Ultraviolet Spectroscopy and Its Pharmaceutical Applications- A Brief Review

Abstract

Rapid and easy analytical methods are needed due to increasing number of multicomponent formulations, biotherapeutic products and samples of complex matrix in que. Number of Ultraviolet (UV) spectrophotometric methods used for these purpose. Different types of UV spectrometric methods developed on the basis of principle of additivity, absorbance difference, processing absorption spectra. The aim of this review is to present information on simultaneous equation method, difference spectrophotometry, derivative spectrophotometry, absorbance ratio spectra, derivative ratio spectra, successive ratio -derivative spectra, Q-absorbance ratio method, absorptivity factor method, dual wavelength method, absorption factor method, multivariate chemometric methods, and isosbestic point method. A brief summary on theories, mathematical background and some applications of these methods are presented here.

ATR-FTIR spectroscopy and spectroscopic imaging for the analysis of biopharmaceuticals

“…For example, mass spectrometry (MS), is usually focussed at the molecular level to monitor post translational modifications (PTMs), and for the identification of numerous proteins in a protein mixture, but it is costly to run [40]. UV spectroscopy is utilised in a variety of set ups to elucidate protein-protein interactions through monitoring changes in UV absorbance using delta absorbance (ΔAbs), but is used primarily for protein concentration measurements [41][42][43]. A recently revived technique, analytical ultracentrifugation, obtains high resolution data about molecule size of the sample in liquid state, allowing information to be collected about the aggregation status of the protein [44].…”

Different Ultraviolet Spectroscopic Methods: A Retrospective Study on Its Application From the Viewpoint of Analytical Chemistry

“…This method is applicable for a combination of drugs which obeys Beer's law and the absorbance ratio at two wavelengths is a constant value which is independent of the path length and concentration. This constant is termed as Q-value or Hafner's Quotient [7,8].…”

“…It is a modified method of simultaneous equation method. It is a simple, reproducible, sensitive, and economical method [7,8].…”

“…It is only applicable for the combination of drugs which are having the isoabsorptive point and which follows Beer's law [7,8].…”

Comparison of polystyrene versus cycloolefin microplates in absorbance measurements in the UV/VIS region of the spectrum

“…UV/VIS is a subclass of spectroscopy which uses visible light and adjacent near ultraviolet (UV) ranges for determining the concentrations and the characterization of dissolved substances (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11). This spectroscopy is usually conducted in quart glass cuvettes.…”