Ultrastructural Studies on the Leucocytes and Thrombocytes in the Circulating Blood of Japanese Quail ,

Nirmalan, G.; Atwal, O. S.; Carlson, H. C.

doi:10.3382/ps.0512050

Cited by 8 publications

(14 citation statements)

References 10 publications

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“…Separate morphometric analyses on the white blood cells of the rheas reared in the states of ES and SP showed that the length and width measurements were also larger than those reported in the literature for birds in general and that some cells were of sizes within the range of measurements for birds (Lucas and Jamroz, 1961;Nirmalan et al, 1972;Campbell and Dein, 1984;Banks, 1993;Coles et al, 1998;Jain et al, 2000). Comparative morphometric analysis on these cells between rheas reared in ES and in SP showed that the leukocytes of the birds in ES had greater length and width than those in SP, except for the widths of eosinophils, which were not statistically different.…”

Section: Discussionmentioning

confidence: 95%

Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae) on two conservation farms

2017

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Today, blood tests are an indispensable tool in avian medicine. This study aimed to describe and compare hematological and morphometric data of erythrocytes, leukocytes and thrombocytes of rhea, Rhea americana. To do so, 58 rheas of both sexes on two farms, one in Cachoeiro do Itapemirim, Espírito Santo (ES), and the other in São Carlos, São Paulo (SP), were selected. Blood samples were taken and their RBC counts, PCV and Hb levels were determined and used in hematimetric index calculations. The total and differentiated leukocyte counts and the TPP and fibrinogen serum levels were also obtained. The results from the two farms analyzed were compared by means of the t test. There were differences in the parameters of the erythrocyte series between the two flocks because of a process of hypochromic macrocytic anemia observed among the rheas in ES. The values for the erythrocyte series and indexes were: RBC, 2.81 ± 0.15 x10 6 /μl; PCV, 44.20 ± 2.86%; Hb, 12.12 ± 0.74 g/dL; MCV, 15.75 ± 0.89 fL; MCH, 43.18 ± 1.82 pg; and MCHC, 27.44 ± 0.80 g/dL. The values for the leukocyte series were: WBC, 12,072 ± 4116 /μL; heterophils, 64.10 ± 9.90%; eosinophils, 2.05 ± 2.06%; monocytes, 6.40 ± 2.99%; lymphocytes, 26.93 ± 9.62%; and basophils, 0.52 ± 1.27%. These can all be suggested as references for rheas reared in Brazil. Statistical differences were observed in erythrocyte length measurements, which were higher in the rheas in SP than in those in ES. Regarding width, the heterophils, lymphocytes and monocytes of the birds in ES were statistically greater than those of the birds in SP. It was concluded that rheas may exhibit morphometric alterations to blood cells and differences in blood elements according to the type of management to which they are subjected. Hto, 44,20±2,86%; Hb, 12,12±0,74 g/dL; VCM, 15,75±0,89 fL; HCM, 43,18±1,82 pg; CHCM, 27,44±0,80, além dos valores da série branca como: leucometria total, 12.072±4.116/μL, heterofilo, 64,10±9,90%, eosinófilo, 2,05±2,06%, monócito, 6,40±2,99%, linfócito, 26,93±9,62%, basófilo, 0,52±1,27% foram sugeridos como os de referência para as emas criadas no Brasil. Diferenças estatísticas foram observadas no comprimento de eritrócitos, o qual estava maior nas emas de SP do que aquelas do ES. Em relação à largura, os heterófilos, linfócitos e monócitos das aves do ES foram estatisticamente maior do que aquelas das aves de SP. Concluiu-se que emas podem apresentar alterações morfométricas de células sanguíneas e diferenças nos elementos do sangue de acordo com o tipo de manejo a que são submetidos. KeywordsPalavras-chave: patologia clínica, testes sanguíneos, morfometria, ratitas.

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Section: Discussionmentioning

confidence: 95%

Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae) on two conservation farms

2017

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“…However, were found heterophils with length and width of 9.04 and 7.92 µm, which is within the range of reference values of 5.1 and 11.4 µm in diameter for these cells in birds (Lucas and Jamroz, 1961;Nirmalan et al, 1972;Campbell and Dein, 1984;Jain et al, 2000). This was also observed for monocytes, with some cells having a length and width far below the 12 µm diameter reported by Lucas and Jamroz (1961) for avian monocytes.…”

Section: Discussionmentioning

confidence: 95%

“…For example, the mean morphometric index (MI) calculated for all of the white blood cells in rheas is close to one. This may explain why the length and width of these cells is reported as the measured diameter (Lucas and Jamroz, 1961;Nirmalan et al, 1972;Campbell and Dein, 1984;Banks, 1993;Coles et al, 1998;Jain et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

“…In general, the heterophil nucleus is polymorphonuclear, with one to five lobulations with dense chromatin. These cells are amoeboid and have motility (Lucas and Jamroz, 1961;Nirmalan et al, 1972;Campbell and Dein, 1984;Jain et al, 2000). The staining of the nucleus is basophilic with darker regions representing condensed chromatin (Lucas and Jamroz, 1961).…”

Section: Introductionmentioning

confidence: 99%

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Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilian birds

et al. 2015

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Blood exams are an indispensable tool in bird medicine. This study aimed at describing values and aspects of rheas' hematology, Rhea americana, as well as analyzing the morphology and morphometry of all blood cells. Fifty eight adult rheas of both sexes from two farms, one in Cachoeiro de Itapemirim, Espírito Santo State and the other in São Carlos, São Paulo State, were selected. Blood samples were taken and RBC count, PCV and Hb levels measured and used in hematimetric indexes calculations. The total and differentiated leukocyte counts, as well as the TPP and fibrinogen were determined. Erythrocytes, leukocytes and thrombocytes were identified and characterized morphologically. (27,44±0,80 g/dL); os valores da série branca foram: CTL (12,072±4116/μL), heterófilos (64,10±9,90%), eosinófilos (2,05±2,06%), monócitos (6,40±2,99%), linfócitos (26,93±9,62%), basófilos (0,52±1,27%). Pode-se concluir que, na média, células sanguíneas de emas são maiores que as de outras aves, porém, não é possível diferenciar estas células em esfregaços somente pelo seu tamanho. Além disso, leucócitos de emas possuem diferentes componentes e coloração como acontece em outras espécies de aves, no entanto, não há nenhum componente ou aspectos tintoriais exclusivo para a espécie.Palavras-chave: patologia clínica, hemograma, proteínas, ratitas, Rhea americana.

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“…This was calculated for all white cells of emus and is close to 1 (Table 2). This may explain why length and width of these cells are used as observed diameter of these cells (Nirmalan et al 1972;Lucas and Jamroz 1961;Campbell and Dein 1984;Banks 1993;Coles et al 1998;Jain et al 2000).…”

Section: Discussionmentioning

confidence: 94%

Clinical aspects, morphometry, and morphology of blood cells of the emu (Dromaius novaehollandiae) reared in captivity

et al. 2015

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The emu, or Australian ostrich (Dromaius novaehollandiae), is a native to the Australian plains. Understanding the hematology and clinical aspects of emus is necessary so as to outline sanitary measures and guarantee production and breeding management in captivity. This study evaluates the clinical aspects, the morphometry, and the morphology of emu blood cells reared in captivity, in the city of Campos dos Goytacazes, state of Rio de Janeiro, Brazil. Fifteen animals of either sex reared in the scientific nursery of Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF) were clinically examined before egg hatching. This group included three males, which also started the egg incubation period, that were analyzed during two periods (during incubation and off incubation). Blood samples were collected, and smears were prepared and stained for morphometric and morphological analyses. The results show that the peculiar behavior of emus during incubation triggers important changes in clinical status, like weight loss, reduction in rectal temperature, and in heart and respiratory rates of animals. Blood cell morphometry revealed that heterophil had the lowest morphometric index and were the most closely spherical cells in the emus examined. Erythrocytes presented the highest morphometric index, which lends this cell type its ovoid shape.

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Ultrastructural Studies on the Leucocytes and Thrombocytes in the Circulating Blood of Japanese Quail ,

Abstract: The ultrastructure of the leucocytes and thrombocytes in the peripheral blood of Japanese quail was studied. The fine structure of these cells were fundamentally identical with those of other Aves. A brief description of the morphology of the cells examined has been presented.

Cited by 8 publications

References 10 publications

Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae) on two conservation farms

Hematological and morphometric differences of blood cells from rheas, Rhea americana (Struthioniformes: Rheidae) on two conservation farms

Hematological, morphological and morphometric characteristics of blood cells from rhea, Rhea Americana (Struthioniformes: Rheidae): a standard for Brazilian birds

Clinical aspects, morphometry, and morphology of blood cells of the emu (Dromaius novaehollandiae) reared in captivity

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