Ultrastructure of telocytes, a new type of interstitial cells in the myocardium of the Chinese giant salamander (Andrias davidianus)

Ge, Tingting; Ye, Yaqiong; Zhang, Hui

doi:10.4081/ejh.2019.3021

Cited by 6 publications

(7 citation statements)

References 27 publications

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“…TCs are identified in a wide variety of organs from diverse species including mammals 8 – 11 , avian 12 , 13 , reptiles 14 , Amphibians 15 and aquatic species 16 , 17 and parasitic worm 18 . They are located in the trachea and lungs 19 , heart 20 and the blood vessels 21 , kidney, ureter, urinary bladder 22 , tongue 23 , oesophagus, stomach and small and large intestines 24 , liver , pancreas 14 , testis 25 , prostate 26 , efferent ductules 27 ovaries, oviducts, uterus, vagina, mammary glands and placenta 26 , spleen 8 , 28 .…”

Section: Introductionmentioning

confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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the current study investigated telocytes (tcs) in the intestinal bulb of Grass carp using light microscopy (LM), transmission electron microscopy (teM), scanning electron microscopy, and immunohistochemistry (iHc). By LM, tcs were distinguished by the typical morphological features that had a cell body and telopodes using He, toluidine blue, methylene blue, Marsland silver stain, Grimelius's silver nitrate, Giemsa, PAS, combined AB pH2,5/PAS, Crossmon's and Mallory triple trichrome, Van Gieson stains, Verhoeff's stain, Sudan black, osmic acid, performic acid with methylene blue and bromophenol blue. TCs were identified under the epithelium as an individual cell or formed a TCs sheath. They detected in the lamina propria, between muscle fibers, around the myenteric plexus and fibrous tissue. TCs acquired immunological features of endocrine cells that exhibited high affinity for silver stain, performic acid with methylene blue, Marsland stain, and immunohistochemical staining using chromogranin A. Sub epithelial tcs were closely related to the endocrine cells. tcs and their secretory activities were recognized using acridine orange. TCs were identified by IHC using CD34, CD117, S100-protein, desmin. TCs formed a3D network that established contact with macrophage, mast cells, dendritic cells, lymphocytes, smooth muscle fibers, fibroblast, Schwann cells and nerve fibers. In conclusion, the localization of TCs in relation to different types of immune cells indicated their potential role in the maintenance of intestinal immunity. Interstitial cells have critical roles in the maintenance of the appropriate 3D scaffold and functional requirements of the organs. Telocytes (TCs) are the cell population that forms a network through a labyrinthine system formed by telopodes. They are long and slender prolongations reached up to hundreds of microns. Telopodes are identified by their segments; the thin segment, podomer and the thick segment, the podoms. TCs established contact to other resident cells or to wandering cells via their telopodes or via the cell body. Telopodes network provide-long-distance cell-cell signaling (intercellular communication) 1. Paracrine signaling is critical for TCS function. TCs produce several secretory molecules and factors contributing to the functional significance.Gene analysis 2 and proteomics analysis 3 are investigated for TCs. The functional contributions of TCS are supposed to be related to angiogenesis 4 , and development, maintenance of homeostatic balance 4 , immunosurveillance 5 , tissue regeneration and repair through providing adequate microenvironment for stem cell niche and promoting their differentiation 6,7. TCs are identified in a wide variety of organs from diverse species including mammals 8-11 , avian 12,13 , reptiles 14 , Amphibians 15 and aquatic species 16,17 and parasitic worm 18. They are located in the trachea and lungs 19 , heart 20 and the blood vessels 21 , kidney, ureter, urinary bladder 22 , tongue 23 , oesophagus, stomach and small and large

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Section: Introductionmentioning

confidence: 99%

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Abd‐Elhafeez

Abou-Elhamd

Soliman

2020

Sci Rep

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“…TEM was used to observe subcellular structures of GM-preadipocytes and transdifferentiated GM-preadipocytes as described previously ( 56 ). For cells of Preadipocyte and Gpreadipocyte groups, the medium was removed at day 8, and cells were washed three times with preheated PBS.…”

Section: Methodsmentioning

confidence: 99%

In vitro transdifferentiated signatures of goat preadipocytes into mammary epithelial cells revealed by DNA methylation and transcriptome profiling

Yan¹,

Shi²,

Xiao³

et al. 2022

Journal of Biological Chemistry

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“…TEM was conducted according to our previous study ( Ge et al., 2019 ). After taking from 2.5% glutaraldehyde/PBS, small pieces of skin tissue were washed in PBS.…”

Section: Methodsmentioning

confidence: 99%

Telocytes and their structural relationships with surrounding cell types in the skin of silky fowl by immunohistochemistrical, transmission electron microscopical and morphometric analysis

et al. 2021

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Telocytes ( TCs ), a novel type of interstitial cells, were identified in various animals. Since TCs have not observed in avian skin, hence, we carried out immunohistochemistrical and transmission electron microscopical studies in the skin of the silky fowl to investigate the TCs. TCs appear as CD34, c-Kit, and PDGFRα immunopositive. The elongated TCs with 2 long and thin telopodes ( Tps ) are located in the dermis. Generally, a TC possesses a fusiform, ovoid and polygonal cell body with 2 Tps (lengths = 5.27–21.85 μm), which are uneven in thickness including thick sections – podoms (diameters = 0.40–0.47 μm) and thin sections – podomers (diameters = 0.03–0.04 μm). TCs/Tps are observed frequently in close proximity to neighboring cell types/structures, such as adipocytes, collagen fibers, and capillaries. Under a magnified field, homocellular TCs/Tps contacts are observed through gap junctions (distances = 0.01–0.05 μm), whereas some of TCs/Tps have heterocellular close contacts by point contacts with surrounding cells, including stem cells and melanocytes. The multivisicular bodies, especially exosomes (diameters = 0.09–0.23 μm) releasing from TCs/Tps are observed in close proximity to TCs/Tps. Our results illustrated that the novel type of interstitial cells – TCs are present in the dermis of the silky fowl, and they have special structural relationships with surrounding cell types. The study provides histological evidence for TCs involvement in intercellular communication, skin regeneration, and pigmentogenesis in avian skin.

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Ultrastructure of telocytes, a new type of interstitial cells in the myocardium of the Chinese giant salamander (Andrias davidianus)

Cited by 6 publications

References 27 publications

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

Morphological and immunohistochemical phenotype of TCs in the intestinal bulb of Grass carp and their potential role in intestinal immunity

In vitro transdifferentiated signatures of goat preadipocytes into mammary epithelial cells revealed by DNA methylation and transcriptome profiling

Telocytes and their structural relationships with surrounding cell types in the skin of silky fowl by immunohistochemistrical, transmission electron microscopical and morphometric analysis

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