Understanding Nonidealities of the Osmotic Pressure of Concentrated Bovine Serum Albumin

Yousef, Mohammed; Datta, Ravindra; Rodgers, V.G.J.

doi:10.1006/jcis.1998.5789

Cited by 39 publications

(74 citation statements)

References 30 publications

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“…BSA was the chosen osmotic agent because its concentrationdependent osmotic pressure range is well known 17,18 and its concentration-dependent viscosity is manageable. 19 The BSA is also impermeable to the hollow fibers used (regenerated cellulose, 13 kDa molecular weight cutoff [MWCO], 132294; Spectra/Por Ò ; Spectrum Laboratories, Los Angeles, CA).…”

Section: Osmotic Transport Devicementioning

confidence: 99%

Reduction of Cerebral Edema after Traumatic Brain Injury Using an Osmotic Transport Device

McBride

Szu

Hale

et al. 2014

Journal of Neurotrauma

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Traumatic brain injury (TBI) is significant, from a public health standpoint, because it is a major cause of the morbidity and mortality of young people. Cerebral edema after a TBI, if untreated, can lead to devastating damage of the remaining tissue. The current therapies of severe TBI (sTBI), as outlined by the Brain Trauma Foundation, are often ineffective, thus a new method for the treatment of sTBI is necessary. Herein, the reduction of cerebral edema, after TBI, using an osmotic transport device (OTD) was evaluated. Controlled cortical impact (CCI) was performed on adult female CD-1 mice, and cerebral edema was allowed to form for 3 h, followed by 2 h of treatment. The treatment groups were craniectomy only, craniectomy with a hydrogel, OTD without bovine serum albumin (BSA), and OTD. After CCI, brain water content was significantly higher for animals treated with a craniectomy only, craniectomy with a hydrogel, and OTD without BSA, compared to that of control animals. However, when TBI animals were treated with an OTD, brain water content was not significantly higher than that of controls. Further, brain water content of TBI animals treated with an OTD was significantly reduced, compared to that of untreated TBI animals, TBI animals treated with a craniectomy and a hydrogel, and TBI animals treated with an OTD without BSA. Here, we demonstrate the successful reduction of cerebral edema, as determined by brain water content, after TBI using an OTD. These results demonstrate proof of principle for direct water extraction from edematous brain tissue by direct osmotherapy using an OTD.

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Section: Osmotic Transport Devicementioning

confidence: 99%

Reduction of Cerebral Edema after Traumatic Brain Injury Using an Osmotic Transport Device

McBride

Szu

Hale

et al. 2014

Journal of Neurotrauma

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“…Each of these interactions will have unique hydrations, ion binding values, and fractional amounts of protein which need to be considered. Given that the values of hydration and ion binding are available for all proteins in solution, and that the amount of proteins participating in protein-protein interactions is known, the free-solvent model can provide excellent predictions of the crowded protein osmotic pressure [6,7,30].…”

Section: Strong Protein-protein Interactionsmentioning

confidence: 99%

“…The free-solvent model has been described in detail elsewhere for non-interactive protein solutions [6,7]. The following is the development of the generalized free-solvent model which accounts for protein hydration and ion binding as well as protein-protein interactions.…”

Section: Development Of the Free-solvent Model To Include Protein-promentioning

confidence: 99%

“…van Laar [4] proposed that solvent-solute interactions are coupled to the observed non-ideal behavior of the osmotic pressure and he argued that the mole fraction is the appropriate concentration variable to describe osmotic pressure. Recently, Yousef et al [6,7] revised the free-solvent model to include protein-ion binding. Further, the revised free-solvent model [6,7] describes the solute as a hydrated macromolecule, which contains a monolayer of water and bound ions, and, upon correcting the mole fraction for these interactions, provides excellent predictions for the observed osmotic pressure of single and binary non-interacting protein solutions.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Yousef et al [6,7] revised the free-solvent model to include protein-ion binding. Further, the revised free-solvent model [6,7] describes the solute as a hydrated macromolecule, which contains a monolayer of water and bound ions, and, upon correcting the mole fraction for these interactions, provides excellent predictions for the observed osmotic pressure of single and binary non-interacting protein solutions. Once more, the free-solvent model is based on two independently measurable physical parameters, protein hydration and protein-ion binding, [8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A generalized free-solvent model for the osmotic pressure of multi-component solutions containing protein–protein interactions

McBride

Rodgers

2014

Mathematical Biosciences

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a b s t r a c tThe free-solvent model has been shown to have excellent predictability of the osmotic pressure for single and binary non-interactive proteins in aqueous solutions. Here the free-solvent model is extended to be more generalized by including the contributions of intra-and inter-protein interactions to the osmotic pressure of a solution in the form of homo-and hetero-multimers. The solute-solvent interactions are considered to be unique for each homo-and hetero-multimer in solution. The effect of the various generalized free-solvent model parameters on the osmotic pressure are examined for a single protein solution with a homo-dimer, a binary protein solution with no protein-protein interactions, and a binary protein solution with a hetero-dimer. Finally, the limitations associated with the generalized free-solvent model are discussed.

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Determining fouling‐independent component of critical flux in protein ultrafiltration using the free‐solvent‐based (FSB) model

Wang

Rodgers

2010

AIChE Journal

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Understanding Nonidealities of the Osmotic Pressure of Concentrated Bovine Serum Albumin

Cited by 39 publications

References 30 publications

Reduction of Cerebral Edema after Traumatic Brain Injury Using an Osmotic Transport Device

Reduction of Cerebral Edema after Traumatic Brain Injury Using an Osmotic Transport Device

A generalized free-solvent model for the osmotic pressure of multi-component solutions containing protein–protein interactions

Determining fouling‐independent component of critical flux in protein ultrafiltration using the free‐solvent‐based (FSB) model

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