Abstract:Abstract. The review examines certain aspects of the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH) in living organisms of different taxa, from bacteria to humans. Particular emphasis is put to the role of G6PDH in iron homeostasis and lipogenesis. Prooxidant and antioxidant roles of G6PDH are also highlighted along with several currently known mechanisms of G6PDH regulation.
“…Along with IDH and G6PDH, it can also be oxidatively modified by ROS or protected by the first-line antioxidant enzymes, SOD and catalase ( Figure 8 ). On the other hand, operation of G6PDH and IDH maintains NADPH pools and, in turn, NADPH is required for the assembly of iron-sulfur clusters [ 50 , 51 ]. Figure 8.…”
Objectives
Many plant-derived anti-aging preparations influence antioxidant defense system. Consumption of food supplemented with chili pepper powder was found to extend lifespan in the fruit fly,
Drosophila melanogaster
. The present study aimed to test a connection between life-extending effect of chili powder and antioxidant defense system of
D. melanogaster
.
Methods
Flies were reared for 15 days in the mortality cages on food with 0% (control), 0.04%, 0.12%, 0.4%, or 3% chili powder. Antioxidant and related enzymes, as well as oxidative stress indices were measured.
Results
Female flies that consumed chili-supplemented food had a 40–60% lower glutathione-
S
-transferase (GST) activity as compared with the control cohort. Activity of superoxide dismutase (SOD) was about 37% higher in males that consumed food with 3% chili powder in comparison with the control cohort. Many of the parameters studied were sex-dependent.
Conclusions
Consumption of chili-supplemented food extends lifespan in fruit fly cohorts in a concentration- and gender-dependent manner. However, this extension is not mediated by a strengthening of antioxidant defenses. Consumption of chili-supplemented food does not change the specific relationship between antioxidant and related enzymes in
D. melanogaster
, and does not change the linkage of the activities of these enzymes to fly gender.
“…Along with IDH and G6PDH, it can also be oxidatively modified by ROS or protected by the first-line antioxidant enzymes, SOD and catalase ( Figure 8 ). On the other hand, operation of G6PDH and IDH maintains NADPH pools and, in turn, NADPH is required for the assembly of iron-sulfur clusters [ 50 , 51 ]. Figure 8.…”
Objectives
Many plant-derived anti-aging preparations influence antioxidant defense system. Consumption of food supplemented with chili pepper powder was found to extend lifespan in the fruit fly,
Drosophila melanogaster
. The present study aimed to test a connection between life-extending effect of chili powder and antioxidant defense system of
D. melanogaster
.
Methods
Flies were reared for 15 days in the mortality cages on food with 0% (control), 0.04%, 0.12%, 0.4%, or 3% chili powder. Antioxidant and related enzymes, as well as oxidative stress indices were measured.
Results
Female flies that consumed chili-supplemented food had a 40–60% lower glutathione-
S
-transferase (GST) activity as compared with the control cohort. Activity of superoxide dismutase (SOD) was about 37% higher in males that consumed food with 3% chili powder in comparison with the control cohort. Many of the parameters studied were sex-dependent.
Conclusions
Consumption of chili-supplemented food extends lifespan in fruit fly cohorts in a concentration- and gender-dependent manner. However, this extension is not mediated by a strengthening of antioxidant defenses. Consumption of chili-supplemented food does not change the specific relationship between antioxidant and related enzymes in
D. melanogaster
, and does not change the linkage of the activities of these enzymes to fly gender.
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