Umbilical cord mesenchymal stem cells combined with secretome for treating congenital pseudarthrosis of the Tibia: a case series

Kurniawan, Aryadi; Ivansyah, Muhammad Deryl; Dilogo, Ismail Hadisoebroto; Hutami, Witantra Dhamar

doi:10.1007/s00590-023-03511-3

Cited by 3 publications

(4 citation statements)

References 22 publications

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“…Similar results have been recently reported by using MSCs from umbilical cord in patients with CPT. 22 Importantly, bone union without refracture was observed in the patient. Likewise, there was not any local or systemic adverse effects after transplantation of the OstO, supporting previous data about the safety of the locally administration of allogeneic MSCs.…”

Section: Discussionmentioning

confidence: 99%

Osteogenic organoid for bone regeneration: Healing of bone defect in congenital pseudoarthrosis of the tibia

Cardier,

Diaz-Solano,

Wittig

et al. 2024

Int J Artif Organs

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Background: Congenital pseudoarthrosis of the tibia (CPT) is an uncommon disease associated with failure to achieve bone union and recurrent fractures. There is evidence showing that CPT is associated with decreased osteogenesis. Based on the capacity of mesenchymal stromal cells (MSCs) to induce osteogenesis, we develop an osteogenic organoid (OstO) constituted by these cells, and other components of the bone niche, for inducing bone formation in a child diagnosed with CPT. Aim: To evaluate the capacity of an OstO to induce bone formation in a patient with CPT. Methods: The OstO was fabricated with allogeneic bone marrow MSCs from a healthy donor, collagen microbeads (CM) and PRP clot. The CM and PRP function as extracellular matrix and scaffolds for MSC. The OstO was placed at the site of non-union. Internal and external fixation was placed in the tibia. Radiological evaluation was performed after MSCs transplantation. Results: After 4 months of MSCs transplantation, radiographic imaging showed evidence of osteogenesis at the site of CPT lesion. The tibia showed bone consolidation and complete healing of the non-union CPT lesion after 6 months. Functional improvement was observed after 1 year of MSC transplantation. Conclusions: The OstO is a bone-like niche which promote osteogenesis in patients with failure in bone formation, such as CPT. To our knowledge, these results provide the first evidence showing CPT healing induced by an OstO constituted by allogeneic MSCs. Future studies incorporating a larger number of patients may confirm these results.

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Section: Discussionmentioning

confidence: 99%

Osteogenic organoid for bone regeneration: Healing of bone defect in congenital pseudoarthrosis of the tibia

Cardier,

Diaz-Solano,

Wittig

et al. 2024

Int J Artif Organs

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“…Mesenchymal stem cell (MSC) transplants in skeletal defects play essential roles of enhancing bone regeneration and vascularization. The transplanted cells can differentiate into functional cells and secrete an extracellular matrix or cytokines and growth factors to stimulate osteogenic and angiogenic cells [1][2][3]. As a result, they promote the tissue regeneration capacity of host bone and bone substitutes [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…A direct role of the transplanted MSCs in bone regeneration is demonstrated by the identification of cells and proteins of a human origin in the matrix of the newly formed bone in animal models [4,8]. Additionally, the importance of the paracrine effects is further highlighted by the biologic effects of the exosomes from MSCs that could promote osteogenic differentiation and bone regeneration, and attenuate the inflammatory response [1][2][3]9]. The role of a stem cell transplant in enhancing the regenerative capacity of host bone become more essential in elderly and medically compromised patients with osteoporosis, large bone defects, and nonunion fractures, etc.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation

Arpornmaeklong,

Boonyuen,

Apinyauppatham

et al. 2024

Bioengineering

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Introduction: To develop a stem cell delivery model and improve the safety of stem cell transplantation for bone regeneration, this study aimed to determine the effects of stem cell sources, serum-free cell culture, and hydrogel cell encapsulation on the growth and osteogenic differentiation of mesenchymal stem cells (MSCs) from the oral cavity. Methods: The study groups were categorized according to stem cell sources into buccal fat pad adipose (hBFP-ADSCs) (Groups 1, 4, and 7), periodontal ligament (hPDLSCs) (Groups 2, 5, and 8), and dental pulp-derived stem cells (hDPSCs) (Groups 3, 6, and 9). MSCs from each source were isolated and expanded in three types of sera: fetal bovine serum (FBS) (Groups 1–3), human serum (HS) (Groups 4–6), and synthetic serum (SS) (StemPro™ MSC SFM) (Groups 7–9) for monolayer (m) and hydrogel cell encapsulation cultures (e). Following this, the morphology, expression of MSC cell surface antigens, growth, and osteogenic differentiation potential of the MSCs, and the expression of adhesion molecules were analyzed and compared. Results: SS decreased variations in the morphology and expression levels of cell surface antigens of MSCs from three cell sources (Groups 7m–9m). The levels of osteoblastic differentiation of the hPDLSCs and hBFP-ADSCs were increased in SS (Groups 8m and 7m) and the cell encapsulation model (Groups 1e, 4e, 7e–9e), but the promoting effects of SS were decreased in a cell encapsulation model (Groups 7e–9e). The expression levels of the alpha v beta 3 (ITG-αVβ3) and beta 1 (ITG-β1) integrins in the encapsulated cells in FBS (Group 1e) were higher than those in the SS (Group 7e). Conclusions: Human PDLSCs and BFP-ADSCs were the optimum stem cell source for stem cell encapsulation by using nanohydroxyapatite–calcium carbonate microcapsule–chitosan/collagen hydrogel in serum-free conditions.

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Application of mesenchymal stem cells derived from the umbilical cord or Wharton’s jelly and their extracellular vesicles in the treatment of various diseases

Patel,

Mohamed,

Rizaev

et al. 2024

Tissue and Cell

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Umbilical cord mesenchymal stem cells combined with secretome for treating congenital pseudarthrosis of the Tibia: a case series

Cited by 3 publications

References 22 publications

Osteogenic organoid for bone regeneration: Healing of bone defect in congenital pseudoarthrosis of the tibia

Osteogenic organoid for bone regeneration: Healing of bone defect in congenital pseudoarthrosis of the tibia

Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation

Application of mesenchymal stem cells derived from the umbilical cord or Wharton’s jelly and their extracellular vesicles in the treatment of various diseases

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