Ultrastructure ofAspergillus fumigatus conidia development and maturation

Ghiorse, William C.; Edwards, Mercedes R.

doi:10.1007/bf01279672

Cited by 33 publications

(13 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also at this time that the amount of restrictocin detectable is greatest. In many species of Aspergillus, the first conidia emerge through the outer wall of the phialides, forming fresh cell wall within collarettes at the tip of the phialides (Trinci et al, 1968;Tsukahara, 1970;Oliver, 1972;Ghorse & Edwards, 1973;Hanlin, 1976). Our results suggest that a similar mechanism operates in A .…”

Section: Discussionsupporting

confidence: 49%

Production and localization of restrictocin in Aspergillus restrictus

Brandhorst

Kenealy²

1992

Journal of General Microbiology

View full text Add to dashboard Cite

The production and secretion of restrictwin (a cytotoxin that cleaves ribosomal RNA) by cultures of the fungus Aspergillus restrictus was investigated. Previous studies have indicated that restrictwin production in liquid culture coincides with the appearance of differentiated cell structures. A study of the correlation between the appearance of differentiated structures and restrictwin production was conducted with A. restrictus grown on agar medium. Restrictwin was found to be associated with the cell mass of the agar-grown culture (in contrast to liquid cultures), and was first observed when aerial hyphae emerged. Restrictwin levels increased until the time of conidiation, after which they fell off sharply. No restrictwin could be found in the agar medium. The presence of restrictwin upon and within various cell structures was determined by immunofluorescent laser microscopy. This study showed that restrictwin became localized to the conidiophores and phialides during the process of conidiation. Prior to this, restrictwin was found within the hyphae in localized concentrations that may correspond to secretory vesicles.

show abstract

Section: Discussionsupporting

confidence: 49%

Production and localization of restrictocin in Aspergillus restrictus

Brandhorst

Kenealy²

1992

Journal of General Microbiology

View full text Add to dashboard Cite

show abstract

“…Rodlets of the type described here were first seen on spores of various fungi (Sassen et al, 1967;Hess and Stocks, 1969;Bronchart and Demoulin, 1971;Cole, 1971Cole, , 1973Ghiorse and Edwards, 1973;Hashimoto et al, 1976;Beever and Dempsey, 1978;Cole and Samson, 1979). They were found to consist of protein (Hashimoto et al, 1976;Beever, 1979).…”

Section: Discussionmentioning

confidence: 85%

Interfacial Self-Assembly of a Fungal Hydrophobin into a Hydrophobic Rodlet Layer.

1993

View full text Add to dashboard Cite

The Sc3p hydrophobin of the basidiomycete Schizophyllum commune is a small hydrophobic protein (100 to 101 amino acids) containing eight cysteine residues. Large amounts of the protein are excreted into the culture medium as monomers, but in the walls of aerial hyphae, the protein is present as an SDS-insoluble complex. In this study, we show that the Sc3p hydrophobin spontaneously assembles into an SDS-insoluble protein membrane on the surface of gas bubbles or when dried down on a hydrophilic surface. Electron microscopy of the assembled hydrophobin shows a surface consisting of rodlets spaced 10 nm apart, which is similar to those rodlets seen on the surface of aerial hyphae. When the purified Sc3p hydrophobin assembles on a hydrophilic surface, a surface is exposed with high hydrophobicity, similar to that of aerial hyphae. The rodlet layer, assembled in vivo and in vitro, can be disassembled by dissolution in trifluoroacetic acid and, after removal of the acid, reassembled into a rodlet layer. We propose, therefore, that the hydrophobic rodlet layer on aerial hyphae arises by interfacial self-assembly of Sc3p hydrophobin monomers, involving noncovalent interactions only. Submerged hyphae merely excrete monomers because these hyphae are not exposed to a water-air interface. The generally observed rodlet layers on fungal spores may arise in a similar way.

show abstract

“…Rodlets were first observed on the outer surface of freezeetched spores from Penicillium (Sassen et al, 1967;Hess et al, 1968), Aspergillus (Hess et al, 1969;Ghiorse and Edwards, 1973), Oidiodendron truncatum and Geotrichum candidum (Cole, 1973). However, the first detailed images and evidence of their function were not provided until the appearance of three papers in 1978-9 (Beever and Dempsey, 1978; Beever et al, 1979; Dempsey and Beever, 1979).…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 94%