Ultrastructure of the Miracidium of Echinostoma paraensei Lie and Basch, 1967 (Trematoda, Echinostomatidae)

Pinheiro, Jairo; Maldonado, Arnaldo; Attias, Márcia; Lanfredi, R. M.

doi:10.1007/s00436-005-1458-8

Cited by 21 publications

(8 citation statements)

References 22 publications

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“…By using an approach that has been previously applied to S. mansoni adult worms and cercariae, we examined the distribution of filamentous actin in eggs, miracidia and primary sporocysts. Flame cells are thought to be protonephridial elements involved in the excretion process by S. mansoni forms and can also be found in other Trematoda (Pan, 1980 ;Pinheiro et al 2005 ;Rohde and Watson, 1992 ;Rollinson et al 1987). Already in that form, by careful examination of sequential optical sections, it was possible to observe previously undescribed actinrich tubules.…”

Section: Discussionmentioning

confidence: 99%

The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst

et al. 2006

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Schistosoma mansoni eggs, miracidia and primary sporocysts were labelled with phalloidin-rhodamine to visualize filamentous actin structures. Analysis of these forms by confocal fluorescence microscopy revealed the presence of previously well-defined circular and longitudinal muscle layers. Besides these muscular layers that sustain and provide motility to these parasite forms, we found in these 3 consecutive developmental stages of the parasite previously unidentified actin-rich tubular structures. In the 3 forms, 4 actin-rich tubules could be observed by optical sectioning underneath the well-developed muscle layers. The tubules appear in pairs, transversal to the length of the parasite, and located towards the extremities. By using an anti-flame cell specific antibody we confirmed that the tubules co-localize with flame cells and also determined that the tubule core is filled with microtubules. The additional presence of myosin in these tubules strongly suggests that they are contractile structures.

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Section: Discussionmentioning

confidence: 99%

The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst

et al. 2006

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“…The dried material was mounted on metallic stubs, covered with double-face carbon tape and golden coated (Balzers). The samples were observed under a Jeol JSM 5310 scanning electron microscope, using 20 kV, and the images were digitalized through the SEMAfore software (Pinheiro et al, 2005).…”

Section: Processing For Scanning Electron Microscope (Sem)mentioning

confidence: 99%

Nematode cysts and larvae found in Achatina fulica Bowdich, 1822

Franco-Acuña

Pinheiro

Torres

et al. 2009

Journal of Invertebrate Pathology

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“…Also, this is the fi rst report of the ultrastructure of the eggshell, which is thicker in the anterior and posterior ends and thinner in the middle/lateral region. Also, there are no specializations such as the knob described at the posterior end of the eggshell in Echinostoma paraensei (Pinheiro et al, 2004a;b). This characteristic has not been shown before.…”

Section: Discussionmentioning

confidence: 78%

Additional study of the morphology of eggs and miracidia of Eurytrema coelomaticum (Trematoda)

et al. 2015

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SummaryThe morphology of the eggs and miracidia of Eurytrema coelomaticum was analyzed. The eggshell presented different composition according to the maturation stage of the egg, as seen by distinct birefringence after polarized light microscopic analysis. Detailed morphology of the brownish eggs, measuring 44.97±3.83 (38.67 -51.40) μm in length and 30. 71±2.54 (25.79 -34.47) μm in width, and the different electrondensities of the inner, middle and outer layers of the eggs were determined using light (bright fi eld and DIC) and electronic microscopy (scanning and transmission). The morphometry of the eggs is in accordance to those measures presented in previous studies. The miracidia were observed using light microscopy, inside and outside the egg. It was possible to observe the terebratorium at the anterior end of the body, the ciliated epidermal plates, placed according the formula E=2E 1 + 2E 2 , and the interepidermal ridge, but eyespots were not observed. The excretory vesicle was laterally placed in the posterior middle of the body and the germ balls were observed. The miracidium inside the egg was observed by routine techniques, but the miracidium was not well preserved. Thus, the eggs were processed using high pressure-freeze substitution technique and reasonable preservation was achieved. The cilia covering the larval body, with a typical ciliary organization, the terebratorium with folds and the junction of the epidermal ciliated cell with the larval body were seen. For the fi rst time detailed information on the eggs and miracidia of E. coelomaticum is given, which can be the basis for new studies.

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Ultrastructure of the Miracidium of Echinostoma paraensei Lie and Basch, 1967 (Trematoda, Echinostomatidae)

Cited by 21 publications

References 22 publications

The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst

The distribution of motor proteins in the muscles and flame cells of the Schistosoma mansoni miracidium and primary sporocyst

Nematode cysts and larvae found in Achatina fulica Bowdich, 1822

Additional study of the morphology of eggs and miracidia of Eurytrema coelomaticum (Trematoda)

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