Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

Hussein, Adel M.; Mustafa, Hesham N.; Badawoud, Mohammed H.

doi:10.5603/fm.2015.0049

Cited by 3 publications

(2 citation statements)

References 52 publications

(58 reference statements)

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“…Due to its high toxicity and cumulative effect, Cd easily leads to multi-organ injury, especially to the genital system [4-6]. Compared with other organs, the testes, which are the male gonads, are more vulnerable to toxicants because of their active cell division and metabolism [7-11]. A recent report used the measurement of semen quality as a marker of environmental pollution due to cadmium [12].…”

Section: Introductionmentioning

confidence: 99%

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Wang

Chang

Peng

et al. 2017

Cell Physiol Biochem

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Background: Cadmium (Cd), a common environmental heavy metal and endocrine disruptor, is known to exert toxic effects on the testes. However, the mechanisms accounting for its toxicity in mature spermatozoa remain unclear. Methods: Adult male C57BL/6 mice were orally administered with CdCl₂ for 5 weeks at 3 mg·kg^-1·day^-1. Additionally, mouse spermatozoa were incubated in vitro with different doses of CdCl₂ (0, 10, 50, 250 µM). Several sperm functions including the sperm motility, viability and acrosome reaction (AR) ratio were then examined. Furthermore, the current and expression levels of both the sperm-specific Ca²⁺ channel (CatSper) and the sperm-specific K⁺ channel (KSper) were evaluated by patch-clamping and western blotting, respectively. Results: Our data showed that the motility, viability and AR of sperm exposed to cadmium significantly decreased in vivo and in vitro. Interestingly, these changes were correlated with changes in CatSper but not KSper. Conclusion: The findings indicate sperm dysfunction during both chronic and acute cadmium exposure as well as a specific role for CatSper in the reproductive toxicity of cadmium.

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Section: Introductionmentioning

confidence: 99%

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Wang

Chang

Peng

et al. 2017

Cell Physiol Biochem

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“…The mean diameters of the tubules were obtained by estimating across the minor and major axes. The height of germ cells epithelium was estimated as the space from the tunica propria to the edge of the seminiferous lumen and two opposed readings were analyzed using their mean value [21].…”

Section: Histological Analysismentioning

confidence: 99%

Formalin Versus Bouin Solution for Rat Testicular Tissue Fixation: A Histochemical and Immunohistochemical Evaluation

Waleed Aziz,

Ghassan Saeed,

Turath Tawfeeq

2023

IJMTFM

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Background: An accurate histopathological assessment and reporting of testicular biopsies require an appropriate tissue fixative. We assessed the histological, histochemical, and immunohistochemical quality of testicular biopsies, comparing 10% formalin versus Bouin solution as tissue fixatives. Methods: This experimental study utilized 20 adult male albino rats equally divided into five cages for 30 days. By the end of the experiment, all animals were anesthetized, and both testes were removed and weighted; one testicle was fixed in 10% formalin and the other testicle in Bouin solution, offering 40 specimens and then subjected to histological, morphometric, histochemical, and immunohistochemical assessments. Results: Formalin revealed high-quality cytological details and better nuclear chromatin detail (P=0.03). At the architectural level, the Bouin solution showed better quality details with less cytoplasmic shrinkage of seminiferous tubule germ cells (P=0.001). Bouin’s fixed tissues were more suitable for staining by trichrome methods but unsuitable when subsequent immunohistochemistry was requested. The diagnostic concordance between the Bouin solution versus formalin-fixed biopsies was 91.7%. Conclusion: This study supports that the morphology of testicular tissue fixed with Bouin solution was nearly comparable to those fixed with 10% neutral buffered formalin. However, the Bouin solution cannot substitute formalin when subsequent immunohistochemistry is considered.

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The role of curcumin in streptozotocin-induced hepatic damage and the trans-differentiation of hepatic stellate cells

Mustafa

2016

Tissue and Cell

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Ameliorative potentials of a combination of fenugreek and alpha-tocopherol on cadmium induced testicular toxicity: an ultrastructural study

Abstract: (Folia Morphol 2015; 74, 3: 325-334)

Cited by 3 publications

References 52 publications

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Exposure to Cadmium Impairs Sperm Functions by Reducing CatSper in Mice

Formalin Versus Bouin Solution for Rat Testicular Tissue Fixation: A Histochemical and Immunohistochemical Evaluation

The role of curcumin in streptozotocin-induced hepatic damage and the trans-differentiation of hepatic stellate cells

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