Production and localization of restrictocin in Aspergillus restrictus

Brandhorst, T. Tristan; Kenealy, William R.

doi:10.1099/00221287-138-7-1429

Cited by 27 publications

(19 citation statements)

References 21 publications

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“…These results showed a significant decrease in feeding on the first of these two fungi when conidia were starting to develop, while no change in the insect behavior was observed against maturing A. nidulans. The period of not consumption was coincident with a dramatic build up of restrictocin upon the surfaces of conidia and phialides (Brandhorst and Kenealy, 1992) suggesting that it could deter insects from feeding until conidia were fully formed. Once the spores were mature, the ribotoxin levels would decrease allowing insects to carry spores to new locations for spreading the fungus.…”

Section: Introductionmentioning

confidence: 99%

Fungal extracellular ribotoxins as insecticidal agents

Olombrada¹,

Herrero-Galán²,

Tello³

et al. 2013

Insect Biochemistry and Molecular Biology

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Fungal ribotoxins were discovered almost 50 years ago as extracellular ribonucleases (RNases) with antitumoral properties. However, the biological function of these toxic proteins has remained elusive. The discovery of the ribotoxin HtA, produced by the invertebrates pathogen H. thompsonii, revived the old proposal that insecticidal activity would be their long searched function. Unfortunately, HtA is rather singular among all ribotoxins known in terms of sequence and structure similarities. Thus, it was intriguing to answer the question of whether HtA is just an exception or, on the contrary, the paradigmatic example of the ribotoxins function. The work presented uses HtA and -sarcin, the most representative member of the ribotoxins family, to show their strong toxic action against insect larvae and cells.

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Section: Introductionmentioning

confidence: 99%

Fungal extracellular ribotoxins as insecticidal agents

Olombrada¹,

Herrero-Galán²,

Tello³

et al. 2013

Insect Biochemistry and Molecular Biology

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“…The accompanying paper (Brandhorst & Kenealy, 1992) reports experiments on the cellular locat ion of restric tocin .…”

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confidence: 99%

Regulation of restrictocin production in Aspergillus restrictus

Yang¹,

Kenealy²

1992

Journal of General Microbiology

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The production of restrictwin (a cytotoxin that specifically cleaves ribosomal RNA) by cultures of Aspergiffus restrictus grown in liquid medium was investigated. The function of restrictwin, the method of its accumulation and the mode of resistance to restrictwin in A. restrictus are unknown. Previous studies have indicated that restrictwin accumulates in the medium with culture age. These observations have been extended in this study by cloning the cDNA of the res gene and using this cDNA clone to probe the onset of messenger RNA synthesis in the cells. The results of the Northern analysis were compared to the production and accumulation of restrictwin and morphological differentiation of the cells in culture. Restrictwin was found in the medium at the same time that mRNA was detected in the cells. This suggests that the leader sequence encoded by the cDNA provides an efficient secretion system for the protein. Both the protein and the mRNA were detected coincident with the formation of differentiated cell structures. These structures develop into conidiophores with one layer of sterigmata and conidia forming from the sterigmata. These results suggest that restrictwin is either involved in the process of conidiation or is coordinately regulated with differentiation leading to conidiation.

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“…Intemalisation of this protein can be also achieved by the construction of immunoconjugates, prepared by the attachment of the ribotoxin to monoclonal antibodies specific of receptors present on the surface of the target cells (Conde et al, 1989;Wawrzynczak et al, 1991). The occurrence of this ribosome inactivating protein in the course of an Aspergillus infection and its presence on the conidial surface has prompted several groups to consider this molecule as a potential virulence factor in aspergillosis Arruda et al, 1992;Brandhorst and Kenealy, 1992).…”

Section: Exoantigens and Virulencementioning

confidence: 99%