Rebuilding the Vascular Network: In vivo and in vitro Approaches

Meng, Xiangfu; Xing, Yunhui; Li, Jiawen; Deng, Cechuan; Li, Yifei; Ren, Xi; Zhang, Donghui

doi:10.3389/fcell.2021.639299

Cited by 25 publications

(22 citation statements)

References 128 publications

(139 reference statements)

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“…The vascular network densely permeates organs to provide efficient mass transfer in body[ 1 ]. These branched vessels transfer nutrients and oxygen and withdraw metabolic waste from organs[ 2 ]. The functioning vascular network is essential as well when creating engineered thick tissue.…”

Section: Introductionmentioning

confidence: 99%

Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Nguyen¹,

Duong²,

Hwang³

et al. 2022

ijb

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Rapid construction of pre-vascular structure is highly desired for engineered thick tissue. However, angiogenesis in free-standing scaffold has been rarely reported because of limitation in growth factor (GF) supply into the scaffold. This study, for the 1st time, investigated angiogenic sprouting in free-standing two-vasculature-embedded scaffold with three different culture conditions and additional GFs. A two-core laminar flow device continuously extruded one vascular channel with human umbilical vein endothelial cells (HUVECs) and a 3 mg/ml type-1 collagen, one hollow channel, and a shell layer with 2% w/v gelatin-alginate (70:30) composite. Under the GF flowing condition, angiogenic sprouting from the HUVEC vessel had started since day 1 and gradually grew toward the hollow channel on day 10. Due to the medium flowing, the HUVECs showed elongated spindle-like morphology homogeneously. Their viability has been over 80% up to day 10. This approach could apply to vascular investigation, and drug discovery further, not only to the engineered thick tissue.

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Section: Introductionmentioning

confidence: 99%

Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Nguyen¹,

Duong²,

Hwang³

et al. 2022

ijb

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show abstract

“…In addition, one limitation of the presented study is the lack of quality assessment of the vascular network [51]. Therefore, future experiments might further investigate functional properties like network connectivity and whether the vascular-like structures are indeed hollow and maybe even perfusable.…”

Section: Discussionmentioning

confidence: 98%

Evaluating material-driven regeneration in a tissue engineered human in vitro bone defect model

Wildt

Cramer

Silva

et al. 2023

Bone

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“…Although the main focus was on the defect during the regeneration phase (i.e., from day 28 to day 42), some HUVEC-hBMSC networks had regressed at this stage, based on the fragmented α-smooth muscle actin staining as a supporting cell marker. In addition, one limitation of the presented study is the lack of quality assessment of the vascular network [47]. Therefore, future experiments might further investigate functional properties like network connectivity and whether the vascular-like structures are indeed hollow and maybe even perfusable.…”

Section: Discussionmentioning

confidence: 99%

Evaluating material-driven regeneration in a tissue engineered human in vitro bone defect model

Wildt

Cramer

Silva

et al. 2022

Preprint

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Advanced in vitro human bone defect models can contribute to the evaluation of materials for in situ bone regeneration, addressing both translational and ethical concerns regarding animal models. In this study, we attempted to develop such a model to study material-driven regeneration, using a tissue engineering approach. By co-culturing human umbilical vein endothelial cells (HUVECs) with human bone marrow-derived mesenchymal stromal cells (hBMSCs) on silk fibroin scaffolds with in vitro critically sized defects, the growth of vascular-like networks and three-dimensional bone-like tissue was facilitated. After a model build-up phase of 28 days, materials were artificially implanted and HUVEC and hBMSC migration, cell-material interactions, and osteoinduction were evaluated 14 days after implantation. The materials physiologically relevant for bone regeneration included a platelet gel as blood clot mimic, cartilage spheres as soft callus mimics, and a fibrin gel as control. Although the in vitro model was limited in the evaluation of immune responses, hallmarks of physiological bone regeneration were observed in vitro. These included the endothelial cell chemotaxis induced by the blood clot mimic and the mineralization of the soft callus mimic. Therefore, the present in vitro model could contribute to an improved pre-clinical evaluation of biomaterials while reducing the need for animal experiments.

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Rebuilding the Vascular Network: In vivo and in vitro Approaches

Cited by 25 publications

References 128 publications

Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Angiogenesis in Free-Standing Two-Vasculature-Embedded Scaffold Extruded by Two-Core Laminar Flow Device

Evaluating material-driven regeneration in a tissue engineered human in vitro bone defect model

Evaluating material-driven regeneration in a tissue engineered human in vitro bone defect model

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