Understanding intricacies of bioinspired organic-inorganic hybrid nanoflowers: A quest to achieve enhanced biomolecules immobilization for biocatalytic, biosensing and bioremediation applications

Dube, Shubhankar; Rawtani, Deepak

doi:10.1016/j.cis.2021.102484

Cited by 21 publications

(11 citation statements)

References 187 publications

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“…20,21 Among various methods, enzyme−inorganic hybrid nanoflowers with a high specific surface area and good biocompatibility have attracted widespread attention. 22 In addition to the advantages of simple preparation and operation, easy separation, and low cost, nanoflowers also support the co-immobilization of multiple enzymes and have a wide range of uses. 23−26 In this study, a co-immobilized PPK@NK hybrid nanoflower reaction system was constructed and applied to convert uridine, cytidine, and inosine to their corresponding nucleotides in one pot.…”

Section: Introductionmentioning

confidence: 99%

“…Despite being more than a century old, enzyme immobilization is still the unfailing way to achieve greater stability and higher productivity. , The immobilized enzyme allows for recycling, thus reducing the cost of the catalyst. Many immobilization techniques have been developed for the production of high-value-added products. , Among various methods, enzyme–inorganic hybrid nanoflowers with a high specific surface area and good biocompatibility have attracted widespread attention . In addition to the advantages of simple preparation and operation, easy separation, and low cost, nanoflowers also support the co-immobilization of multiple enzymes and have a wide range of uses. − …”

Section: Introductionmentioning

confidence: 99%

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Rational Design to Enhance Enzyme Activity for the Establishment of an Enzyme–Inorganic Hybrid Nanoflower Co-Immobilization System for Efficient Nucleotide Production

Lou

2022

J. Agric. Food Chem.

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Increasing yields while reducing costs is one of the ultimate pursuits of industrial production. To achieve this goal in the enzymatic production of multiple nucleotides, in this study, a co-immobilized polyphosphate kinase–nucleoside kinase hybridized nanoflower system (PPK@NK) was constructed. To improve the productivity, the nucleoside kinase (NK) used was rationally designed, and a variant with significantly increased activity compared to the wild type was obtained. The polyphosphate kinase (PPK) and NK could be sequentially adsorbed on the surface of hybrid nanoflowers at room temperature (25 °C) through the interaction of Cu2+ and proteins without any other chemical pretreatment. The optimal preparation conditions and reaction parameters of PPK@NK hybrid nanoflowers were investigated. Under optimal reaction conditions, the newly prepared co-immobilization system could catalyze the conversion of 100 mM uridine, cytidine, and inosine to the corresponding nucleotides completely within 4 h and could be reused at least six times. The storage stability of the co-immobilized system was more than 2-fold higher than that of the free enzyme, and there was no significant difference in thermostability. PPK@NK hybridized nanoflowers have properties such as easy preparation and storage and low cost, indicating their suitability for the efficient production of nucleotides.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Rational Design to Enhance Enzyme Activity for the Establishment of an Enzyme–Inorganic Hybrid Nanoflower Co-Immobilization System for Efficient Nucleotide Production

Lou

2022

J. Agric. Food Chem.

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“…Another issue that may be addressed by the use of matched patient peripheral blood serum is the use of sophisticated media that often contain fetal bovine serum and require the addition of particular chemicals. This can be avoided by using patient-matched peripheral blood serum [155,156]. For researchers, the addition of fetal bovine serum, a challenging medium, adds another hurdle (FBS).…”

Section: Organoid Culturesmentioning

confidence: 99%

Functional biomaterials for biomimetic 3D in vitro tumor microenvironment modeling

Ahmed

2023

In vitro models

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The translational potential of promising anticancer medications and treatments may be enhanced by the creation of 3D in vitro models that can accurately reproduce native tumor microenvironments. Tumor microenvironments for cancer treatment and research can be built in vitro using biomaterials. Three-dimensional in vitro cancer models have provided new insights into the biology of cancer. Cancer researchers are creating artificial three-dimensional tumor models based on functional biomaterials that mimic the microenvironment of the real tumor. Our understanding of tumor stroma activity over the course of cancer has improved because of the use of scaffold and matrix-based three-dimensional systems intended for regenerative medicine. Scientists have created synthetic tumor models thanks to recent developments in materials engineering. These models enable researchers to investigate the biology of cancer and assess the therapeutic effectiveness of available medications. The emergence of biomaterial engineering technologies with the potential to hasten treatment outcomes is highlighted in this review, which also discusses the influence of creating in vitro biomimetic 3D tumor microenvironments utilizing functional biomaterials. Future cancer treatments will rely much more heavily on biomaterials engineering.

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“…It was reported that the coordination between nitrogen atom of the organic component and the metal ion of the inorganic part is responsible for the formation of HNFs 21 . Due to their interesting properties, HNFs have been widely utilized in biocatalysis, biosensing, and biomedicine 23 . Biosensors based on HNFs provide a lower limit of detection (LOD) and faster response times due to adsorbing and concentrating contaminants surrounding enzymes 24 .…”

Section: Introductionmentioning

confidence: 99%

“…Biosensors based on HNFs provide a lower limit of detection (LOD) and faster response times due to adsorbing and concentrating contaminants surrounding enzymes 24 . HNFs-based biocatalysts have been incorporated for the removal of organic contaminations such as phenolic compounds and industrial dyes 23 . Also, due to the pH-responsive release of the immobilized biomolecule, HNFs have been incorporated as a drug delivery platform 25 .…”

Section: Introductionmentioning

confidence: 99%

Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers

Vojdanitalab

Jafari-Nodoushan

Mojtabavi

et al. 2022

Sci Rep

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A novel approach termed the "concentrated method" was developed for the instant fabrication of laccase@Co3(PO4)2•hybrid nanoflowers (HNFs). The constructed HNFs were obtained by optimizing the concentration of cobalt chloride and phosphate buffer to reach the highest activity recovery. The incorporation of 30 mM CoCl2 and 160 mM phosphate buffer (pH 7.4) resulted in a fast anisotropic growth of the nanomaterials. The purposed method did not involve harsh conditions and prolonged incubation of precursors, as the most reported approaches for the synthesis of HNFs. The catalytic efficiency of the immobilized and free laccase was 460 and 400 M−1S−1, respectively. Also, the enzymatic activity of the prepared biocatalyst was 113% of the free enzyme (0.5 U mL−1). The stability of the synthesized HNFs was enhanced by 400% at pH 6.5–9.5 and the elevated temperatures. The activity of laccase@Co3(PO4)2•HNFs declined to 50% of the initial value after 10 reusability cycles, indicating successful immobilization of the enzyme. Structural studies revealed a 32% increase in the α-helix content after hybridization with cobalt phosphate, which improved the activity and stability of the immobilized laccase. Furthermore, the fabricated HNFs exhibited a considerable ability to remove moxifloxacin as an emerging pollutant. The antibiotic (10 mg L−1) was removed by 24% and 75% after 24 h through adsorption and biodegradation, respectively. This study introduces a new method for synthesizing HNFs, which could be used for the fabrication of efficient biocatalysts, biosensors, and adsorbents for industrial, biomedical, and environmental applications.

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Understanding intricacies of bioinspired organic-inorganic hybrid nanoflowers: A quest to achieve enhanced biomolecules immobilization for biocatalytic, biosensing and bioremediation applications

Cited by 21 publications

References 187 publications

Rational Design to Enhance Enzyme Activity for the Establishment of an Enzyme–Inorganic Hybrid Nanoflower Co-Immobilization System for Efficient Nucleotide Production

Rational Design to Enhance Enzyme Activity for the Establishment of an Enzyme–Inorganic Hybrid Nanoflower Co-Immobilization System for Efficient Nucleotide Production

Functional biomaterials for biomimetic 3D in vitro tumor microenvironment modeling

Instantaneous synthesis and full characterization of organic–inorganic laccase-cobalt phosphate hybrid nanoflowers

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