Understanding angiogenesis and the role of angiogenic growth factors in the vascularisation of engineered tissues

Omorphos, Nicolas Pavlos; Gao, Chuanyu; Tan, Sian See; Sangha, Miljyot Singh

doi:10.1007/s11033-020-06108-9

Cited by 41 publications

(28 citation statements)

References 83 publications

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“…Other important pro-angiogenic factors produced by placenta are the PlGF, which can also activate VEGFR1, neuropilin-1 (NRP-1), angiopoietins, fibroblast growth factor 2, platelet derived growth factor, and the insulin/IGF axis [ 225 , 226 , 227 , 228 ]. As mentioned before, HIF-1α is a major driver of VEGF transcription, however in vitro studies demonstrated PlGF is not regulated by this factor in the human placenta [ 229 ].…”

Section: Endovascular Changes In Gdm: Endothelial Dysfunction and Overstimulation Of Placental Angiogenesismentioning

confidence: 99%

Immunoendocrine Dysregulation during Gestational Diabetes Mellitus: The Central Role of the Placenta

Olmos-Ortíz

Flores-Espinosa

Díaz

et al. 2021

IJMS

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Gestational Diabetes Mellitus (GDM) is a transitory metabolic condition caused by dysregulation triggered by intolerance to carbohydrates, dysfunction of beta-pancreatic and endothelial cells, and insulin resistance during pregnancy. However, this disease includes not only changes related to metabolic distress but also placental immunoendocrine adaptations, resulting in harmful effects to the mother and fetus. In this review, we focus on the placenta as an immuno-endocrine organ that can recognize and respond to the hyperglycemic environment. It synthesizes diverse chemicals that play a role in inflammation, innate defense, endocrine response, oxidative stress, and angiogenesis, all associated with different perinatal outcomes.

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Section: Endovascular Changes In Gdm: Endothelial Dysfunction and Overstimulation Of Placental Angiogenesismentioning

confidence: 99%

Immunoendocrine Dysregulation during Gestational Diabetes Mellitus: The Central Role of the Placenta

Olmos-Ortíz

Flores-Espinosa

Díaz

et al. 2021

IJMS

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“…Interestingly, in breast cancer cells, LHX2 directly controls the expression of Pdgfb by binding to its promoter and controlling endothelial cell migration (Kuzmanov et al, 2014). Beyond VEGF and PDGF, the members of EGF, FGF ( Fgf1 and Fgf2 ), Inhibin and angiopoietin, and the ephrin family are also known to activate angiogenesis in multiple tissues (Gowdak and Krieger, 2018; Gu et al, 2021; Omorphos et al, 2021; Yao et al, 2006). To determine if the migration of endothelial cells in the ovaries in absence of Lhx2 is due to altered expression of these pro-angiogenic factors, we mined the RNAseq data and observed that there is a significant increase in expression of Vegfa , inhibins, Fgf1 , and Fgf2 in the Lhx2 knockout ovaries and their levels are similar or even surpassing to those of the WT testis.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lhx2 in germ cells suppresses endothelial cell migration in the developing ovary

Singh

Bhide

et al. 2022

Preprint

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LIM-homeobox genes play multiple roles in developmental processes, but their roles in gonad development are not completely understood. Herein, we report that Lhx2, Ils2, Lmx1a, and Lmx1b are expressed in a sexually dimorphic manner in mouse, rat, and human gonads during sex determination. Amongst these, Lhx2 has female biased expression in the developing gonads of species with environmental and genetic modes of sex determination. Single-cell RNAseq analysis revealed that Lhx2 is exclusively expressed in the germ cells of the developing mouse ovaries. To elucidate the roles of Lhx2 in the germ cells, we analyzed the phenotypes of Lhx2 knockout XX gonads. While the gonads developed appropriately in Lhx2 knockout mice and the somatic cells were correctly specified in the developing ovaries, transcriptome analysis revealed enrichment of genes in the angiogenesis pathway. There was an elevated expression of several pro-angiogenic factors in the Lhx2 knockout ovaries. The elevated expression of pro-angiogenic factors was associated with an increase in numbers of endothelial cells in the Lhx2-/- ovaries at E13.5. Gonad recombination assays revealed that the increased numbers of endothelial cells in the XX gonads in absence of Lhx2 was due to ectopic migration of endothelial cells in a cell non-autonomous manner. We also found that, there was increased expression of several endothelial cell-enriched male-biased genes in Lhx2 knockout ovaries. Also, in absence of Lhx2, the migrated endothelial cells formed an angiogenic network similar to that of the wild type testis, although the coelomic blood vessel did not form. Together, our results suggest that Lhx2 in the germ cells is required to suppress vascularization in the developing ovary. These results suggest a need to explore the roles of germ cells in the control of vascularization in developing gonads.

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“…From an angiogenesis perspective, growth factors play critical roles in directing cells to initiate and maintain angiogenesis, since they are responsible for transmitting signals between cells and between cells and their microenvironment (Elaimy and Mercurio, 2018;Omorphos et al, 2021). Several growth factors have been widely studied and proven to be effective in promoting vascular regeneration, including VEGF, basic fibroblast growth factor (bFGF), placental growth factor (PLGF) and platelet-derived growth factor-BB (PDGF-BB) (Elaimy and Mercurio, 2018;Hao et al, 2018;Kant and Coulombe, 2018).…”

Section: Growth Factors Stimulating Angiogenesismentioning

confidence: 99%

Rebuilding the Vascular Network: In vivo and in vitro Approaches

Meng

Xing

et al. 2021

Front. Cell Dev. Biol.

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As the material transportation system of the human body, the vascular network carries the transportation of materials and nutrients. Currently, the construction of functional microvascular networks is an urgent requirement for the development of regenerative medicine and in vitro drug screening systems. How to construct organs with functional blood vessels is the focus and challenge of tissue engineering research. Here in this review article, we first introduced the basic characteristics of blood vessels in the body and the mechanism of angiogenesis in vivo, summarized the current methods of constructing tissue blood vessels in vitro and in vivo, and focused on comparing the functions, applications and advantages of constructing different types of vascular chips to generate blood vessels. Finally, the challenges and opportunities faced by the development of this field were discussed.

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Understanding angiogenesis and the role of angiogenic growth factors in the vascularisation of engineered tissues

Cited by 41 publications

References 83 publications

Immunoendocrine Dysregulation during Gestational Diabetes Mellitus: The Central Role of the Placenta

Immunoendocrine Dysregulation during Gestational Diabetes Mellitus: The Central Role of the Placenta

Lhx2 in germ cells suppresses endothelial cell migration in the developing ovary

Rebuilding the Vascular Network: In vivo and in vitro Approaches

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