Fabrication of docetaxel surfaced Fe3O4 magnetite nanoparticles and their cytotoxicity on 4 T1 breast cancer cells

Abstract

BackgroundIn the recent years, there is an increasing attention to the using of Fe3O4 magnetite nanoparticles (MNPs) as drug delivery systems. Application of this nanoparticles could profit advantages of nanomedicine to enhance biological activity of pharmaceutical ingredients.MethodsFe3O4 MNPs were synthesised by a chemical method and characterized by transmission electron microscopy and energy-dispersive spectroscopy techniques. In the next step, docetaxel-coated Fe3O4 MNPs were prepared, using percipitation method. The surface chemistry of docetaxel-coated Fe3O4 MNPs as well as their thermal decomposition characteristics were examined using fourier transform infrared spectroscopy and thermogravimetric analyzer equipment, respectively. The cytotoxicity assay was conducted on 4 T1 breast cancer carsinoma by MTT assay to evaluate the possible in vitro antiproliferative effects of docetaxel-coated Fe3O4 MNPs.ResultsDuring precipitation process, docetaxel molecules were precipitated on the surface of Fe3O4 MNPs by the ratio of 3:100 w/w which indicates that each milligram of coated Fe3O4 MNPs averagely contained 30 μg pure docetaxel compound. Docetaxel showed aniproliferative effects against mentioned cell line. The higestest concentartion of docetaxel (80 μg/ml) caused about 80% cell death. However, the results demostarted that much lower amounts of docetaxel will be needed in combination of Fe3O4 MNPs to produce the potent antiproliferative effect compared to docetaxel alone. Dose response cytotoxicity assay of docetaxel-coated Fe3O4 MNPs against 4 T1 breast cancer cells showed that lower amount of docetaxel (0.6 μg/ml) can exhibit higher cytotoxic effect against this cancer cell line (90% cell death).

Cytotoxicity and genotoxicity of iron oxide nanoparticles: An in vitro biosafety study

“…We observed that magnetite at concentrations above 100 mg/L induced a decrease in the percentage of viable cells, while at concentrations above 150 mg/L, magnetite treatments led to a pronounced LDH release in cultured human blood cells as compared to control values. Our results are in agreement with a previous study (Yazdi et al, 2012), which reported that magnetite NPs demonstrated strong cytotoxicity towards 4T1 breast cancer cells. The possible cytotoxic effects of magnetite have been reported by various researchers (Ding et al, 2010;Könczöl et al, 2011).…”

Nanoways to overcome docetaxel resistance in prostate cancer

“…Recent studies suggest that multi-layer magnetic nanoparticle system(s) has been highly encouraged due to their theranostic (therapy and imaging/diagnosis) applications in cancer (Yallapu et al, 2013b; Yallapu et al, 2010a; Yallapu et al, 2012b; Yallapu et al, 2011). Yazdi et al (Yazdi et al, 2012) synthesized a magnetic nanoparticle loaded with docetaxel which showed higher cytotoxicity than the free drug in cancer cells and this delivery system can be used to inhibit development of resistance in cancer cells. This formulation has shown to be effective even at very low concentration levels.…”