Ultrastructure of Entamoeba gingivalis

Keller, O.; Orland, Frank J.; Baird, Grace R.

doi:10.1177/00220345670460050201

Cited by 5 publications

(2 citation statements)

References 6 publications

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“…Protozoans could have an indirect role in the development of periodontitis. Both amoebae and flagellates presented intracytoplasmic bacteria which could be alive or dead inside large food vacuoles [27,28] An in vitro study showed the capacity of two periodontal pathogens, P. gingivalis and P. intermedia, to enter, survive and replicate in Acanthamoeba castellanii, a protozoan identified in the oral cavity [29,30]. These results suggest that amoeba could be a reservoir for these bacteria and protect them from immune cells and antibiotics such as amoxicillin and participate in the re-colonization of periodontal pockets after non-surgical treatment.…”

Section: Discussionmentioning

confidence: 99%

Protozoans in subgingival biofilm: clinical and bacterial associated factors and impact of scaling and root planing treatment

Dubar

Zaffino

Rémen

et al. 2019

Journal of Oral Microbiology

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Objective: In patients with periodontitis, identification of protozoans and evaluation of some bacteria and clinical parameters associated and assessment of scaling and root planing (SRP) impact on their detection.Methods: Before and after SRP, subgingival microbiota was collected in two pathological and one healthy site from 30 periodontitis patients. One healthy site from 30 control patients was also sampled. The usual clinical periodontal parameters were recorded; microbial detection was determined by PCR hybridization system for bacteria and qPCR for protozoans.Results: In periodontitis group, Trichomonas tenax and two subtypes of Entamoeba gingivalis (ST1 and a variant ST2) were detected in respectively 33.3%, 70% and 18.3% of pathological samples, and in 6.7%, 10% and 3.3% healthy samples. In control group, ST1 alone was found in 3.3% of individuals. ST1 was associated with Gingival Index, Clinical Attachment Level (p ≤ 0.03) and with the total bacterial count (p = 0.02). T. tenax alone was associated with P. gingivalis, T. denticola and E. nodatum (p ≤ 0,02). After therapy, only T. tenax detection decreased significantly (p = 0.004) and no association between the protozoan elimination and improvement of pathological sites was found.Conclusions: Protozoans were associated with some clinical parameters and/or periodontopathogens in patients with periodontitis.

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Section: Discussionmentioning

confidence: 99%

Protozoans in subgingival biofilm: clinical and bacterial associated factors and impact of scaling and root planing treatment

Dubar

Zaffino

Rémen

et al. 2019

Journal of Oral Microbiology

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“…Since amoebae have been observed in periodontal lesions, we will test in future investigations whether 10B6 is an endosymbiont of oral amoebae, e.g. Entamoeba gingivalis (Dao et al, 1983;Gottlieb & Miller, 1971;Keller et al, 1967;Lucht et al, 1998;Yamamoto et al, 1995). Although endosymbionts have not been described for Entamoeba gingivalis, data available in the literature do not rule out their possible existence.…”

Section: Discussionmentioning

confidence: 99%

Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens

et al. 2003

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Subgingival plaque samples were obtained from 26 subjects with advanced periodontal lesions. Bacterial diversity was analysed by amplification of the 16S rRNA genes with two different primer sets, and subsequent cloning and sequencing. A total of 578 sequences was analysed after the exclusion of chimeras. The authors found 148 phylotypes with the clone library 27f/519r (number of clones n5322; coverage, C566 %) and 75 phylotypes with the clone library 515f/1525r (n5256; C584 %). Comparative sequence analysis revealed that 70 % of all of the analysed sequences showed a similarity of at least 99 % to sequences deposited in public databases. The classes Actinobacteria, Bacilli, Bacteroidetes, Clostridia, Deferribacteres, Flavobacteria, Fusobacteria, Mollicutes, Spirochaetes and all classes of the Proteobacteria were represented. Sequences that were at least 99 % identical to Porphyromonas gingivalis, Filifactor alocis and Treponema socranskii were present in at least one-third of the patients. Libraries generated with the two PCR primer pairs differed significantly in their representation of the families Porphyromonadaceae, Prevotellaceae, Fusobacteriaceae, Eubacteriaceae, Streptococcaceae and Acidaminococcaceae. A total of 14 sequences exhibited less than 97 % identity to sequences published previously and were assigned to six different families within the phyla Bacteroidetes and Firmicutes. Twelve of 20 putative pathogens were recovered, which were recently proposed to be associated with periodontitis.

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Entamoeba gingivalis and Trichomonas tenax: Protozoa parasites living in the mouth

Santos

Roldán

2023

Archives of Oral Biology

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Ultrastructure of Entamoeba gingivalis

Cited by 5 publications

References 6 publications

Protozoans in subgingival biofilm: clinical and bacterial associated factors and impact of scaling and root planing treatment

Protozoans in subgingival biofilm: clinical and bacterial associated factors and impact of scaling and root planing treatment

Molecular analysis of bacteria in periodontitis: evaluation of clone libraries, novel phylotypes and putative pathogens

Entamoeba gingivalis and Trichomonas tenax: Protozoa parasites living in the mouth

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