Ultrastructure of adult Gasterophilus intestinalis (Diptera: Gasterophilidae) and its puparium

Attia, Marwa M.; Salaeh, Nagla M.K.

doi:10.1007/s42690-019-00084-9

Cited by 17 publications

(3 citation statements)

References 15 publications

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“…Then worms were washed using 0.9% saline ( Attia and Salem, 2022 ). The collected worms were fixed in 2.5% Glutaraldehyde following to Attia and Salaeh (2020) , Abdelsalam et al (2020) , then the worms were dehydrated using ascending ethanol series, then the nematodes were dried in a CO 2 critical point drier (Autosamdri-815, Germany).…”

Section: Methodsmentioning

confidence: 99%

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

et al. 2022

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Section: Methodsmentioning

confidence: 99%

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

et al. 2022

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“…The adult nematode was fixed in 2.5% Glutaraldehyde according to Hilali et al. (2015) ; Attia and Salaeh (2020) ; then dehydrated through passing in ethanol series (50; 70; 90; 100%) and dried in CO2 critical point drier (Autosamdri-815, Germany). The adult was glued over stubs (as an anterior end and posterior end) and then; coated with 20 nm gold ( Abd ElKader et al., 2021 ) in a sputter coater (Spi-Module sputter Coater, UK).…”

Section: Methodsmentioning

confidence: 99%

Morphological and molecular characterization of Ascaridia columbae in the domestic pigeon (Columba livia domestica) and the assessment of its immunological responses

et al. 2022

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Parasitism is a divesting problem that is frequently overlooked and may result in severe prominent clinical manifestation. This study aimed to investigate the seasonal and sexual prevalence of the gastrointestinal nematode Ascaridia columbae ( A. columbae ) infection among domestic pigeons in Giza governorate, Egypt, during the period from 2020 to 2021. One hundred and sixty suspected pigeons were clinically investigated. Blood & tissue samples were collected from infected birds to estimate serum zinc concentration, malondialdehyde ( MDA ), and nitric oxide levels. As well as tumor necrosis factor-alpha ( TNF-α ), interleukin 1β ( IL1β ) activity, and histopathological examination were estimated; also, worms were collected for morphological identification using electron microscope ( SEM ) and molecularly identified using polymerase chain reaction ( PCR ), further sequenced, and submitted in GenBank with accession number MZ343369. The average ascarid (length × breadth) were 72.4 ± 3.3 µm (70.5 – 79.9 µm) × 39.9 ± 2.5 µm (37.6 – 42.3 µm). The distinguishing morphological characteristics that have been noticed in ascarid worms were creamy white, cylindrical worm with triradiate lips with wide cephalic alae extending on both the lateral sides and filariform esophagus. In males, spicules were almost equal with the presence of precloacal chitinous-rimmed sucker. The prevalence of A. columbae infection was (63.1%) with a higher incidence in females (79.2%) than males (46.1%). The highest seasonal prevalence was observed in winter (92.5%), followed by summer and spring (87.5% and 55%), respectively while, the lowest prevalence was observed in autumn (17.5%). The intensity of worms in the infected intestine varied from 5 to 120 adult worms. The histopathological examination revealed the presence of chronic diffuse moderate catarrhal enteritis with roundworms in the lumen. Infected birds showed a significant increase in nitric oxide and MDA levels while serum zinc levels were lowered in infected pigeons. Infected pigeons revealed a marked increase in IL1-β and TNFα than apparently healthy ones.

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“…After larval exposure to different concentrations of nanoparticles, all larvae were washed multiple times with buffered phosphate saline (pH 7.2) and labeled for their concentrations. The larvae were fixed for 24 h in chilled 2.5% glutaraldehyde, dehydrated in a serial upgraded ethanol degrees, dried in a CO 2 critical point drier (Autosamdri-815, Germany), and mounted on stubs with a double sticky tape before being sputter coated with 20 nm gold (Spi-Module sputter Coater, U.K.) as detailed by Attia et al [ 16 ] The larvae were imaged using a Scanning Electron Microscope (JSM 5200, Electron Probe Microanalyzer, JEOL, Japan) at the Applied Center for Entomonematodes (ACE), Agricultural Experimental Station, Faculty of Agriculture (Cairo University, Egypt).…”

Section: Methodsmentioning

confidence: 99%

Insecticidal efficacy of nanomaterials used to control mosquito, Culex quinquefasciatus Say, 1823 with special reference to their hepatotoxicity in rats

et al. 2022

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This study aimed to develop a novel methodology for controlling the mosquito larvae using different nanoparticles, with special reference to their effect on rats (a non-target mammalian model). The mosquito species of Culexquinquefasciatus was reared in the laboratory. Chitosan; silver nanoparticles and their combination as well as lavender (Lavandula officinalis) nanoemulsion with different concentrations were tested as biological insecticides against the mosquito larvae. Mammalian toxicity of the used nanoparticles were evaluated using twenty seven adult male rats, experimental rats were divided into nine equal groups (n = 3). The nanoparticles were added to the drinking water for 30 days. At the end of the study, blood and tissue samples were collected to assess the levels of the serum ALT and AST, different genes expression as Interleukin 6 (IL-6) and IL-1β activity. Histopathological and immunohistochemical studies using two markers (TNF-α and BAX expression) were also applied. The LC50 and LC90 were recorded for each tested nanoparticles, also the changes of the treated mosquito larvae cuticle were assessed using the Scanning Electron Microscopy (SEM). Conclusion: green nanoemulsion (Lavandula officinalis) was more effective than metal (silver) or even biodegradable (chitosan) nanoparticles in controlling of Culexquiquefasciatus mosquito larvae, and also it proved its safety by evaluation of the mammalian hepatotoxicity of the tested nanoparticles.

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Ultrastructure of adult Gasterophilus intestinalis (Diptera: Gasterophilidae) and its puparium

Cited by 17 publications

References 15 publications

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

Incidence of gastrointestinal parasites in pigeons with an assessment of the nematocidal activity of chitosan nanoparticles against Ascaridia columbae

Morphological and molecular characterization of Ascaridia columbae in the domestic pigeon (Columba livia domestica) and the assessment of its immunological responses

Insecticidal efficacy of nanomaterials used to control mosquito, Culex quinquefasciatus Say, 1823 with special reference to their hepatotoxicity in rats

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