Ultrastructure and cytochemistry ofPhaseolus leaf tissues infected with an isolate of tobacco necrosis virus inducing localized wilting

Appiano, Anna; Redolfi, P.

doi:10.1007/bf01379043

Cited by 7 publications

(8 citation statements)

References 34 publications

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“…Moreover, fibrous structures, which were frequently observed in OLV-1 and TNV-W-infected cells (35,50), were absent in BBSV-infected cells. In addition, convoluted membranes resulting from TNV-W, Leek white stripe virus, and BBSV infections differ from each other in terms of morphology (34,35). Collectively, our results revealed a distinct pattern of cytopathology derived from BBSV infection, and the low sequence identity between BBSV and other viruses may be the reason for the diverse cytopathological structures.…”

Section: Discussionmentioning

confidence: 99%

“…TNV-W infection often produced rounded dilations of up to 400 nm in the ER cisternae (35), and Olive latent virus 1 (OLV-1) infections were characterized by the presence of tonoplast-associated vesicles and extensive vesiculation of the nuclear envelope (50). Moreover, fibrous structures, which were frequently observed in OLV-1 and TNV-W-infected cells (35,50), were absent in BBSV-infected cells. In addition, convoluted membranes resulting from TNV-W, Leek white stripe virus, and BBSV infections differ from each other in terms of morphology (34,35).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies indicated that viruses from the genus Necrovirus in the family Tombusviridae often cause cytopathic effects on the tonoplast (33) or ER (34,35). Based upon this, we agroinfiltrated mCherry-fused ␥-TIP, a plant tonoplast marker (36), into N. benthamiana leaves that had been infected by BBSV.…”

Section: Bbsv Infection Reshapes the Er Structure And Generates Membrmentioning

confidence: 99%

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Morphogenesis of Endoplasmic Reticulum Membrane-Invaginated Vesicles during Beet Black Scorch Virus Infection: Role of Auxiliary Replication Protein and New Implications of Three-Dimensional Architecture

Cao

Jin

Zhang

et al. 2015

J Virol

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All well-characterized positive-strand RNA viruses[(؉)RNA viruses] induce the formation of host membrane-bound viral replication complexes (VRCs), yet the underlying mechanism and machinery for VRC formation remain elusive. We report here the biogenesis and topology of the Beet black scorch virus (BBSV) replication complex. Distinct cytopathological changes typical of endoplasmic reticulum (ER) aggregation and vesiculation were observed in BBSV-infected Nicotiana benthamiana cells. Immunogold labeling of the auxiliary replication protein p23 and double-stranded RNA (dsRNA) revealed that the ER-derived membranous spherules provide the site for BBSV replication. Further studies indicated that p23 plays a crucial role in mediating the ER rearrangement. Three-dimensional electron tomographic analysis revealed the formation of multiple ER-originated vesicle packets. Each vesicle packet enclosed a few to hundreds of independent spherules that were invaginations of the ER membranes into the lumen. Strikingly, these vesicle packets were connected to each other via tubules, a rearrangement event that is rare among other virus-induced membrane reorganizations. Fibrillar contents within the spherules were also reconstructed by electron tomography, which showed diverse structures. Our results provide the first, to our knowledge, three-dimensional ultrastructural analysis of membrane-bound VRCs of a plant (؉)RNA virus and should help to achieve a better mechanistic understanding of the organization and microenvironment of plant (؉)RNA virus replication complexes. IMPORTANCE Assembly of virus replication complexes

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Bbsv Infection Reshapes the Er Structure And Generates Membrmentioning

confidence: 99%

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Morphogenesis of Endoplasmic Reticulum Membrane-Invaginated Vesicles during Beet Black Scorch Virus Infection: Role of Auxiliary Replication Protein and New Implications of Three-Dimensional Architecture

Cao

Jin

Zhang

et al. 2015

J Virol

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“…Alpha- and beta-necroviruses usually undergo replication on membranes derived from the ER or from tonoplasts (Kassanis et al, 1970 ; Appiano and Redolfi, 1993 ; Lot et al, 1996 ). BBSV, a Betanecrovirus in the Tombusviridae family, has a single-stranded RNA genome of positive polarity (Cao et al, 2002 ; Yuan et al, 2006 ).…”

Section: D Architecture Of Plant (+) Rna Virus Replication Factoriesmentioning

confidence: 99%

Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication

et al. 2018

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Positive-sense (+) RNA viruses represent the most abundant group of viruses and are dependent on the host cell machinery to replicate. One remarkable feature that occurs after (+) RNA virus entry into cells is the remodeling of host endomembranes, leading to the formation of viral replication factories. Recently, rapid progress in three-dimensional (3D) imaging technologies, such as electron tomography (ET) and focused ion beam-scanning electron microscopy (FIB-SEM), has enabled researchers to visualize the novel membrane structures induced by viruses at high resolution. These 3D imaging technologies provide new mechanistic insights into the viral infection cycle. In this review, we summarize the latest reports on the cellular remodeling that occurs during plant virus infection; in particular, we focus on studies that provide 3D architectural information on viral replication factories. We also outline the mechanisms underlying the formation of these membranous structures and discuss possible future research directions.

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“…Virus particles were plentiful in most cells, dispersed in the cytoplasm, or forming more or less compact aggregates, some displaying a paracrystalline array, or in bleb-like evaginations of the tonoplast protruding into the vacuole ( Figure 1G). Except for the latter feature, which is more typical of tombusvirus infections (Russo et al, 1987) and had not been previously observed in OLV-1 infections (Castellano et al, 1987), the cytopathology of OLV-1/Tk did not differ substantially from that elicited by OLV-1/I or TNV serotypes in infected hosts (Castellano et al, 1987;Appiano and Redolfi, 1993).…”

Section: Double-stranded Rnas Electrophoresis Of Dsrnasmentioning

confidence: 88%

Properties of a citrus isolate of olive latent virus 1, a new necrovirus

Martelli¹,

Yılmaz

Savino³

et al. 1996

Eur J Plant Pathol

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A virus was recovered by sap transmission from plants of several citrus species exhibiting or not symptoms of chlorotic dwarf (CCD), a disease recently reported from Eastern Mediterranean Turkey. The virus was identified as an isolate of olive latent virus 1 (OLV-1), originally described as a possible sobemovirus. The citrus isolate of OLV-I (OLV-1/Tk) possesses biological, morphological, physico-chemical, and ultrastructural properties similar, if not identical to those of the OLV-1 type strain and is also serologically indistinguishable from it. In addition, OLV-1/Tk has many properties, especially physico-chemical, in common with serotypes A and D of tobacco necrosis necrovirus (TNV-A and TNV-D). However, OLV-1/Tk is only very distantly related serologically to both TNV-A and D, suggesting that it can be regarded as a distinct species in the genus Necrovirus. OLV-1/Tk could not be detected in citrus tissues by ELISA or dot-blot molecular hybridization, probably because of the extremely low virus concentration. By contrast, limited virus recovery was obtained by sap inoculation and fair detection rates were afforded by PCR. OLV-1/Tk was identified in 54 of 92 (59%) citrus plants affected by CCD and in 14 of 49 (28%) symptomless plants. These results do not support the notion that there is a canse-effect relationship between OLV-1/Tk and CCD, even though the more frequent association of this virus with diseased plants remains intriguing.

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Ultrastructure and cytochemistry ofPhaseolus leaf tissues infected with an isolate of tobacco necrosis virus inducing localized wilting

Cited by 7 publications

References 34 publications

Morphogenesis of Endoplasmic Reticulum Membrane-Invaginated Vesicles during Beet Black Scorch Virus Infection: Role of Auxiliary Replication Protein and New Implications of Three-Dimensional Architecture

Morphogenesis of Endoplasmic Reticulum Membrane-Invaginated Vesicles during Beet Black Scorch Virus Infection: Role of Auxiliary Replication Protein and New Implications of Three-Dimensional Architecture

Three-Dimensional Architecture and Biogenesis of Membrane Structures Associated with Plant Virus Replication

Properties of a citrus isolate of olive latent virus 1, a new necrovirus

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