Hormonal Regulation of Ovarian Bursa Fluid in Mice and Involvement of Aquaporins

He, Zhong; Zhang, Ying; Zhao, Haitao; Zhang, Yunfang; Chen, Qi; Peng, Hongying; Li, Lei; Qiao, Jingqiao; Shi, Junchao; Cao, Zhen-Dong; Duan, Enkui; Jin, Yaping

doi:10.1371/journal.pone.0063823

Cited by 17 publications

(14 citation statements)

References 32 publications

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“…The expression of AQP5 was previously observed in the ovary of pigs, mice and rats (Skowronski et al 2009, Zhang et al 2013, Starowicz et al 2014. The results of several studies support the regulatory influence of steroid hormones on AQP1 mRNA and protein expression in female reproductive system (Li et al 1997, Lindsay and Murphy 2006, Jablonski et al 2003.…”

Section: Discussionsupporting

confidence: 59%

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Skowrońska¹,

Młotkowska²,

Eliszewski³

et al. 2015

Physiol Res

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Aquaporins (AQPs) are water channel proteins responsible for water homeostasis and important for proper functioning of all body systems, including reproductive structures. This study was designed to determine their localization and quantitative changes in the pig ovary during different stages of the estrous cycle and early pregnancy. The expression of AQP 1, 5 and 9 proteins was determined by immunocytochemistry and Western blot analyses. AQP1 was found in the plasma membranes of capillary endothelium, AQP5 – in the plasma membranes of granulosa cells of developing follicles and flattened follicle cells of the primordial follicles, and AQP9 – in granulosa cells of the developing follicles. In the cyclic pigs, the expression of AQP1 and 5 proteins was the highest on Days 18-20, but did not change significantly between Days 2-4, 10-12 and 14-16 of the cycle. In pregnant pigs (Days 14-16 and 30-32), the expression of AQP1 and 5 did not change and was similar to that observed during Days 10-12 and 14-16. In turn, AQP9 expression did not change between all studied periods. In conclusion, studied AQP are localized in different cells populations, the endothelial and granulosa cells, and AQP1 and 5 seem to be crucial for follicular development in pigs.

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Section: Discussionsupporting

confidence: 59%

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Skowrońska¹,

Młotkowska²,

Eliszewski³

et al. 2015

Physiol Res

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“…So far, AQP5 has been found in the ovary of mice (Zhang et al. ), rat (Starowicz et al. ), cow (Williams ) and pig (Skowronski et al.…”

Section: Discussionmentioning

confidence: 99%

“…Ovarian follicles express diverse types of AQPs depending on species (for review see Zhang et al 2012). So far, AQP5 has been found in the ovary of mice (Zhang et al 2013), rat (Starowicz et al 2014), cow (Williams 2012) and pig (Skowronski et al 2009). Notably, porcine AQP5 was immunolocalized in primary, pre-antral and antral follicles (Skowronska et al 2015), which suggests involvement of this particular AQP across all stages of follicular development.…”

Section: Discussionmentioning

confidence: 99%

Effect of Prenatal and Neonatal Anti‐Androgen Flutamide Treatment on Aquaporin 5 Expression in the Adult Porcine Ovary

Grzesiak

Knapczyk-Stwora

Mobasheri

et al. 2015

Reprod Domestic Animals

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The growth of ovarian follicles is accompanied by fluid-filled antrum formation. Water movement within the follicular wall is predominantly transcellular via membranous water channels named aquaporins (AQPs). Androgens are important regulators of mammalian folliculogenesis, and their prenatal and/or neonatal deficiency affects female fertility in adulthood. Therefore, this study was performed to determine whether gestational or neonatal exposure to the anti-androgen flutamide influences androgen-dependent AQP5 expression in pre-antral and large antral follicles of adult pigs. Flutamide was injected into pregnant gilts between days 80 and 88 of gestation and into female piglets between days 2 and 10 post-natally. The ovaries were collected from flutamide-treated and non-treated (control) sexually mature pigs. In pre-antral follicles, AQP5 mRNA and protein levels were both downregulated following maternal (p < 0.01 and p < 0.01, respectively) and neonatal (p < 0.01 and p < 0.01, respectively) flutamide exposure. Likewise, the expression of mRNA (p < 0.01 and p < 0.001, respectively) and protein (p < 0.05 and p < 0.01, respectively) for AQP5 were diminished in large antral follicles in both groups. Immunohistochemistry showed decreased intensity of AQP5 immunoreaction in pre-antral (p < 0.01) and large antral (p < 0.001) follicles following flutamide treatment. Moreover, radioimmunological analysis revealed that changes observed in AQP5 expression corresponded with diminished follicular androgens production after both maternal (p < 0.05 and p < 0.05, respectively) and neonatal (p < 0.05 and p < 0.01, respectively) flutamide administration. Therefore, AQP5 appears to be a potential regulator of follicular fluid accumulation, under androgen control, and may be a key factor in antral follicle growth.

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“…For the local injection of VACV70 into the ovarian bursa, fourweek-old female mice were anesthetized using pentobarbital sodium (50 mg/kg), and the ovaries were surgically exposed. One ovary was injected with a mixture of 0.2 μg of VACV70 and 1 μL of Lipofectamine RNAiMAX in a total volume of 10 μL with 1× PBS using a 31-gauge insulin syringe through the ovarian fat into the ovarian bursa according to previously described procedures (Zhang et al 2013). The contralateral ovary was injected with an equal volume of PBS and 1 μL of Lipofectamine RNAiMAX to serve as the control.…”

Section: Local Injection Of Vacv70 Into the Ovarian Bursamentioning

confidence: 99%

Cytosolic DNA sensor-initiated innate immune responses in mouse ovarian granulosa cells

et al. 2017

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Viral infections of the ovary may perturb ovarian functions. However, the mechanisms underlying innate immune responses in the ovary are poorly understood. The present study demonstrates that cytosolic viral DNA sensor signaling initiates the innate immune response in mouse ovarian granulosa cells and affects endocrine function. The cytosolic DNA sensors p204 and cGAS and their common signaling adaptor stimulator of interferon (IFN) genes (STING) were constitutively expressed in granulosa cells. Transfection with VACV70, a synthetic vaccinia virus (VACV) DNA analog, induced the expression of type I interferons (IFNA/B) and major inflammatory cytokines (TNFA and IL6) through IRF3 and NF-κB activation respectively. Moreover, several IFN-inducible antiviral proteins, including 2',5'-oligoadenylate synthetase, IFN-stimulating gene 15 and Mx GTPase 1, were also induced by VACV70 transfection. The innate immune responses in granulosa cells were significantly reduced by the transfection of specific small-interfering RNAs targeting , or Notably, the VACV70-triggered innate immune responses affected steroidogenesis and The data presented in this study describe the mechanism underlying ovarian immune responses to viral infection.

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Hormonal Regulation of Ovarian Bursa Fluid in Mice and Involvement of Aquaporins

Cited by 17 publications

References 32 publications

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Expression of Aquaporin 1, 5 and 9 in the Ovarian Follicles of Cycling and Early Pregnant Pigs

Effect of Prenatal and Neonatal Anti‐Androgen Flutamide Treatment on Aquaporin 5 Expression in the Adult Porcine Ovary

Cytosolic DNA sensor-initiated innate immune responses in mouse ovarian granulosa cells

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