Ultrastructure of granulopoiesis in tunica propria of the bursa of fabricius

Quesada, J.; Agulleiro, B.

doi:10.1016/0145-305x(84)90027-2

Cited by 6 publications

(4 citation statements)

References 15 publications

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“…They have large, round vesicles similar to lipid droplets and, as suggested for other vertebrates (QUESADA et al, 1985), could have the same microenvironmental significance as adipocytes, which seem to be fundamental in haemopoietic proliferation and differentiation (ALLEN and DEXTER, 1976;SORRELL and WEISS, 1980;ALLEN, 1981).…”

Section: Stromal Cellsmentioning

confidence: 99%

Stromal Cells, Macrophages and Lymphoid Cells in the Head-kidney of Sea Bass (Dicentrarchus labrax L.). An Ultrastructural Study.

Meseguer

Esteban

Agulleiro

1991

Arch. Histol. Cytol.

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Section: Stromal Cellsmentioning

confidence: 99%

Stromal Cells, Macrophages and Lymphoid Cells in the Head-kidney of Sea Bass (Dicentrarchus labrax L.). An Ultrastructural Study.

Meseguer

Esteban

Agulleiro

1991

Arch. Histol. Cytol.

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“…In addition to its role as the primary B cell organ in avian species, the embryonic bursa is also a site of granulopoiesis, with numerous granulocytes present in the bursal mesenchyme (44). We demonstrated by immunocytochemistry and flow cytometry that bursal granulocytes are CXCR4-positive.…”

Section: Cxcr4 Is Involved In Bursal Granulopoiesismentioning

confidence: 76%

In and Out of the Bursa—The Role of CXCR4 in Chicken B Cell Development

et al. 2020

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In contrast to mammals, early B cell differentiation and diversification of the antibody repertoire in chickens do not take place in the bone marrow but in a specialized gut associated lymphoid tissue (GALT), the bursa of Fabricius. During embryonic development, B cell precursors migrate to the bursa anlage, where they proliferate and diversify their B cell receptor repertoire. Around hatch these diversified B cells start to emigrate from the bursa of Fabricius and populate peripheral lymphoid organs, but very little is known how the migratory processes are regulated. As CXCL12 (syn. SDF-1) and CXCR4 were shown to be essential for the control of B cell migration during the development of lymphoid tissues in mammals, we analyzed expression and function of this chemokine/chemokine-receptor pair in the chicken bursa. We found a strong variation of mRNA abundance of CXCL12 and CXCR4 in different stages of bursa development, with high abundance of CXCL12 mRNA in the bursa anlage at embryonic day 10 (ED10). In situ hybridization demonstrated disseminated CXCL12 expression in the early bursa anlage, which condensed in the developing follicles and was mainly restricted to the follicle cortex post-hatch. Flow cytometric analysis detected CXCR4 protein already on early B cell stages, increasing during bursal development. Post-hatch, a subpopulation with the hallmarks of emigrating B cells became detectable, which had lower CXCR4 expression, suggesting that downregulation of CXCR4 is necessary to leave the CXCL12-high bursal environment. In vivo blockade of CXCR4 using AMD3100 at the time of B cell precursor immigration strongly inhibited follicle development, demonstrating that CXCL12 attracts pre-bursal B cells into the bursal anlage. Altogether, we show that CXCL12 and its receptor CXCR4 are important for both populating the bursa with B cells and emigration of mature B cells into the periphery post hatch, and that CXCR4 function in primary B cell organs is conserved between mammals and birds.

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“…The existence of myelopoiesis in the thymus of sea bass necessarily involves the arrival of circulating stem cells capable of developing in erythrocytes and granulocytes, although there is also a selective entrance of prothymocytes, and it will be the microenvironment created by the neuroendocric interactions that will favor one activity or the other, as occurs in the bursa of Fabricius of chicken embryo (Quesada et al, 1985). According to the above and in accordance with Kendall (19801, it would be convenient to refer to the thymus as a hematopoietic organ, where the cellular differentiation of the lymphoid series is favored.…”

Section: Discussionmentioning

confidence: 99%