Transmission Electron Microscopy for the Quantitative Analysis of Testis Ultra Structure

Shokri, Saeed; Hemadi, Masoud; Aitken, Robert J.

doi:10.5772/36743

Cited by 10 publications

(8 citation statements)

References 42 publications

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“…Ultrastructurally, the changes of the thickness of basal lamina observed in the present research could be explained by the study of Shokri et al [50] who found that certain stimulants might encourage the myoid cells to yield more collagen and extracellular matrix, which cause the thickening of the basal lamina. In the current work, numerous intercellular areas were noticed in-between the germinal cells lining the seminiferous tubules.…”

Section: Discussionsupporting

confidence: 59%

Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

2015

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Section: Discussionsupporting

confidence: 59%

Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

2015

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“…In another study by Shokri, Hemadi, and John Aitken (), empty vacuolar spaces that contain lipid droplets were observed in the Sertoli cells in experimental group (exposure to nanomaterial). In comparison with control group, irregular and thickened basal lamina and cytoplasmic vacuolation in the Leydig cells also were seen in experimental group.…”

Section: Discussionmentioning

confidence: 91%

Morphological and ultrastructural studies of human spermatogonial stem cells from patients with maturation arrest

et al. 2016

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Destruction of spermatogonial stem cells (SSCs) along the chemotherapy and radiotherapy is one of the side effects of cancer treatments that lead to infertility. In vitro propagation of hSSCs is necessary to obtain an adequate number of cells for successful transplantation. In this study, hSSCs were isolated from testis biopsies of the patients with maturation arrest and proliferated in DMEM in the presence of LIF and bFGF for 5 weeks. The various types of human spermatogonia were identified in culture system and compared with testis tissue using morphological criteria at the ultrastructural level. The results showed that although many various types of spermatogonia were identified, but no remarkable difference was observed between spermatogonial cells in culture system and testis tissue. Electron and light microscopic studies of hSSC colonies did not show differentiated SSCs in the culture system. The results also showed that probably the suitable time for transplanting of SSCs in recipient testis is 2-3 weeks after culture. Because apoptosis which may affect the development of germ cells has not started in colony cells at this time and the population of apoptotic cells are low.

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“…In turn, these descriptions of the ultrastructure of spermatogonia in these mammalian species appear to be comparable with the observations of our study and could be generalized for most mammalian species. Russell et al (1990) and Shokri, Hemadi, and Aitken (2012) noted that the transformation from primary spermatocyte to secondary spermatocyte and to spermatid is rarely seen in ultrastructural assessments of the testes. This is because of the rapid rate at which this transformation occurs.…”

Section: Discussionmentioning

confidence: 99%

Morphology and ultrastructure of the testes and spermatozoa of the South African leopard (Panthera pardus)

Neves

Wagenaar

2018

Acta Zoologica

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The survival of South African leopard (Panthera pardus) populations is at risk due to the decrease in natural habitat caused by increasing human populations, development projects and industry. The use of ex situ conservation methods such as assisted reproductive techniques (ARTs) in captive breeding programmes can be used to conserve and protect this species. However, a good understanding of the reproductive system of the species is imperative in the development of successful ARTs. This study serves to provide information on the morphology and ultrastructure of the spermatozoa and testes of P. pardus that can be used to help in the development, testing and optimization of reproductive techniques. The objectives of this study were (i) to assess the morphology and ultrastructure of the spermatozoa by means of fluorescence and electron microscopy respectively, and (ii) to examine the morphology and ultrastructure of the testicular tissue as well as of the stages of spermatogenesis using light and electron microscopy. In this study, it was noted that the morphology and ultrastructure of the spermatozoa are similar to those reported in other feline and mammalian species and that a high amount of morphologically abnormal ejaculated sperm was found to be a common occurrence in all samples obtained.

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Transmission Electron Microscopy for the Quantitative Analysis of Testis Ultra Structure

Cited by 10 publications

References 42 publications

Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

Morphological and ultrastructural studies of human spermatogonial stem cells from patients with maturation arrest

Morphology and ultrastructure of the testes and spermatozoa of the South African leopard (Panthera pardus)

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