Ultrastructural markers of quality are impaired in human metaphase II aged oocytes: a comparison between reproductive and in vitro aging

Bianchi, Serena; Macchiarelli, Guido; Micara, Giulietta; Linari, Antonella; Boninsegna, C; Aragona, Cesare; Rossi, Gianna; Cecconi, Sandra; Nottola, Stefania Annarita

doi:10.1007/s10815-015-0552-9

Cited by 67 publications

(63 citation statements)

References 84 publications

(150 reference statements)

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“…As previously stated, if fertilization does not occur within the appropriate period, unfertilized oocytes remain in the oviduct or culture medium and undergo oocyte aging, which impairs oocyte competence . In our facility, the percentage of blastocysts gradually worsened in a time‐dependent manner over a 5‐hour period before ICSI (data not shown).…”

Section: Discussionsupporting

confidence: 66%

“…Therefore, optimization of oocyte aging should improve laboratory and clinical outcomes in assisted reproductive technology. However, in addition to factors that may adversely affect oocyte aging, extended in vitro culture may impair oocyte competence . For example, Wakayama et al reported negative effects of temperature change on mouse oocyte aging, while Goud et al indicated that the production of reactive oxygen species significantly enhanced oocyte aging.…”

Section: Introductionmentioning

confidence: 99%

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The timing of cumulus cell removal for intracytoplasmic sperm injection influences the capability of embryonic development

Mizuno

Ishikawa

Matsumoto

et al. 2018

Reprod Medicine & Biology

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PurposeTo determine whether the presence of intact cumulus cells during the preincubation period for ICSI should be considered as a critical factor in fertilization and embryonic development.MethodsThe cohort of this prospective randomized study was limited to infertile women younger than 39 years of age who underwent controlled ovarian stimulation for ICSI between October 2013 and May 2015 and whose embryos were to be incubated until day 5. Women with estradiol levels of <2000 pmol/L on the day of HCG injection were excluded. Cumulus cells were removed immediately after OPU in Group A and at 120 minutes after OPU in Group B. ICSI was performed with all mature oocytes, and fertilized oocytes were cultured to the blastocyst stage. Maturation, fertilization, blastocyst, good quality blastocyst, pregnancy, live birth, and miscarriage rates were compared.ResultsThere were no significant differences in maturation, fertilization, blastocyst, pregnancy, live birth, or miscarriage rates between Groups A and B. However, the percentage of good quality blastocysts was significantly higher in Group B than Group A (52.0% vs 33.1%).ConclusionsIntact cumulus cells should be maintained during the preincubation period, as they are important to embryonic development after fertilization.

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Section: Discussionsupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

The timing of cumulus cell removal for intracytoplasmic sperm injection influences the capability of embryonic development

Mizuno

Ishikawa

Matsumoto

et al. 2018

Reprod Medicine & Biology

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“…As a woman gets older, an evident decrease in fecundity occurs in parallel, due to both a reduction of the ovarian follicle numbers and an impairment of oocyte quality as well. In particular, by age 35 a woman's chance of conceiving per month is decreased by half and, at the age of 45, the natural fertility rate per month drops to 1% [14]. Thus, postponing motherhood for social, educational or financial reasons may significantly decrease the chance for a woman to become pregnant and/or increase the risk of obstetric complications [15].…”

Section: Age-related Infertilitymentioning

confidence: 99%

Technologies for the Production of Fertilizable Mammalian Oocytes

et al. 2019

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Women affected by ovarian pathologies or with cancer can usually preserve fertility by egg/embryo freezing. When oocyte retrieval is not feasible, the only option available is ovarian tissue cryopreservation and transplantation. The culture of follicles isolated from fresh or cryopreserved ovaries is considered still experimental, although this procedure is considered safer, because the risk of unintentional spreading of cancer cells eventually present in cryopreserved tissue is avoided. Animal and human small follicles can be cultured in vitro, but standardized protocols able to produce in vitro grown oocytes with the same developmental capacity of in vivo grown oocytes are not available yet. In fact, the different sizes of follicles and oocytes, the hormonal differences existing between mono- (e.g., human, goat, cow, and sheep) and poly-ovulatory (rodents and pig) species, and the incomplete identification of the mechanisms regulating the oocyte–follicle and follicle–ovary interrelationships affect the outcome of in vitro culture. From all these attempts, however, new ideas arise, and the goal of assuring the preservation of female reproductive potential appears a more realistic possibility. This review surveys and discusses advances and challenges of these technologies that, starting from a simple attempt, are now approaching the biosynthesis of a functional engineered ovary.

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“…In fact, up to one in six couples in Western countries is affected by infertility [7]. The most important risk factors in the development of female subfertility include: the age of the woman [6], ovulatory disorders, chromosomal abnormalities, and defective male fertility [8][9][10]. A widespread variety of environmental pollutants, such as several classes of pesticides, heavy metals, and air particulate matter plays a key role in the pathogenesis of female infertility [8,11,12].…”

Section: Introductionmentioning

confidence: 99%

Association between Female Reproductive Health and Mancozeb: Systematic Review of Experimental Models

Bianchi

Nottola

Torge

et al. 2020

IJERPH

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Mancozeb is a widely used fungicide approved for use in agriculture in many countries with long persistence in the environment and consequent bioaccumulation in tissues and biological fluids. Despite the large amount of studies published in recent years, the relationship between mancozeb exposure and female reproductive health is not fully elucidated. In order to summarize current evidence on mancozeb exposure and female reproductive disease, we performed a systematic review of literature. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to make this review. An adapted version of the National Toxicology Program's Office of Health and Assessment and Translation (OHAT) framework was used to evaluate the risk of bias. Electronic search on two databases (PubMed and Scopus) was used to find experimental studies (in vitro and in vivo) on mancozeb exposure. The database search identified 250 scientific articles, 20 of which met our inclusion criteria. Selected data were then reviewed and summarized in tables. Overall, mancozeb represents a hazard for female reproductive health, with different mechanisms of action. Undoubtedly more experimental and epidemiological studies are required to definitively validate mancozeb as reproductive toxicant.

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Ultrastructural markers of quality are impaired in human metaphase II aged oocytes: a comparison between reproductive and in vitro aging

Cited by 67 publications

References 84 publications

The timing of cumulus cell removal for intracytoplasmic sperm injection influences the capability of embryonic development

The timing of cumulus cell removal for intracytoplasmic sperm injection influences the capability of embryonic development

Technologies for the Production of Fertilizable Mammalian Oocytes

Association between Female Reproductive Health and Mancozeb: Systematic Review of Experimental Models

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