Ultrastructure of the Cyst Wall of Sarcocystis Sp. In Roe Deer

Santini, Samuele; Mancianti, Francesca; Nigro, Marco; Poli, Alessandro

doi:10.7589/0090-3558-33.4.853

Cited by 15 publications

(8 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…hofmanni) and the Sarcocystis sp. discovered by Schramlova and Blažek in 1978 and later described in more detail by TEM by Santini et al (1997), were not found in the present study. The absence of these three species might be due to the fact that only a few animals from a limited geographical area were examined rather than an absence of these species in roe deer in Norway.…”

Section: S Oviformis In Skeletal Musclecontrasting

confidence: 54%

“…has been recognized by TEM in several studies, first by Schramlova and Blažek in 1978 (Fig. 1) and later by Entzeroth (1982) (Type 4) and Santini et al (1997). This species has sarcocysts with a similar morphology to those of Sarcocystis grueneri in reindeer (Gjerde 1985b), Sarcocystis cevicanis/Sarcocystis wapiti of red deer/wapiti (Hernández et al 1981;Speer and Dubey 1982), a Sarcocystis sp.…”

Section: Introductionmentioning

confidence: 90%

See 1 more Smart Citation

Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species

Dahlgren

Gjerde

2008

Parasitol Res

View full text Add to dashboard Cite

Fresh muscle tissue from six roe deer from Southeastern Norway was examined for sarcocysts. Cysts were excised and morphologically classified by light microscopy, and some cysts were further examined by scanning electron microscopy or DNA amplification and sequencing of the small subunit (ssu) rRNA gene. Two Sarcocystis species, Sarcocystis gracilis and Sarcocystis oviformis n. sp., were found and described by morphological and molecular methods. S. gracilis was found in all animals, whereas S. oviformis was found in only one roe deer. Polymerase chain reaction identification was necessary for definitive species identification, since cysts of S. gracilis varied in surface structure and since cysts of both S. gracilis and S. oviformis were morphologically indistinguishable from sarcocysts in other cervidae. Phylogenetic analyses based on complete ssu rRNA gene sequences revealed a close relationship between S. gracilis and other canine-transmitted Sarcocystis species, whereas S. oviformis formed a well-supported group with Sarcocystis hardangeri of reindeer and Sarcocystis ovalis of moose.

show abstract

Section: S Oviformis In Skeletal Musclecontrasting

confidence: 54%

Section: Introductionmentioning

confidence: 90%

Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species

Dahlgren

Gjerde

2008

Parasitol Res

View full text Add to dashboard Cite

show abstract

“…1 of their paper, then as Type 4 cysts by Entzeroth (1982) and finally as an unnamed Sarcocystis sp. described in detail by Santini et al (1997). By TEM, this species is indistinguishable from S. grueneri of reindeer (Gjerde 1985), Sarcocystis cervicanis/Sarcocystis wapiti of red deer/wapiti (Hernández et al 1981;Speer and Dubey 1982), a Sarcocystis sp.…”

Section: S Cf Gruenerimentioning

confidence: 88%

Morphological and molecular characterization and phylogenetic placement of Sarcocystis capreolicanis and Sarcocystis silva n. sp. from roe deer (Capreolus capreolus) in Norway

Gjerde

2011

Parasitol Res

View full text Add to dashboard Cite

Sarcocysts were isolated from the muscle tissue of three roe deer from southeastern Norway and examined by light microscopy, scanning electron microscopy and/or sequencing of the small subunit ribosomal RNA (ssu rRNA) gene. By light microscopy, four sarcocyst types were found, including those of Sarcocystis gracilis and Sarcocystis oviformis, which had been characterized previously. The third cyst type had about 10 μm long, flexible, hair-like surface protrusions, consistent with those of Sarcocystis capreolicanis, and differed genetically from other known species. The name S. capreolicanis was therefore assigned to this sequence type. The fourth cyst type had densely packed, upright, finger-like surface protrusions, about 8 μm long, and was morphologically similar to an unnamed Sarcocystis sp. reported from roe deer in other countries, and identical at the ssu rRNA gene to Sarcocystis sp. Type D previously reported from moose. This species was assigned the new name Sarcocystis silva. Both S. capreolicanis and S. silva displayed considerable intraspecific variation at the ssu rRNA gene. In phylogenetic analyses based on ssu rRNA gene sequences, S. capreolicanis grouped together with other canine-transmitted Sarcocystis species, whereas S. silva was most closely related to Sarcocystis rangiferi and Sarcocystis tarandi of reindeer. Roe deer muscles containing numerous cysts of S. gracilis were fed to a silver fox (Vulpes vulpes) and a blue fox (Vulpes lagopus), both of which started shedding Sarcocystis sporocysts in their faeces 9 days later, and harboured numerous oocysts, measuring about 20 × 15 μm, in their intestinal mucosa upon euthanasia 14 days post-inoculation. DNA derived from these oocysts was amplified and sequenced at the ssu rRNA gene and belonged to S. gracilis, confirming for the first time by molecular methods that foxes are definitive hosts for this species.

show abstract

“…The fourth unnamed Sarcocystis spp. that was discovered in roe deer with an aid of TEM (Schramlová and Blažek 1978; Entzeroth 1982; Santini et al 1997) resembled sarcocysts of Sarcocystis grueneri found in reindeer (Gjerde 1985), Sarcocystis cevicanis in red deer, and Sarcocystis wapiti in wapiti (Hernandez et al 1981; Speer and Dubey 1982). The last Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 98%

“…hofmanni , were identified in European roe deer using light microscopy (LM), transmission electron microscopy (TEM), and combination thereof (Erber et al 1978; Entzeroth 1981, 1982; Enzeroth 1985; Sedlaczek and Wesemeier 1995; Santini et al 1997; Stolte et al 1996, 1998). The fourth unnamed Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and cox1 sequence analysis

2014

View full text Add to dashboard Cite

Sarcocysts from four Polish roe deer were collected and examined by light microscopy, small subunit ribosomal RNA (ssu rRNA), and the subunit I of cytochrome oxidase (cox1) sequence analysis. This resulted in identification of Sarcocystis gracilis, Sarcocystis oviformis, and Sarcocystis silva. However, we were unable to detect Sarcocystis capreolicanis, the fourth Sarcocystis species found previously in Norwegian roe deer. Polish sarcocysts isolated from various tissues differed in terms of their shape and size and were larger than the respective Norwegian isolates. Analysis of ssu rRNA gene revealed the lack of differences between Sarcocystis isolates belonging to one species and a very low degree of genetic diversity between Polish and Norwegian sarcocysts, ranging from 0.1 % for Sarcocystis gracilis and Sarcocystis oviformis to 0.44 % for Sarcocystis silva. Contrary to the results of the ssu rRNA analysis, small intraspecies differences in cox1 sequences were found among Polish Sarcocystis gracilis and Sarcocystis silva isolates. The comparison of Polish and Norwegian cox1 sequences representing the same Sarcocystis species revealed similar degree of sequence identity, namely 99.72 % for Sarcocystis gracilis, 98.76 % for Sarcocystis silva, and 99.85 % for Sarcocystis oviformis. Phylogenetic reconstruction and genetic population analyses showed an unexpected high degree of identity between Polish and Norwegian isolates. Moreover, cox1 gene sequences turned out to be more accurate than ssu rRNA when used to reveal phylogenetic relationships among closely related species. The results of our study revealed that the same Sarcocystis species isolated from the same hosts living in different geographic regions show a very high level of genetic similarity.Electronic supplementary materialThe online version of this article (doi:10.1007/s00436-014-3966-x) contains supplementary material, which is available to authorized users.

show abstract

Ultrastructure of the Cyst Wall of Sarcocystis Sp. In Roe Deer

Cited by 15 publications

References 2 publications

Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species

Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species

Morphological and molecular characterization and phylogenetic placement of Sarcocystis capreolicanis and Sarcocystis silva n. sp. from roe deer (Capreolus capreolus) in Norway

Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and cox1 sequence analysis

Contact Info

Product

Resources

About