Chili-supplemented food decreases glutathione-<i>S</i>-transferase activity in <i>Drosophila melanogaster</i> females without a change in other parameters of antioxidant system

Semaniuk, Uliana; Gospodaryov, Dmytro V.; Strilbytska, Olha; Kucharska, Alicja Z.; Sokół‐Łętowska, Anna; Burdyliuk, Nadia; Storey, Kenneth B.; Bayliak, Maria M.; Lushchak, Oleh

doi:10.1080/13510002.2022.2123884

Cited by 3 publications

(1 citation statement)

References 51 publications

(64 reference statements)

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“…However, the effect may depend on the age of flies, the developmental stage exposed to the treatment, and the strain of the fruit flies (Bayliak et al, 2017). Glutathione‐ S ‐transferase (GST) was shown to be suppressed by preparations of chili pepper that extended lifespan in D. melanogaster (Semaniuk et al, 2022a, 2022b). It was also shown that different isoenzymes of GST can be differentially regulated and affected by circadian rhythms (Wong et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Demianchuk,

Lylyk,

Balatskiy

et al. 2024

Arch Insect Biochem Physiol

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Studies on antiaging remedies in insect models sometimes show discrepancies in results. These discrepancies could be explained by different responses of short‐ and long‐lived strains on the antiaging remedies. The purpose of the study was to test whether life‐prolonging effects of alpha‐ketoglutarate (AKG), observed in nematodes and fruit flies, would be reproduced in long‐lived Drosophila melanogaster flies. Lifespan was assayed in flies kept in demographic cages. Fecundity, proportion of flies capable of negative geotaxis, starvation resistance, time of heat coma onset, levels of triacyglycerols, body glucose, glycogen, activities of glutamate dehydrogenase, catalase, glutathione‐S‐transferase, hexokinase, phosphofructokinase, pyruvate kinase, lactate, and glutamate dehydrogenases were assessed. Dietary AKG did not affect fly lifespan on the diet with 5% yeast and 5% sucrose (5Y:5S) and on the diet with 9% yeast and 1% sucrose (9Y:1S), but increased lifespan on the low‐protein diet (1Y:9S). Twenty‐five‐day‐old female flies fed a 5Y:5S diet with 10 mM AKG for 3 weeks, did not differ from the control group (without AKG) in climbing activity, resistance to heat stress, and starvation. The levels of glucose and glycogen were unaffected but the levels of triacylglycerols were lower in AKG‐fed female flies. No differences in activities of glycolytic enzymes, NADPH‐producing enzymes, glutamate dehydrogenase, oxygen consumption, and levels of oxidative stress markers were observed between the control and AKG‐fed flies. However, AKG‐fed flies had lower activities of catalase and glutathione‐S‐transferase. These results suggest that potential antiaging remedies, such as AKG, may not extend lifespan in long‐living organisms despite influencing several metabolic parameters.

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Section: Discussionmentioning

confidence: 99%

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Demianchuk,

Lylyk,

Balatskiy

et al. 2024

Arch Insect Biochem Physiol

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Supplementation with Thymbra spicata extract ameliorates lifespan, body-weight gain and Paraquat-induced oxidative stress in Drosophila melanogaster: An age- and sex-related study

Diab,

Beghelli,

Nuccitelli

et al. 2024

Journal of Functional Foods

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Proanthocyanidins supplemented diet alter anti-aging-markers and improved lifespan in Drosophila melanogaster model

Jaafaru,

Muhammad,

Mohammed

et al. 2024

Beni-Suef Univ J Basic Appl Sci

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Background It is unequivocally believed that phenolics and flavonoids from fruits and vegetables hold robust prevention potentials against age-related disease development through their abundant hydroxyl groups. This study explored the potential neuromuscular enhancement and anti-aging effects of dietary supplemented proanthocyanidins-rich fraction from Tamarindus indica on Drosophila melanogaster model. One- to three-day-old male and female D. melanogaster were fed with a proanthocyanidins-rich fraction-supplemented diet for 7 days at two different concentrations. Following the effective dose determination, longevity assay (rate of survival), behavioral assay (negative geotaxis and eclosion), and biochemical assays (aging and antioxidant enzymes activities) were conducted to assess the fraction’s longevity, antioxidant, and anti-aging effects on D. melanogaster model. Result The results showed a significant (p < 0.05) improvement in the rate of emergence and lifespan of the flies fed with proanthocyanidins-rich fraction-supplemented diet at both concentrations (1.5 mg/g and 2.5 mg/g) compared to the normal control. A significant decrease in acetylcholinesterase (AChE) activity and the level of caspase-3 and caspase-9 were observed in the D. melanogaster flies fed with the fraction-containing diet when compared with the normal control. The supplemented diet also significantly increases the activity of catalase, superoxide dismutase (SOD), and glutathione-s-transferase (GST) in a concentration-dependent manner but not nicotinamide quinone oxidoreductase one (NQO1) in D. melanogaster upon comparison with the normal control. Conclusion The observable changes in the experiment were attributed to the T. indica-derived proanthocyanidins, flavonoids with robust biological activities. The flavonoid-rich fraction proved its potential by enhancing the antioxidant system in D. melanogaster via the increase in the activities of some of the phase II antioxidant enzymes. The present study provides more insights into the wider perspectives of societies on the use of plant-derived natural compounds as the potential approach toward prevention against aging and age-related morbidities which enhance wellness and the quality of life in humans and animals.

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Chili-supplemented food decreases glutathione-S-transferase activity in Drosophila melanogaster females without a change in other parameters of antioxidant system

Cited by 3 publications

References 51 publications

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Alpha‐ketoglutarate supplementation in long‐lived Drosophila melanogaster: Impact on lifespan and metabolic responses

Supplementation with Thymbra spicata extract ameliorates lifespan, body-weight gain and Paraquat-induced oxidative stress in Drosophila melanogaster: An age- and sex-related study

Proanthocyanidins supplemented diet alter anti-aging-markers and improved lifespan in Drosophila melanogaster model

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