Ultraviolet B-Induced DNA Damage in Human Epidermis Is Modified by the Antioxidants Ascorbic Acid and D-α-Tocopherol

Placzek, Marianne; Gaube, S; Kerkmann, Urs; Gilbertz, Klaus-Peter; Herzinger, Thomas; Haen, Ekkehard; Przybilla, Bernhard

doi:10.1111/j.0022-202x.2004.23560.x

Cited by 110 publications

(73 citation statements)

References 29 publications

(27 reference statements)

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“…This may be due to a relatively greater number of photons available for excitation of the molecule at lower concentration compared to that at a higher concentration. The AH 2 concentrations of creams used in this study are within the range (1-15%) reported by previous workers for topical applications to skin (13)(14)(15).…”

Section: Resultssupporting

confidence: 83%

“…It is sensitive to air and light (2,3) and is degraded by chemical (4) and photochemical oxidation (5)(6)(7)(8)(9)(10). Ascorbic acid is an ingredient of antiaging cosmetic products (11)(12)(13)(14)(15) and exerts several functions on the skin as collagen synthesis, depigmentation, and antioxidant activity (16). As an antioxidant it protects skin by neutralizing reactive oxygen species generated on exposure to sunlight (17).…”

Section: Introductionmentioning

confidence: 99%

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Photostability and Interaction of Ascorbic Acid in Cream Formulations

et al. 2011

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Abstract. The kinetics of photolysis of ascorbic acid in cream formulations on UV irradiation has been studied using a specific spectrophotometric method with a reproducibility of ±5%. The apparent first-order rate constants (k obs ) for the photolysis of ascorbic acid in creams have been determined. The photoproducts formed in the cream formulations include dehydroascorbic acid and 2,3-diketogulonic acid. The photolysis of ascorbic acid appears to be affected by the concentration of active ingredient, pH, and viscosity of the medium and formulation characteristics. The study indicates that the ionized state and redox potentials of ascorbic acid are important factors in the photostability of the vitamin in cream formulations. The viscosity of the humectant present in the creams appears to influence the photostability of ascorbic acid. The results show that the physical stability of the creams is an important factor in the stabilization of the vitamin. In the cream formulations stored in the dark, ascorbic acid undergoes aerobic oxidation and the degradation is affected by similar factors as indicated in the photolysis reactions. The rate of oxidative degradation in the dark is about seventy times slower than that observed in the presence of light.

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Section: Resultssupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Photostability and Interaction of Ascorbic Acid in Cream Formulations

et al. 2011

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“…Among them, L-ascorbic acid and DL-α-tocopherol have been extensively studied. Specifically, L-ascorbic acid has been shown to reduce UV-induced photodamage in a porcine skin model (27) and human cells (28). Notably, Lin et al have developed a topical formulation consisting of 15% L-ascorbic acid combined with 1% DL-α-tocopherol (17).…”

Section: Discussionmentioning

confidence: 99%

“…Notably, Lin et al have developed a topical formulation consisting of 15% L-ascorbic acid combined with 1% DL-α-tocopherol (17). When DL-α-tocopherol neutralizes oxidative stress in lipids, its oxidation product can be regenerated by L-ascorbic acid (28)(29)(30)(31)(32)(33)(34)(35)(36). L-(+)-ergothioneine is a potent, natural sulfur-containing antioxidant that can protect biological macromolecules against copper-dependent oxidative damage (37) and enhance DNA repair in UV-irradiated cells (38).…”

Section: Discussionmentioning

confidence: 99%

Protective effect of trehalose-loaded liposomes against UVB-induced photodamage in human keratinocytes

Emanuele¹,

Bertona²,

Sanchís-Gomar

et al. 2014

Biomedical Reports

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Abstract. Trehalose, a naturally occurring non-reducing disaccharide, is known to act as a major protein stabilizer that can reduce ultraviolet B (UVB)-induced corneal damage when topically applied to the eye. However, due to the low skin permeability of trehalose, which makes the development of topical formulations difficult, its use as a skin photoprotective agent has been limited. Previous findings demonstrated that liposomes may significantly improve the intracellular delivery of trehalose. Therefore, the present study aimed to assess the protective effects of trehalose-loaded liposomes against UVB-induced photodamage using the immortalized human keratinocyte cell line, HaCaT. The effects were also compared to those of the common skin photoprotective compounds, including L-carnosine, L-(+)-ergothioneine, L-ascorbic acid and DL-α-tocopherol. The levels of cyclobutane pyrimidine dimers, 8-hydroxy-2'-deoxyguanosine and protein carbonylation in HaCaT cells were used as biological markers of UVB-induced damage. Compared to other compounds, trehalose-loaded liposomes showed the highest efficacy in reducing the levels of the three markers following UVB irradiation of HaCaT cells (all P<0.001 when compared to each of the four other photoprotective compounds). Therefore, these findings indicate that there may be a clinical application for trehalose-loaded liposomes, and further studies should be performed to assess the potential usefulness in skin photoprotection and the prevention of non-melanoma skin cancer.

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“…If this DNA damage is unrepaired in the cells of exposed human skin areas, cellular functions are interfered, 7) resulting in cell death, precancerous lesions in the skin, 8) or skin cancer. 9) In our laboratory, the molecular mechanisms underlying the UVC resistance of cultured human cells have been examined. We found that heat shock protein 27 (HSP27) is involved in the UVC resistance of human cells 10) and identified annexin II as an HSP27-interacting protein, that is involved in the protection of human cells against the lethal effects of UVC.…”

mentioning

confidence: 99%