Ultrastructure and Estrogen Regulation of the Lymphatic Stomata of Ovarian Bursa in Mice

Li, Meng; Zhou, Tian; Gao, Youguang; Zhang, Ning; Li, Jicheng

doi:10.1002/ar.20583

Cited by 10 publications

(9 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been suggested that the lymphatic stomata within the ovarian bursa might took part in the bursa fluid/substance circulation from the ovarian cavity to the vascular system, mainly due to its closely related structure [2], and its regulation by steroid hormones [18]. The murine bursa fluid had been described to increase 10 h after hCG administration right before ovulation [8], and the origin of fluid at this pre-ovulation period was suggested to derive partly from the plasma in the follicle walls and partly from the follicular fluid of ovulating oocytes [19].…”

Section: Discussionmentioning

confidence: 99%

Hormonal Regulation of Ovarian Bursa Fluid in Mice and Involvement of Aquaporins

Zhang

Zhao

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

In rodent species, the ovary and the end of oviduct are encapsulated by a thin membrane called ovarian bursa. The biological functions of ovarian bursa remain unexplored despite its structural arrangement in facilitating oocytes transport into oviduct. In the present study, we observed a rapid fluid accumulation and reabsorption within the ovarian bursa after ovarian stimulation (PMSG-primed hCG injection), suggesting that the ovarian bursa might play an active role in regulating local fluid homeostasis around the timing of ovulation. We hypothesized that the aquaporin proteins, which are specialized channels for water transport, might be involved in this process. By screening the expression of aquaporin family members (Aqp1-9) in the ovarian tissue and isolated ovarian bursa (0, 1, 2 and 5 h after hCG injection), we found that AQP2 and AQP5 mRNA showed dynamic changes after hCG treatment, showing upregulation at 1–2 h followed by gradually decrease at 5 h, which is closely related with the intra-bursa fluid dynamics. Further immunofluorescence examinations of AQP2 and AQP5 in the ovarian bursa revealed that AQP2 is specifically localized in the outer layer (peritoneal side) while AQP5 localized in the inner layer (ovarian side) of the bursa, such cell type specific and spatial-temporal expressions of AQP2 and 5 support our hypothesis that they might be involved in efficient water transport through ovarian bursa under ovulation related hormonal regulation. The physiological significance of aquaporin-mediated water transport in the context of ovarian bursa still awaits further clarification.

show abstract

Section: Discussionmentioning

confidence: 99%

Hormonal Regulation of Ovarian Bursa Fluid in Mice and Involvement of Aquaporins

Zhang

Zhao

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…In rodents ovarian bursa play an important role in ovulation and breeding of the animals, and the key structure executing the functions is the lymphatic stomata of ovarian bursa (Li et al, 2007). The ovarian lymphatic stomata possess the functions of active absorption and key draining, providing crucial ways for transportation of substance via in tela lymph system and serous membrane, which are of importance for the maintenance of humor homeostasis around ovary and may affect the reproductive function of ovary.…”

Section: The Distribution and Clinical Significance Of Lymphatic Stomatamentioning

confidence: 99%

Recent Advances in the Research of Lymphatic Stomata

Wang

2010

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

Lymphatic stomata are small openings of lymphatic capillaries on the free surface of the mesothelium. The peritoneal cavity, pleural cavity, and pericardial cavity are connected with lymphatic system via these small openings, which have the function of active absorption. The ultrastructure of the lymphatic stomata and their absorption from the body cavities are important clinically, such as ascites elimination, neoplasm metastasis, and inflammatory reaction. The lymphatic stomata play an important role in the physiological and pathological conditions. Our previous study indicated for the first time that nitric oxide (NO) could regulate the opening and absorption of the lymphatic stomata. It could decrease the level of free intracellular calcium [Ca 2þ ] through increasing the cyclic guanosine monophosphate (cGMP) level in the rat peritoneal mesothelial cells, thus regulating the lymphatic stomata. This process is related with the NO-cGMP- [Ca 2þ ] signal pathway. In this review, we summarize the recent advances in understanding the development and the function of the lymphatic stomata. The ultrastructure and regulations of the lymphatic stomata are also discussed in this review. Anat Rec,

show abstract

“…Sui and Li also reported that both the openings and absorptive functions of the lymphatic stomata of ovary bursa in guinea pig could be elevated by pregnancy and decreased by exogenous hormone (Sui and Li, 2003). Li et al first found that there existed estrogen receptors in the nuclei of the mesothelial cells on the visceral ovarian bursa, and suggested that both the absorption and opening area of the lymphatic stomata may be influenced by estrogen in ovarian bursa (Li et al, 2007). Li et al further screened out some Chinese herbal medicines that had efficient therapeutic implications in removing ascites.…”

Section: Discussionmentioning

confidence: 99%

Cell Signal Transduction Mechanism for Nitric Oxide Regulating Lymphatic Stomata and its Draining Capability

2008

The Anatomical Record

Self Cite

View full text Add to dashboard Cite

We have previously demonstrated that nitric oxide (NO) is involved in the regulation of the lymphatic stomata. However, the related mechanisms are still unknown. The present study was designed to test the hypothesis that NO-cyclic guanosine monophosphate (cGMP) -mediated cytosolic Ca 21 concentration ([Ca 21 ]i) signaling may contribute to the regulation of the lymphatic stomata and lymph drainage. Using trypan blue as a tracer, the effects of NO-cGMP-Ca 21 signal cascade on the lymphatic stomata and lymph absorption were examined by means of scanning electron microscopy. Then, the role of NO in cGMP and [Ca 21 ]i of rat peritoneal mesothelial cells (RPMCs) was measured by radioimmunoassay and a confocal laser scanning microscope. Our results showed that NO-donor spermine/nitric oxide complex (Sper/NO) could broaden the opening area of the lymphatic stomata and enhance lymph absorption in a dose-dependent manner. These NO-mediated changes could be blocked by 1H-[1,2,4] oxadiazolo [4,3-a] quinoxalin-1-one (ODQ), a specific inhibitor of soluble guanylyl cyclase, and mimicked by calcium channel blocker nifedipine. Furthermore, Sper/NO enhanced the cGMP level and lessened [Ca 21 ] i in RPMCs, which was completely abrogated at the presence of ODQ. Nifedipine induced an immediate and marked decrease of [Ca 21 ] i in the RPMCs, which was not attenuated by addition of Sper/NO, indicating that the Sper/NO-cGMP signaling system induced [Ca 21 ] i change was related to the L-type voltage-gated calcium channel in the RPMCs. Our results suggest that NO enlarges the opening area of the lymphatic stomata to strengthen the lymph drainage of tracer by means of NO-cGMP- [Ca 21 ]i signal transduction pathway in the RPMCs. Anat Rec, 291:216-223,

show abstract

Ultrastructure and Estrogen Regulation of the Lymphatic Stomata of Ovarian Bursa in Mice

Cited by 10 publications

References 38 publications

Hormonal Regulation of Ovarian Bursa Fluid in Mice and Involvement of Aquaporins

Hormonal Regulation of Ovarian Bursa Fluid in Mice and Involvement of Aquaporins

Recent Advances in the Research of Lymphatic Stomata

Cell Signal Transduction Mechanism for Nitric Oxide Regulating Lymphatic Stomata and its Draining Capability

Contact Info

Product

Resources

About