Understanding and improving FDM 3D printing to fabricate high-resolution and optically transparent microfluidic devices

Quero, Reverson Fernandes; Silveira, Géssica Domingos da; Silva, José Alberto Fracassi da; Jesus, Dosil Pereira de

doi:10.1039/d1lc00518a

Cited by 60 publications

(45 citation statements)

References 46 publications

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“…As already mentioned, the minimum print resolution depends on many different parameters (design, capabilities of the printer, print material). For example, the structure size is <0.1 mm and the layer height of <0.1 mm and wall thicknesses of <0.1 mm [ 90 ].…”

Section: Methods For Manufacturing Microfluidic Structures For Biosen...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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Proteins in biological fluids (blood, urine, cerebrospinal fluid) are important biomarkers of various pathological conditions. Protein biomarkers detection and quantification have been proven to be an indispensable diagnostic tool in clinical practice. There is a growing tendency towards using portable diagnostic biosensor devices for point-of-care (POC) analysis based on microfluidic technology as an alternative to conventional laboratory protein assays. In contrast to universally accepted analytical methods involving protein labeling, label-free approaches often allow the development of biosensors with minimal requirements for sample preparation by omitting expensive labelling reagents. The aim of the present work is to review the variety of physical label-free techniques of protein detection and characterization which are suitable for application in micro-fluidic structures and analyze the technological and material aspects of label-free biosensors that implement these methods. The most widely used optical and impedance spectroscopy techniques: absorption, fluorescence, surface plasmon resonance, Raman scattering, and interferometry, as well as new trends in photonics are reviewed. The challenges of materials selection, surfaces tailoring in microfluidic structures, and enhancement of the sensitivity and miniaturization of biosensor systems are discussed. The review provides an overview for current advances and future trends in microfluidics integrated technologies for label-free protein biomarkers detection and discusses existing challenges and a way towards novel solutions.

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Section: Methods For Manufacturing Microfluidic Structures For Biosen...mentioning

confidence: 99%

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

et al. 2022

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“…34 The low transparency was likely caused by the high roughness of 3D printed surfaces characterized by large empty spaces between the junctions of the printed layers. 59 Therefore, improving the smoothness of the 3D-printed surfaces might help in enhancing the optical transparency of the devices. Although post-printing methods, such as the treatment of the 3D printed surfaces with organic solvents, have been used to improve the smoothness of the surfaces, this might compromise the resolution if micrometric internal structures are required.…”

Section: Critical Reviewmentioning

confidence: 99%

“…Although post-printing methods, such as the treatment of the 3D printed surfaces with organic solvents, have been used to improve the smoothness of the surfaces, this might compromise the resolution if micrometric internal structures are required. 59 To understand the causes of the low transparency and high roughness in most FDM-based microuidic devices and potentially resolve those issues, Quero et al conducted a theoretical and experimental study. 59 They found that the dimensions of the printer nozzle play a major role in the thermal radiation effects that limit the 3D printing resolution (Fig.…”

Section: Critical Reviewmentioning

confidence: 99%

“…59 To understand the causes of the low transparency and high roughness in most FDM-based microuidic devices and potentially resolve those issues, Quero et al conducted a theoretical and experimental study. 59 They found that the dimensions of the printer nozzle play a major role in the thermal radiation effects that limit the 3D printing resolution (Fig. 5Ai and 5Aii).…”

Section: Critical Reviewmentioning

confidence: 99%

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Emerging 3D printing technologies and methodologies for microfluidic development

2022

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“…For example, one of the main ways to modify FDM printers is through their nozzles because they are the final part in contact with the filament before it is extruded. [ 65 ] Figure 5 A shows a modified FDM printing process to build a colorimetric enzyme‐linked immunosorbent assay (ELISA) to detect malaria. [ 66 ] Different print settings including layer height, extrusion speed as well as extrusion temperature were changed to improve bonding between layers.…”

Section: Manufacturing Principles and Approachesmentioning

confidence: 99%

Micro/Nanofluidic‐Enabled Biomedical Devices: Integration of Structural Design and Manufacturing

Yin

Kim

2021

Advanced NanoBiomed Research

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Micro/nanofluidic devices and systems have attracted ever‐growing attention in healthcare applications over the past decades due to low‐cost yet easy‐customizable functions with the demand of only a small volume of sample fluid. The continuous development, in particular, supported by the emergence of new materials, capable of meeting critical needs in next‐generation, wearable, and multifunctional biomedical devices for at‐home, personalized healthcare monitoring, is challenging the principles and strategies of structural design, manufacturing, and their seamless integration. This review summarizes the progress in micro/nanofluidic‐enabled biomedical devices with a focus on structural design, manufacturing, and applications in healthcare. Structures of fluidic channels and liquid actuation strength are given to elucidate the manipulations and controls of fluid transports that help capture desirable information of interest, including component separation, extraction, measurements, and disease diagnoses. Manufacturing processes of fluidic devices in micro‐ and nanoscales and their basic working principles are also presented, ranging from lithography in traditional hard materials to 3D printing in emerging soft materials. The selected examples and demonstrations are illustrated to highlight applications of biomedical fluidic devices in a broad variety of disease detection and diagnosis. The associated challenges and future opportunities are discussed.

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Understanding and improving FDM 3D printing to fabricate high-resolution and optically transparent microfluidic devices

Abstract: The fabrication of microfluidic devices through Fused Deposition Modeling (FDM) 3D printing has faced several challenges, mainly regarding obtaining microchannels with suitable transparency and sizes. Thus, the use of this...

Cited by 60 publications

References 46 publications

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Label-Free Physical Techniques and Methodologies for Proteins Detection in Microfluidic Biosensor Structures

Emerging 3D printing technologies and methodologies for microfluidic development

Micro/Nanofluidic‐Enabled Biomedical Devices: Integration of Structural Design and Manufacturing

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