khan, Md. Imran; Jayabalan, R.; Behera, Susanta Kumar; Yun, Soon‐Il; Tripathy, Suraj K.; Mishra, Amrita

doi:10.1038/srep36403

Cited by 76 publications

(43 citation statements)

References 49 publications

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“…Similar morphological changes to C. krusei were observed in a study using silver nanocompounds. These irregularities were attributed to the ability of the compound to cause cell membrane damage [48]. Another study that evaluated the antifungal effect of the tripeptide FAR (Phe-Ala-Arg) against C. krusei showed similar irregularities on the fungal cell surface due to peptide accumulation in the membrane, causing an increase in permeability and loss of barrier function, which led to cell death [49].…”

Section: Discussionmentioning

confidence: 99%

Promising antifungal activity of new oxadiazole against Candida krusei

et al. 2020

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Candida krusei is one of the most common agents of invasive candidiasis and candidemia worldwide, leading to high morbidity and mortality rates. This species has become a problem due to its intrinsic resistance and reduced susceptibility to azoles and polyenes. Moreover, the number of antifungal drugs available for candidiasis treatment is limited, demonstrating the urgent need for the discovery of novel alternative therapies. In this work, the in vivo and in vitro activities of a new oxadiazole (LMM11) were evaluated against C. krusei. The minimum inhibitory concentration ranged from 32 to 64 μg/mL with a significant reduction in the colony forming unit (CFU) count (~3 log 10). LMM11 showed fungicidal effect, similar to amphotericin, reducing the viable cell number (>99.9%) in the time-kill curve. Yeast cells presented morphological alterations and inactive metabolism when treated with LMM11. This compound was also effective in decreasing C. krusei replication inside and outside macrophages. A synergistic effect between fluconazole and LMM11 was observed. In vivo treatment with the new oxadiazole led to a significant reduction in CFU (0.85 log 10). Furthermore, histopathological analysis of the treated group exhibited a reduction in the inflammatory area. Taken together, these results indicate that LMM11 is a promising candidate for the development of a new antifungal agent for the treatment of infections caused by resistant Candida species such as C. krusei.

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Section: Discussionmentioning

confidence: 99%

Promising antifungal activity of new oxadiazole against Candida krusei

et al. 2020

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“…Silver nanoparticles (AgNPs) are one of the most vital nanomaterials among several metallic nanoparticles that are involved in biomedical applications (Wei et al, 2015; Zhang et al, 2016). They also show antimicrobial activity making them applicable to different areas of medicine with the potential to combat the proliferation of microorganisms and yeasts (Dúran et al, 2010; Iravani, 2011; Vasquez-Munoz, Avalos-Borja & Castro-Longoria, 2014; Jacometo et al, 2015; Szweda et al, 2015; Das et al, 2016). However, there is a growing need for developing environmentally friendly processes of synthesis of nanoparticles that do not use toxic chemicals (Song & Kim, 2009).…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of silver nanoparticles using Caesalpinia ferrea (Tul.) Martius extract: physicochemical characterization, antifungal activity and cytotoxicity

Soares

Corrêa

Stroppa

et al. 2018

PeerJ

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BackgroundGreen synthesis is an ecological technique for the production of well characterized metallic nanoparticles using plants. This study investigated the synthesis of silver nanoparticles (AgNPs) using a Caesalpinia ferrea seed extract as a reducing agent.MethodsThe formation of AgNPs was identified by instrumental analysis, including ultraviolet–visible (UV–Vis) spectroscopy, scanning electron microscopy (SEM), X-ray diffraction (XRD) of the AgNPs, and surface-enhanced Raman scattering (SERS) spectra of rhodamine-6G (R6G). We studied the physicochemical characterization of AgNPs, evaluated them as an antifungal agent against Candida albicans, Candida kruzei, Candida glabrata and Candida guilliermondii, and estimated their minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) values. Lastly, this study evaluated the cytotoxicity of the AgNPs in murine L929 fibroblasts cells using an MTT assay.ResultsThe UV–Vis spectroscopy, SERS, SEM and XRD results confirmed the rapid formation of spheroidal 30–50 nm AgNPs. The MIC and MFC values indicated the antifungal potential of AgNPs against most of the fungi studied and high cell viability in murine L929 fibroblasts. In addition, this study demonstrated that C. ferrea seed extracts may be used for the green synthesis of AgNPs at room temperature for the treatment of candidiasis.

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“…Redshift for the wavelength from 294 nm to 302 nm occurs after coating iron oxide NPs by ZnO NPs (Figure 3). There is no distinctive SPR difference between for the SPR due to distinctive SPR differences between both AgNPs and ZnO NPs (Das et al, 2016;Abhilash et al, 2019). The mean average diameter of ZnO-coated iron oxide NPs was 122.4 nm using DLS, but the PDI was 1 which reflects a broad nanoparticle size distribution and instability (Figure 4).…”

Section: Resultsmentioning

confidence: 98%

“…The mixture was shaken for 1 h and allowed to stand at ambient temperature for half an hour (Mahdavi et al, 2013). Iron oxide NPs were designed by adding 0.2 M ferric chloride to Blepharis extract in a 1:1 volume ratio (Das et al, 2016). Iron oxide nanosuspension was characterized by using a UV spectroscope and Zetasizer.…”

Section: Biological Synthesis Of Iron Oxide Npsmentioning

confidence: 99%

Fabrication of Iron Oxide/Zinc Oxide Nanocomposite Using Creeper Blepharis maderaspatensis Extract and Their Antimicrobial Activity

Abbas

Krishnan

Kotakonda

2020

Front. Bioeng. Biotechnol.

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Green nanotechnology has recently had a significant influence on advances in biological applications. The surface manipulation of iron oxide NPs by zinc oxide is increasing attention for biomedical research. Therefore, this work focused on the phytochemicals of creeper Blepharis maderaspantensis (BM) water extract for synthesizing iron oxide NPs and iron oxide/zinc oxide nanocomposite. The UV spectrum analysis showed a wavelength redshift from 294 to 302 nm of iron oxide/ZnO nanocomposite, and the polydispersity index revealed that the perfect preparations of iron oxide NPs were prepared by boiling 0.25 g of the plant in deionized water then the filtrate added to ferric chloride (1:1 v/v). The HRTEM results also illustrated that amorphous iron oxide NPs are spherical and irregular in shape. However, the iron oxide/ZnO nanocomposite showed a rod shape of ZnO with an average length and width of ∼19.25 ± 3.2 × 3.3 ± 0.6 nm surrounding amorphous iron oxide NPs. Furthermore, a high antimicrobial activity with MRSA and E. coli was demonstrated by iron oxide NPs. However, because of instability and negative surface charge of the iron oxide nanocomposite, there was no antimicrobial activity. Future cytotoxic studies of the iron oxide NPs synthesized with polyphenols of BM extract are desirable, and their applications in medical purposes will be recommended.

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Understanding the Antifungal Mechanism of Ag@ZnO Core-shell Nanocomposites against Candida krusei

Cited by 76 publications

References 49 publications

Promising antifungal activity of new oxadiazole against Candida krusei

Promising antifungal activity of new oxadiazole against Candida krusei

Biosynthesis of silver nanoparticles using Caesalpinia ferrea (Tul.) Martius extract: physicochemical characterization, antifungal activity and cytotoxicity

Fabrication of Iron Oxide/Zinc Oxide Nanocomposite Using Creeper Blepharis maderaspatensis Extract and Their Antimicrobial Activity

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