Ultrastructural, morphometric, and hormonal analysis of the effets of testosterone treatment on leydig cells and other interstitial cells in young adult rats

Ichihara, Ichiro; Kawamura, Hideto; Nakano, Takashi; Pelliniemi, Lauri J.

doi:10.1016/s0940-9602(01)80196-5

Cited by 12 publications

(8 citation statements)

References 52 publications

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“…The increased number of Leydig cells in the cadmium group of this study may be due to the differentiation of immature precursor cells that have the capacity to repopulate the testis, after destruction of the mature cell population due to cadmium treatment [39,40]. Also, Blanco et al [3] affirms that the cadmium withdrawal led to a hyperplasia of this cell population, which could be an answer to offset the morphologic damage incurred.…”

Section: Discussionmentioning

confidence: 66%

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Predes

Monteiro

Matta

et al. 2010

Biol Trace Elem Res

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The aim of this study is to investigate the association of a single low dose of Cd and daily doses of Ginkgo biloba extract (GbE) on the testis and accessory glands of rats. The animals were treated with a single dose of 3 µmol/kg body weight of cadmium chloride (CdCl₂) and/or 100 mg/kg body weight of GbE. The plasma testosterone levels; corporal, testicular, and accessory glands weight; gonadosomatic index, volumetric proportion; and absolute volume of testicular components did not change after the treatments. CdCl₂ caused significant reduction in Leydig cells volume and altered Leydig cell morphology, as well as vacuolated Sertoli cells cytoplasm, irregular chromatin condensation of late spermatids, and modified acrosome formation. However, animals that received GbE did not show these alterations. The reversal of Cd-induced alterations by the extract is a strong indication that G. biloba is helpful in diminishing the effect of Cd toxicity.

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Section: Discussionmentioning

confidence: 66%

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Predes

Monteiro

Matta

et al. 2010

Biol Trace Elem Res

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“…Both LH and testosterone are responsible for normal spermatogenesis in male rats [24]. Therefore, depletion of LH receptors and decreases in peripheral LH by exogenous testosterone administration result in the reduction of testosterone secretion [25].…”

Section: Pathological Changes Of Testes Ultrastructurementioning

confidence: 99%

Transmission Electron Microscopy for the Quantitative Analysis of Testis Ultra Structure

Shokri¹,

Hemadi²,

Aitken³

2012

The Transmission Electron Microscope

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“…Furthermore, steroidogenesis has been reported to take place within the Leydig cells of the testis, and the production of testosterone is influenced by the activity of LH on the Leydig cells. Thus LH depletion will result in reduced testosterone production (Ichihara, Kawamura, Nakano, & Pelliniemi, ). However, the highest testosterone concentration was found in the HAART‐treated animals which appears discordant with the degenerative changes such as decreased Leydig cells, reduction in Leydig cell nuclei diameters and volumes seen in the interstitium of this group at ultrastructural level.…”

Section: Discussionmentioning

confidence: 99%

Adjuvant potential of virgin coconut oil extract on antiretroviral therapy-induced testicular toxicity: An ultrastructural study

et al. 2017

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The effects of Virgin coconut oil as an adjuvant to highly active antiretroviral therapy (HAART) were investigated on the testicular ultrastructure and biochemical markers in rats. Twenty male Sprague-Dawley rats, weighing 153-169 g were divided into four groups and treated as follows: control A (distilled water), B (HAART), C (HAART+Virgin coconut oil 10 ml/kg) and D (Virgin coconut oil [VCO] 10 ml/kg). Testicular segments were evaluated using transmission electron microscopy. Serum was assayed for testosterone, luteinising hormone, follicle stimulating hormone and testicular tissue for malondialdehyde and glutathione. Ultrastructure of basement membrane (Bm), mitochondria and spermatocytes was normal in the control group. HAART-treated group showed significant increase (p < .01) in Bm thickness with significant decrease in Leydig cell nuclear diameter (p < .05) and volume (p < .01) when compared with control group. Mitochondrial cristae appear collapsed, and Sertoli cells showed cytoplasmic vacuolations. HAART+VCO group showed improved ultrastructural details in Bm, and Sertoli cell and Leydig cells show abundant lipid droplets. Virgin coconut oil-treated group showed thinning of Bm with otherwise normal ultrastructural features of organelles. HAART-treated group showed significant increase (p < .01) in testosterone levels. There was no significant effect on malondialdehyde and glutathione levels. Virgin coconut oil improved testicular morphology and reversed HAART-induced ultrastructural alterations. Further studies on putative mechanism are required.

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Ultrastructural, morphometric, and hormonal analysis of the effets of testosterone treatment on leydig cells and other interstitial cells in young adult rats

Cited by 12 publications

References 52 publications

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Testicular Histomorphometry and Ultrastructure of Rats Treated with Cadmium and Ginkgo biloba

Transmission Electron Microscopy for the Quantitative Analysis of Testis Ultra Structure

Adjuvant potential of virgin coconut oil extract on antiretroviral therapy-induced testicular toxicity: An ultrastructural study

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