Morphological and molecular characterization and phylogenetic placement of Sarcocystis capreolicanis and Sarcocystis silva n. sp. from roe deer (Capreolus capreolus) in Norway

Gjerde, Bjørn

doi:10.1007/s00436-011-2619-6

Cited by 40 publications

(33 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comparing 1500 aligned positions of 18S rRNA sequences, the highest identity of 92.2% for S. aucheniae Australia and 92.3% for South American isolates J2, J3, and J5 was exhibited with Sarcocystis sinensis, while the lowest identity of 86.7% for S. aucheniae Australia and 86.8% for South American isolates J2, J3, J5 was observed for S. singaporensis (data not shown). Notably, a considerably larger inter-species variation of the 18S rRNA gene is observed in Sarcocystidae (7.8-13.2%), as compared to some other apicomplexan pathogens as for example piroplasmids (0.3-8.2%) (Schnittger et al, 2003;Dahlgren and Gjerde, 2007;Gjerde, 2012;Whipps et al, 2012). The Bayesian tree shown in Fig.…”

Section: Resultsmentioning

confidence: 95%

Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas

Carletti¹,

Martin²,

Romero³

et al. 2013

Veterinary Parasitology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 95%

Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas

Carletti¹,

Martin²,

Romero³

et al. 2013

Veterinary Parasitology

View full text Add to dashboard Cite

“…(Tenter et al 1994; Stojecki et al 2012). Amplification of DNA and sequencing of the ssu rRNA gene from sarcocyst found in roe deer from Norway confirmed the presence of two Sarcocystis spp., the already described S. gracilis and S. capreolicanis , and revealed the existence of two new species, Sarcocystis oviformis (Dahlgren and Gjerde 2009) and Sarcocystis silva (Gjerde 2012). …”

Section: Introductionmentioning

confidence: 77%

“…Apart from domestic ruminants (Tenter 1995), Sarcocystis spp. were found in several species of cervids, such as reindeer (Dahlgren and Gjerde 2007, Dahlgren et al 2007, 2008), red deer (Dahlgren and Gjerde 2010), and roe deer (Gjerde 2012). …”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and cox1 sequence analysis

2014

View full text Add to dashboard Cite

Sarcocysts from four Polish roe deer were collected and examined by light microscopy, small subunit ribosomal RNA (ssu rRNA), and the subunit I of cytochrome oxidase (cox1) sequence analysis. This resulted in identification of Sarcocystis gracilis, Sarcocystis oviformis, and Sarcocystis silva. However, we were unable to detect Sarcocystis capreolicanis, the fourth Sarcocystis species found previously in Norwegian roe deer. Polish sarcocysts isolated from various tissues differed in terms of their shape and size and were larger than the respective Norwegian isolates. Analysis of ssu rRNA gene revealed the lack of differences between Sarcocystis isolates belonging to one species and a very low degree of genetic diversity between Polish and Norwegian sarcocysts, ranging from 0.1 % for Sarcocystis gracilis and Sarcocystis oviformis to 0.44 % for Sarcocystis silva. Contrary to the results of the ssu rRNA analysis, small intraspecies differences in cox1 sequences were found among Polish Sarcocystis gracilis and Sarcocystis silva isolates. The comparison of Polish and Norwegian cox1 sequences representing the same Sarcocystis species revealed similar degree of sequence identity, namely 99.72 % for Sarcocystis gracilis, 98.76 % for Sarcocystis silva, and 99.85 % for Sarcocystis oviformis. Phylogenetic reconstruction and genetic population analyses showed an unexpected high degree of identity between Polish and Norwegian isolates. Moreover, cox1 gene sequences turned out to be more accurate than ssu rRNA when used to reveal phylogenetic relationships among closely related species. The results of our study revealed that the same Sarcocystis species isolated from the same hosts living in different geographic regions show a very high level of genetic similarity.Electronic supplementary materialThe online version of this article (doi:10.1007/s00436-014-3966-x) contains supplementary material, which is available to authorized users.

show abstract

“…of Sika deer in Japan in an unpublished study but documented through 12 sequences deposited in GenBank in 2006 (AB251926, AB257085, AB257086, AB257154-AB257162). Inter-and intra-isolate variation in the 18S rRNA gene was later found in Sarcocystis scandinavica in moose (Dahlgren and Gjerde 2008); in Sarcocystis tarandi and Sarcocystis rangiferi in reindeer and in the two similar species in red deer (Dahlgren and Gjerde 2010), which have since been re-described as two separate species, Sarcocystis elongata and S. truncata, respectively (Gjerde 2014a); in Sarcocystis capreolicanis and Sarcocystis silva in roe deer (Gjerde 2012); and in Sarcocystis taeniata in moose (Gjerde 2014b). In these species from cervid intermediate hosts, the maximum intraspecific sequence divergence, when comparing the near full-length gene, has varied between 0.6 and 1.9 %.…”

Section: Discussionmentioning

confidence: 96%

Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt

2015

Self Cite

View full text Add to dashboard Cite

A total of 33 macroscopically visible (3-11 × 1-5 mm) sarcocysts of Sarcocystis fusiformis were excised from the oesophagus of 12 freshly slaughtered water buffalos in Giza, Egypt. Genomic DNA was extracted from the sarcocysts, and all isolates were characterised at the mitochondrial cytochrome c oxidase subunit I (cox1) gene through PCR amplification and direct sequencing, whereas a few selected isolates were characterised at the 18S and 28S ribosomal (r) RNA genes and the internal transcribed spacer 1 (ITS1) region of the nuclear rDNA unit following cloning. Among the 33 cox1 sequences (1,038-bp long), there was a total of 13 haplotypes, differing from each other by one to seven substitutions and sharing an identity of 99.3-99.9 %. In comparison, the sequence identity was 98.8-99.0 % among eight complete 18S rRNA gene sequences (1,873-1,879-bp long), 98.1-100 % among 28 complete ITS1 sequences (853-864-bp long) and 97.4-99.6 % among five partial 28S rRNA gene sequences (1,607-1,622 bp). At the three nuclear loci, the intraspecific (and intra-isolate) sequence variation was due to both substitutions and indels, which necessitated cloning of the PCR products before sequencing. Some additional clones of the 18S and 28S rRNA genes were highly divergent from the more typical clones, but the true nature of these aberrant clones could not be determined. Sequence comparisons and phylogenetic analyses based on either 18S rRNA gene or cox1 nucleotide sequences, placed S. fusiformis closest to Sarcocystis cafferi from the African buffalo, but only the analyses based on cox1 data separated the two taxa clearly from each other and showed that they were separate species (monophyletic clusters and 93 % sequence identity at cox1 versus interleaved sequences and 98.7-99.1 % sequence identity at the 18S rRNA gene). Two cats experimentally infected with sarcocysts of S. fusiformis started shedding small numbers of sporocysts 8-10 days post-infection (dpi) and were euthanized 15 dpi. Sporocysts isolated from the intestinal mucosa of both cats were identified molecularly as belonging to S. fusiformis through PCR amplification and sequencing of the partial cox1. The two sporocyst-derived cox1 sequences were identical with the most common sarcocyst-derived cox1 haplotype.

show abstract

Morphological and molecular characterization and phylogenetic placement of Sarcocystis capreolicanis and Sarcocystis silva n. sp. from roe deer (Capreolus capreolus) in Norway

Cited by 40 publications

References 24 publications

Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas

Molecular identification of Sarcocystis aucheniae as the macrocyst-forming parasite of llamas

Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and cox1 sequence analysis

Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt

Contact Info

Product

Resources

About