Ultrastructure of the secretory epithelium, nerve fibers, and capillaries in the mouse sweat gland

Quick, Donald C.; Kennedy, William R.; Yoon, Kwon S.

doi:10.1002/ar.1092080404

Cited by 25 publications

(19 citation statements)

References 25 publications

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“…There is a well-developed basement membrane in the coiled portion of the duct within the dermis, and the secretory loops could be distinguished from the ductal loops based on cell morphology (Fig 20-26). Similar to the findings of others, 28 only light cells appeared to be present in the secretory portion of the gland based on PAS reaction (Fig 27). Cells of the secretory coil therefore appeared as a distinct cuboidal monolayer sitting the basement membrane in most sections (Fig 27).…”

Section: Resultssupporting

confidence: 90%

Development, Structure, and Keratin Expression in C57BL/6J Mouse Eccrine Glands

et al. 2011

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Eccrine sweat glands in the mouse are found only on the footpads and, when mature, resemble human eccrine glands. Eccrine gland anlagen were first apparent at 16.5 days postconception (DPC) in mouse embryos as small accumulations of cells in the mesenchymal tissue beneath the developing epidermis resembling hair follicle placodes. These cells extended into the dermis where significant cell organization, duct development, and evidence of the acrosyringium were observed in 6-to 7-postpartum day (PPD) mice. Mouse-specific keratin 1 (K1) and 10 (K10) expression was confined to the strata spinosum and granulosum. In 16.5 and 18.5 DPC embryos, K14 and K17 were both expressed in the stratum basale and diffusely in the gland anlagen. K5 expression closely mimicked K17 throughout gland development. K6 expression was not observed in the developing glands of the embryo but was apparent in the luminal cell layer of the duct by 6 to 7 PPD. By 21 PPD, the gland apertures appeared as depressions in the surface surrounded by cornified squames, and the footpad surface lacked the organized ridge and crease system seen in human fingers. These data serve as a valuable reference for investigators who use genetically engineered mice for skin research.

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Section: Resultssupporting

confidence: 90%

Development, Structure, and Keratin Expression in C57BL/6J Mouse Eccrine Glands

et al. 2011

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“…Likewise, the staining pattern for injected agalsidase alfa does not follow the relative organ blood flows or the presence or absence of fenestrated capillaries in various organs. Fenestrated capillaries are present in the liver, the adrenal cortex, and renal tubuli that showed marked enzyme uptake, but they are also present in endocrine tissue, pancreas and sweat glands that showed no detectable immunoreativity [24,25].…”

Section: Discussionmentioning

confidence: 99%

Cellular and tissue distribution of intravenously administered agalsidase alfa

Murray

Anver

Kennedy

et al. 2007

Molecular Genetics and Metabolism

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Abstractα-Galactosidase A is the lysosomal hydrolase that is deficient in patients with Fabry disease. Intravenous infusion of agalsidase alfa, a preparation of α-galactosidase A, is used for enzyme replacement therapy (ERT) in patients with Fabry disease. Although ERT appears to show some beneficial effects, most patients show only a modest response. We investigated using immunohistochemistry the relative tissue and cellular distribution of agalsidase alfa after a single intravenous injection in a mouse knockout model of Fabry disease. Specific immunostaining for agalsidase alfa was found only in liver, kidney, heart, testes, adrenal gland, spleen and bone marrow. There was no difference in distribution of the infused enzyme distribution among tissues sampled 4, 24, and 48 hours post-injection. The intracellular localization of immunopositivity varied considerably between organs with vascular endothelium being the most commonly positive site. α-Galactosidase A specific activity in tissue homogenates matched the relative extent of agalsidase alfa immunostaining distribution in the same organs. We conclude that intravenously injected agalsidase alfa has a very heterogeneous systemic distribution using an immunostaining technique.

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“…As it became clear that the mouse and rat palmar and plantar glands were structurally different from the simian and human eccrine glands, their usage, at this time, as a human gland model declined.…”

Section: Isolated Glandsmentioning

confidence: 99%

The evolution of eccrine sweat gland research towards developing a model for human sweat gland function

Bovell

2018

Experimental Dermatology

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For several decades now, researchers, professional bodies, governments, and journals such as the journal of Experimental Dermatology have worked to reduce the number of animals used in experimentation. This review centres on investigations into how human sweat glands produce sweat and how that research has evolved over the years. It is hoped that this review will show that as methodologies advanced, sweat gland research has come to rely less and less on a variety of animal models as investigative tools and information is being primarily obtained through human and mouse material, with a view to further reductions in using animal models.

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Ultrastructure of the secretory epithelium, nerve fibers, and capillaries in the mouse sweat gland

Cited by 25 publications

References 25 publications

Development, Structure, and Keratin Expression in C57BL/6J Mouse Eccrine Glands

Development, Structure, and Keratin Expression in C57BL/6J Mouse Eccrine Glands

Cellular and tissue distribution of intravenously administered agalsidase alfa

The evolution of eccrine sweat gland research towards developing a model for human sweat gland function

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