UNDERSTANDING PHYTOCHEMICAL ROLES ON α-GLUCOSIDASE INHIBITORY ACTIVITY BASED ON METABOLOMIC APPROACH OF Premna serratifolia LEAVES FROM WEST BORNEO, INDONESIA

Hadiarti, Dini; Haryadi, Winarto; Matsjeh, Sabirin; Swasono, Respati Tri

doi:10.31788/rjc.2021.1426320

Cited by 2 publications

(6 citation statements)

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“…The extraction and fractionation procedures were carried out as reported by our research group (Hadiarti et al, 2021). The ethyl acetate fraction (16.24 g) is isolated by column chromatography (44 × 3.5 cm) utilizing eluent gradation from hexane, chloroform, and ethanol (100%:0%-0%:100%).…”

Section: Column Chromatography Of Ethyl Acetate Extract Of P Serratif...mentioning

confidence: 99%

“…Based on our previous research (Hadiarti et al, 2021), the ethyl acetate fraction of P. serratifolia is the best α-glucosidase inhibitor (77.63 ± 0.18 mg Acarbose equivalent (ACAE)/100 g extract) From Figure 1, among 24 subfractions of ethyl acetate, F 12 -F 14 are found to be highly potent inhibitors of α-glucosidase (50.915, 61.041, and 61.746 mmol ACAE/ 100 g extract, respectively). Compared to α-glucosidase inhibition activity of control, F 12 -F 14 subfractions of ethyl acetate are 0.53, 0,63, and 0.64 times than acarbose.…”

Section: α-Glucosidase Inhibitor Activitymentioning

confidence: 99%

“…The identification of α-glucosidase inhibitor from a fractioned subfraction of ethyl acetate by UHPLC-Q-Orbitrap HRMS is presented in Table 1 and Figure 2. The interpretation of spectra MS 1 and MS 2 is focused on flavonoids which have the highest correlation with acarbose equivalent (Hadiarti et al, 2021). There are nine flavonoids divided into three groups which are flavone (centaureidin, chrysin, 3,5,4ʹ-trimethoxy-6,7methylenedioxyflavone, syringetin, pectolinaringenin, tricin, and casticin), isoflavone (glycitein), and flavanol (kaempferide).…”

Section: Profile Of Flavonoidsmentioning

confidence: 99%

“…Traditionally, P. serratifolia leaves have been used as a tonic, treating headache, stomachache, diabetes, hypertension, difficulty in breathing, increasing breast milk production, and malaria (Dianita and Jantan, 2017;Hasanah et al, 2015). The scientific report showed that P. serratifolia leaves have been confirmed as hepatoprotection (Vadivu et al, 2009), antifungal (Wahyuni et al, 2014), antilarval (Lestari and Yanti, 2014), antioxidant (Isnindar et al, 2016), and α-glucosidase inhibitor (Hadiarti, 2017;Hadiarti et al, 2021;Timotius et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Based on our previous study (Hadiarti et al, 2021), the phytochemical screening of ethyl acetate fraction of P. serratifolia leaves contained flavonoids, phenolics, and steroids. The metabolomic approach presented flavonoids which are the best contributor in inhibiting α-glucosidase.…”

Section: Introductionmentioning

confidence: 99%

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Profiling of α-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein-ligand interaction with molecular docking

Hadiarti¹,

Haryadi²,

Matsjeh³

et al. 2022

J App Pharm Sc

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The Buas-buas (Premna serratifolia) leaves' extract from maceration, percolation, and decontamination have proven to be α-glucosidase inhibitors. Since the efforts to isolate phytochemical constituents from P. serratifolia leaves as α-glucosidase inhibitors have not been carried out, a study is needed to determine the responsible compound for this activity which has been proven in vitro and in silico. Isolation of active compounds from ethyl acetate fraction has the best inhibition utilizing column chromatography and semipreparative high-performance liquid chromatography (HPLC). Identifying compounds from the isolate was investigated by ultra-high-performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS). Interaction of α-glucosidase and active compounds was studied by molecular docking utilizing N-terminal maltase-glucoamylase [protein data bank (PDB) code: 2QMJ], C-terminal maltase-glucoamylase (PDB code: 3TOP), and isomaltase (PDB code: 3A4A). Analyzed by UHPLC-Q-Orbitrap HRMS, nine flavonoids were detected, which are centaureidin, chrysin, pectolinaringenin, glycitein, kaempferide, syringetin, tricin, casticin, and 3,5,4ʹ-trimethoxy-6,7-methylenedioxyflavone (estimated to be a new compound). Casticin-2QMJ, tricin-3TOP, and centaureidin-3A4A complexes have the lower binding energies of −5.29, −6.77, and −8.02 kcal/mol and inhibitory constant (Ki) of 131.54, 10.89, and 0.34 µM, sequentially.

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Section: Column Chromatography Of Ethyl Acetate Extract Of P Serratif...mentioning

confidence: 99%

Section: α-Glucosidase Inhibitor Activitymentioning

confidence: 99%

Section: Profile Of Flavonoidsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Profiling of α-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein-ligand interaction with molecular docking

Hadiarti¹,

Haryadi²,

Matsjeh³

et al. 2022

J App Pharm Sc

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Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

Khairunnisa

Febriyanti²,

Muhaimin³

2022

ijbp

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Millions of people worldwide have been using herbal medicines for thousands of years, due to their great interest in traditional medicines. One of the plants in Indonesia which has medicinal properties but is not widely known by the public and used is Bebuas plant. Bebuas (Premna serratifolia Linn) has been observed growing in West Kalimantan. The people of West Kalimantan used bebuas leaves to reduce cholesterol levels, the young leaves used as a traditional medicine to expel wind in the body quickly, treat intestinal worms, and increase breast milk for nursing mothers, and the decoction of fresh leaves is used for colds and fever medicine. Several studies report bebuas (Premna serratifolia L.) had many pharmacological activities. Bebuas contains flavonoids, saponins, tannins and triterpenoids/steroids that have a role in pharmacological activity. The present review aims to summarize the current state of scientific evidence on the pharmacological potentials of P. serratifolia. The subject of literature published from 2012 to 2022 were obtained frominternational research databases including PubMed, Google Scholar, EBSCO, SCOPUS, and Cochrane, using the keywords Premna serratifolia L., traditional medicine, medicinal plants, and pharmacology. The pharmacological activities which are discussed further in the review including antioxidant, anti-arthritic, anticholesterol, anti-inflammatory, antimicrobial, antihelmintic, antidiabetic, and anticancer/cytotoxic. The study suggests that Premna serratifolia L. is a potent source for further development of phytomedicine.

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UNDERSTANDING PHYTOCHEMICAL ROLES ON α-GLUCOSIDASE INHIBITORY ACTIVITY BASED ON METABOLOMIC APPROACH OF Premna serratifolia LEAVES FROM WEST BORNEO, INDONESIA

Cited by 2 publications

References 0 publications

Profiling of α-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein-ligand interaction with molecular docking

Profiling of α-glucosidase inhibitors from ethyl acetate fraction of Buas-buas (Premna serratifolia) leaves using UHPLC-Q-Orbitrap HRMS and protein-ligand interaction with molecular docking

Phytochemistry and Pharmacological Potentials of Premna serratifolia L.: Traditional Medicinal Plant Used by Local People in Kalimantan

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