Umbelliprenin-coated Fe3O4 magnetite nanoparticles: Antiproliferation evaluation on human Fibrosarcoma cell line (HT-1080)

Abstract

The potential applications of Fe 3 O 4 magnetite nanoparticles (MNPs) in nanomedicine as drug delivery systems are well known. In this study we prepared umbelliprenin-coated Fe 3 O 4 MNPs and evaluated the antiproliferative effect of combination in vitro. After synthesis of Fe 3 O 4 MNPs, particles were characterized by transmission electron microscopy, energy-dispersive spectroscopy, and X-ray diffraction spectroscopy techniques. The natural candidate compoundumbelliprenin-was isolated and identified and umbelliprenin-coated Fe 3 O 4 MNPs were prepared, using precipitation method. The surface chemistry of umbelliprenin-coated Fe 3 O 4 MNPs as well as their thermal decomposition characteristics was examined using Fourier transform infrared spectroscopy and Thermogravimetric Analyzer equipment, respectively. HT-1080 cells were cultured until the logarithmic phase of growth, and MTT assay was successfully carried out to evaluate the possible cytotoxic effects of umbelliprenin-coated Fe 3 O 4 MNPs in viable cells in vitro. The results demonstrated that umbelliprenin has moderate antiproliferative effects with IC 50 value of 50 µg/mL. However, the combination of umbelliprenin and Fe 3 O 4 MNPs showed the IC 50 value of 9 µg/mL. In other words, cell proliferation decreased to the remarkably-low proportion of 45% after treating cells with umbelliprenin-coated Fe 3 O 4 MNPs. This suggests that with the aid of nanoparticles as carriers, natural products may have even broader range of medical applications in future.

Preparation and evaluation of the effect of Fe3O4@piroctone olamine magnetic nanoparticles on matrix metalloproteinase‐2: A preliminary in vitro study

“…The obtained results showed that the addition of PO on the surface of the magnetic NPs increased the size of related nanoparticles. Such effect was previously reported for the coating of the magnetic NPs with other organic compound such as umbelliprenin .…”

“…Fe 3 O 4 NPs were synthesized using a modified reported method consisting of the alkalization of aqueous solution containing Fe 2+ and Fe 3+ ions in a deoxygenated condition . Briefly, deionized water was boiled for 15 Min and then cooled to room temperature.…”

Fabrication of docetaxel surfaced Fe3O4 magnetite nanoparticles and their cytotoxicity on 4 T1 breast cancer cells

“…Then, 15 μl MTT (0.5 mg/ml) was added to each well, and the plates were incubated for 2 hrs. Then, the medium was replaced with 200 μl of DMSO and the absorbance was recorded at 570 nm ([12]). Each experiment was repeated three times and MTT assay was performed in 3 replicates for each experiments.…”

“…The conjugation of chemical or natural anticancer drugs to Fe 3 O 4 magnetite nanoparticles (MNPs) can lead to increse the uptake of anticancer agents by targeted tumor and improve their therapeutic index ([11]). For example, a significant difference in antiproliferation effect of a natural product, umbelliprenin, and its Fe 3 O 4 MNPs-conjugated form was observed in vitro model ([12]).…”

Sesquiterpene coumarins

“…The cytotoxicity of umbelliprenin (6) towards the fibrosarcoma cell line Wehi 164 was dose-dependent and showed good activity at 50 lg/mL (Shahverdi et al 2006). Khorramizadeh et al (2010) reported that the combination of Fe 3 O 4 magnetite nanoparticles with umbelliprenin (6) was much more cytotoxic against human fibrosarcoma cell line (HT-1080) (IC 50 = 9 lg/mL) than umbelliprenin (6) alone (IC 50 = 50 lg/mL). This 7-prenyloxycoumarin exhibited also anticoagulant activity (Rosselli et al 2007) as well as antileishmanial activities against promastigotes of Leishmania major .…”