Ultrastructural Study of Spermatogenesis in Eisenia foetida (Annelida, Oligochaeta)

Rolando, Alicia Nélida; Romanini, María Cristina; Mugnaini, María Teresa; Bozzo, Aída Andrea; Pastorino, Isabel Cecilia; Soñez, Carlos Alberto

doi:10.4067/s0717-95022007000200005

Cited by 3 publications

(3 citation statements)

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“…In all cases, the germinal cells are attached through cytoplasmic bridges to a central anucleate mass of cytoplasm rich in organelles, the cytophore. The only independent cells are the spermatozoa, which are detached from the cytophore once spermiogenesis is completed (Anderson et al, ; Rolando et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Fifty serial sections per earthworm were examined and a number of spermatogenic stages counted under a light microscope (Olympus CH20i attached to a Magus Micro Image Projection System) at 1000× magnification. The sizes of immature, premature, maturing, and fully mature spermatogenic stages in seminal vesicles (Rolando et al ) were measured using DinoCapture 2.0 Version 1.4.2.D at 400× magnification.…”

Section: Methodsmentioning

confidence: 99%

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Chronic toxicity of commercial chlorpyrifos to earthworm Pheretima peguana

Muangphra

Tharapoom

Euawong

et al. 2015

Environmental Toxicology

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A chronic toxicity study was conducted in earthworms (Pheretima peguana) exposed to soil spiked with chlorpyrifos at concentrations of 0, 0.1, 1, 10, and 100 mg/kg soil dry matter for 7, 14, and 28 days. The integrity of the coelomocyte lysosomal membrane, nervous system, and male reproductive tissue was monitored using, respectively, the neutral-red retention assay, acetylcholinesterase (AChE) enzyme assay, and histomorphology of spermatogenic cells in the seminal vesicles and cocoon production (at 28 days after 28 days' exposure). Chlorpyrifos decreased the coelomocyte neutral-red retention time (NRRT) significantly (p < 0.05) at concentrations > 0.1 mg/kg soil as early as day 7 of exposure and was dose- and time-dependent. Chlorpyrifos inhibition of AChE activity was greater at day 7 than at day14 (p < 0.05) indicating possibly nerve recovery. Chlorpyrifos induced concentration-dependent damage to spermatogenic cells and cytophores in premature stages. The number and size of premature, maturing, and fully mature spermatogenic stages were increased at low concentrations (<1 mg/kg) but a number of these maturation stages declined at higher concentrations (10 and100 mg/kg) on day 28. The most severe effects were observed in the maturing and fully mature stages at the highest chlorpyrifos concentration, and this had an adverse impact on cocoon production and cocoon viability. Collectively, the results suggest induction of widespread effects on multiple organ systems in P. peguana exposed to chlorpyrifos. Although NRRT and AChE activity were the most sensitive of the biomarkers, cocoon production and cocoon viability could still be considered as diagnostic tools for monitoring effects from low-dose long-term chlorpyrifos toxicity and for evaluating population effects. © 2015 Wiley Periodicals, Inc. Environ Toxicol 31: 1450-1459, 2016.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Chronic toxicity of commercial chlorpyrifos to earthworm Pheretima peguana

Muangphra

Tharapoom

Euawong

et al. 2015

Environmental Toxicology

View full text Add to dashboard Cite

show abstract

“…These cells are elongated (length up to 50 lm) with a thickness around 500 nm (Rolando et al, 2007) and offer the possibility to create a stitched 3-D tomographic reconstruction of an entire eukaryotic cell. Because of their elongated shape it is possible to acquire adjacent tilt series along the cell body.…”

Section: Batch Tomography Of An Elongated Whole Cellmentioning

confidence: 99%