Ultraviolet Radiation–Induced Cataract: Age and Maximum Acceptable Dose

Dong, Xiaofang; Ayala, Marcelo; Löfgren, Stefan; Söderberg, Per G.

doi:10.1167/iovs.02-0541

Cited by 38 publications

(28 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Two previous studies explicitly investigated the age effect in UV cataractogenesis in the albino rat, using degree of cataract as the endpoint, and they showed a low tolerance to UV exposure in 3-and 6-week old rats, compared with older rats (52 weeks). 42,52 This contrasts to our current results on pigmented mouse where the macroscopic lens changes seemed to be more prominent in the old mice. The reason for this discrepancy is unclear, but likely the difference in the UV spectrum, the animal species, and the pigmentation of the animal may all play some roles.…”

Section: Discussioncontrasting

confidence: 99%

“…19,26,27 The relevance of PSSG as a good marker for protein thiol oxidation in the lens is further established with the findings that aging and the degree of opacity in human old or cataractous lenses are well corroborated with the loss in free GSH and the accumulation of PSSG. 40,41 Although UV radiation has been used successfully in the past for inducing light scattering or opacity in the animal lenses in vivo or in vitro, and the threshold of UVB for oxidative damage in lens epithelial cells or whole lens have been established, 42 little is known about the biochemical effects of animal aging in UV cataractogenesis, and the nature of the oxidation damage on the lens protein thiols or the role of thiol oxidation damage repair systems in lenses exposed to UV radiation. Considering the strong indications of solar UV radiation as a risk factor in age-related cataract, it is important to understand if aging compromises the protection mechanisms relevant to UV exposure.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Ultraviolet Radiation–Induced Cataract in Mice: The Effect of Age and the Potential Biochemical Mechanism

Zhang

Löfgren

et al. 2012

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

PURPOSE.To study the effect of age on the morphologic and biochemical alterations induced by in vivo exposure of ultraviolet radiation (UV).METHODS. Young and old C57BL/6 mice were exposed to broadband UVBþUVA and euthanized after 2 days. Another batch of UV-exposed young mice was monitored for changes after 1, 2, 4, and 8 days. Age-matched nonexposed mice served as controls. Lens changes were documented in vivo by slit-lamp biomicroscopy and dark field microscopy photographs ex vivo. Lens homogenates were analyzed for glutathione (GSH) level, and the activities of thioredoxin (Trx), thioltransferase (TTase), and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Glutathionylated lens proteins (PSSGs) were detected by immunoblotting using GSH antibody. Western blot analysis was also done for the expression levels of TTase and Trx.RESULTS. Both age groups developed epithelial and superficial anterior subcapsular cataract at 2 days postexposure. The lens GSH level and G3PD activity were decreased, and PSSGs were elevated in both age groups, but more prominent in the older mice. TTase and Trx activity and protein expression were elevated only in the young mice. Interestingly, lens TTase and Trx in the young mice showed a transient increase, peaking at 2 days after UV exposure and returning to baseline at day 8, corroborated by lens transparency.CONCLUSIONS. The lenses of old mice were more susceptible to UV radiation-induced cataract. The upregulated TTase and Trx likely provided oxidation damage repair in the young mice. (Invest Ophthalmol Vis Sci. 2012;53:7276-7285)

show abstract

Section: Discussioncontrasting

confidence: 99%

mentioning

confidence: 99%

Ultraviolet Radiation–Induced Cataract in Mice: The Effect of Age and the Potential Biochemical Mechanism

Zhang

Löfgren

et al. 2012

Invest. Ophthalmol. Vis. Sci.

View full text Add to dashboard Cite

show abstract

“…24 reported environmental influence such as near work, night lighting and ultraviolet exposure may be responsible for ageing of the crystalline lens and associated myopia. In contrast to population based studies from Australia 18 , Singapore 19 , and Indonesia 25 , the incidence of myopia was more in males than females in our study.…”

Section: Discussionmentioning

confidence: 99%

Incidence of Refractive Error and Amblyopia Among Young Adults - A Hospital Based Study

Narsani¹,

Jatoi²,

Maheshwari³

et al. 2012

Advances in Ophthalmology

View full text Add to dashboard Cite

“…Measurement of opacity of crystal lens proteins. Cataracts were graded on a 0 to ϩ3 scale by placing the lenses on a grid sheet, as described previously (18,19). The lens samples were photographed, and the images captured were analyzed using ImageJ (National Institutes of Health).…”

Section: Methodsmentioning

confidence: 99%

“…Cataractogenesis was determined based on the four phases of grading as described previously (18,19), and quantitative evaluation by ImageJ as described in "Materials and Methods." The development of cataracts, which was estimated by transparency, was enhanced in mice with diabetes compared to that in normal mice (pϭ0.04), whereas Asa administration inhibited the progression of cataracts to a nearly normal level (pϭ0.13) (Fig.…”

Section: Cataract Progressionmentioning

confidence: 99%

<i>Aphanothece sacrum</i> (Sur.) Okada Prevents Cataractogenesis in Type 1 Diabetic Mice

Matsuda

Sugawa

Shirakawa

et al. 2017

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

View full text Add to dashboard Cite

Summary Aphanothece sacrum (Sur.) Okada is a species of cyanobacteria found in Japan. Although it has been used in local cuisine in Kyushu, Japan, for 250 y, little is known about its beneficial effect as food. The daily intake of health beneficial phytochemicals is believed to be useful for preventing lifestyle-related diseases, such as diabetic cataracts. In this study, the inhibitory effect of freeze-dried A. sacrum (Asa) on the formation of diabetic cataracts (DCs) was evaluated. Type 1 diabetes was induced in mice using streptozotocin (STZ). The mice were divided into two groups: one was fed a normal diet (DM-control group) and the other was fed a diet containing 1% Asa (DM-Asa group). During the study, changes in blood glucose levels and the amount of food and water consumed were measured. After 3 mo, the amount of N ε -(carboxymethyl)lysine (CML), an oxidative stress marker, in the lens was measured using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Although the blood glucose levels (pϭ0.91) and food consumption did not significantly change in any group, the oral administration of Asa tended to suppress CML accumulation (pϭ0.15) and significantly inhibited the progression of cataractogenesis in the diabetic lens compared with that reported for the normal diet (pϭ0.009). These results suggested that the daily intake of A. sacrum prevents the pathogenesis of cataracts, and indicated that may reduce the number of DC patients.

show abstract

Ultraviolet Radiation–Induced Cataract: Age and Maximum Acceptable Dose

Abstract: Young rats were more sensitive to UVR-B than old ones. Age should be considered when estimating the risk for UVR-B-induced cataract.

Cited by 38 publications

References 24 publications

Ultraviolet Radiation–Induced Cataract in Mice: The Effect of Age and the Potential Biochemical Mechanism

Ultraviolet Radiation–Induced Cataract in Mice: The Effect of Age and the Potential Biochemical Mechanism

Incidence of Refractive Error and Amblyopia Among Young Adults - A Hospital Based Study

<i>Aphanothece sacrum</i> (Sur.) Okada Prevents Cataractogenesis in Type 1 Diabetic Mice

Contact Info

Product

Resources

About