Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics

Gac, Séverine Le; Ferraz, Marcia de Almeida Monteiro Melo; Venzac, Bastien; Comizzoli, Pierre

doi:10.1016/j.tibtech.2020.08.012

Cited by 11 publications

(8 citation statements)

References 84 publications

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“…The American mink not only competes for the same ecological niche as the European mink, but it also poses a threat as a vehicle for infectious pathologies [ 22 , 23 , 24 ]. On the other hand, reproductive management and reproductive methodologies in breeding centers have limitations in obtaining sufficient healthy litters for release into natural habitats [ 11 , 25 , 26 ] and in ensuring appropriate genetic diversity.…”

Section: Discussionmentioning

confidence: 99%

“…The value of animal cell biobanks containing germ cells or embryos in assisted reproductive technologies cannot be understated. Germplasm banks contain embryos and gametes that have been preserved to address the lack of contact between animals caused by low densities in natural habitats, long distances between artificial habitats in conservation programs, infertility issues caused by low genetic diversity [ 11 ], or difficulties associated with reproductive management of wild animals, among other issues. Methods for the collection and cryopreservation of sperm, as well as artificial insemination with either fresh or frozen–thawed material for mustelids, have mostly been developed in domestic and black-footed ferrets [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

Calle

Ramírez

2022

IJMS

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The European mink (Mustela lutreola) is one of Europe’s most endangered species, and it is on the brink of extinction in the Iberian Peninsula. The species’ precarious situation requires the application of new ex situ conservation methodologies that complement the existing ex situ and in situ conservation measures. Here, we report for the first time the establishment of a biobank for European mink mesenchymal stem cells (emMSC) and oocytes from specimens found dead in the Iberian Peninsula, either free or in captivity. New emMSC lines were isolated from different tissues: bone marrow (emBM-MSC), oral mucosa (emOM-MSc), dermal skin (emDS-MSC), oviduct (emO-MSc), endometrium (emE-MSC), testicular (emT-MSC), and adipose tissue from two different adipose depots: subcutaneous (emSCA-MSC) and ovarian (emOA-MSC). All eight emMSC lines showed plastic adhesion, a detectable expression of characteristic markers of MSCs, and, when cultured under osteogenic and adipogenic conditions, differentiation capacity to these lineages. Additionally, we were able to keep 227 Cumulus-oocyte complexes (COCs) in the biobank, 97 of which are grade I or II. The European mink MSC and oocyte biobank will allow for the conservation of the species’ genetic variability, the application of assisted reproduction techniques, and the development of in vitro models for studying the molecular mechanisms of infectious diseases that threaten the species’ precarious situation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

Calle

Ramírez

2022

IJMS

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“…On the female side, microfluidic methods offer new capabilities to automate oocyte and embryo analysis, 44–46 with the goal of streamlining the clinical process and reducing associated stresses that impacts the live birth rate. Weng et al 47 developed a microfluidic platform for removing the cumulus–corona cell mass from mouse oocytes – a crucial step to prepare the oocyte for injection prior to ICSI.…”

Section: Microfluidics For Fertilitymentioning

confidence: 99%

Lab on a chip devices for fertility: from proof-of-concept to clinical impact

Nosrati

2022

Lab Chip

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“…Conservation breeding and ARTs are precious assets in the struggle to save endangered wild animal species. In the near future, innovative approaches, such as microfluidic-based in vitro culture or IVF-on-a-chip, may be integrated into conservation programs and crucially contribute to the conservation of endangered species [ 98 ].…”

Section: New Generation Technologies For Sperm Sex-sorting: Microfluidics and Nanotechnologymentioning

confidence: 99%

Game-Changing Approaches in Sperm Sex-Sorting: Microfluidics and Nanotechnology

Neculai-Văleanu

Ariton²

2021

Animals

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The utilization of sex-sorted sperm for artificial insemination and in-vitro fertilization is considered a valuable tool for improving production efficiency and optimizing reproductive management in farm animals, subsequently ensuring sufficient food resource for the growing human population. Despite the fact that sperm sex-sorting is one of the most intense studied technologies and notable progress have been made in the past three decades to optimize it, the conception rates when using sex-sorted semen are still under expectations. Assisted reproduction programs may benefit from the use of emergent nano and microfluidic-based technologies. This article addresses the currently used methods for sperm sex-sorting, as well as the emerging ones, based on nanotechnology and microfluidics emphasizing on their practical and economic applicability.

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Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics

Cited by 11 publications

References 84 publications

Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

Cryobanking European Mink (Mustela lutreola) Mesenchymal Stem Cells and Oocytes

Lab on a chip devices for fertility: from proof-of-concept to clinical impact

Game-Changing Approaches in Sperm Sex-Sorting: Microfluidics and Nanotechnology

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