Ultrastructural investigation of acidocalcisomes and ATPase activity in yeast
            <i>Candida guilliermondii</i>
            NP‐4 as ‘complementary’ stress‐targets

Hovnanyan, Karlen; Marutyan, S.; Hovnanyan, Margarita; Navasardyan, Liparit; Trchоunian, Armen

doi:10.1111/lam.13348

Cited by 6 publications

(3 citation statements)

References 23 publications

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“…Under manganese stress, an increase in the content and chain length of acid-soluble polyP was observed in the cells of Saccharomyces cerevisiae [24] and Cryptococcus humicola [25]. In the cytoplasm of Candida guilliermondii, the size of electron-dense volutin granules containing polyP increased under the influence of X-irradiation [26].…”

Section: Introductionmentioning

confidence: 99%

Stress Resistance of Saccharomyces cerevisiae Strains Overexpressing Yeast Polyphosphatases

et al. 2022

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Inorganic polyphosphate (polyP) is an important factor in the stress resistance of microorganisms. The polyphosphate-overexpressing strains of yeast S. cerevisiae were used as a model for studying the inter-relationship between stress resistance and polyP level. We compared the polyP level and resistance to the oxidative, manganese, cadmium, and alkaline stresses in parent stain CRN and in strains overexpressing the four yeast polyphosphatases: Ppx1, Ppn1, Ppn2, and Ddp1. Strains overexpressing Ppx1, Ppn1, and Ppn2 have lower polyP content and the strain overexpressing Ddp1 has the same polyP content as the parent strain. The strains overexpressing Ppn1 and Ddp1 show higher resistance to peroxide and manganese. The strain overexpressing Ppx1 showed a decrease in peroxide resistance. The strain overexpressing Ppn2 was more resistant to alkaline and peroxide stresses. A similar increase in resistance to the manganese and peroxide stresses of strains overexpressing Ppn1 and Ddp1, which differ in polyP content, led to the conclusion that there is no direct relationship between polyP content and variations in this resistance. Thus, we speculate about the potential role of inositol pyrophosphates as signaling molecules in stress response.

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Section: Introductionmentioning

confidence: 99%

Stress Resistance of Saccharomyces cerevisiae Strains Overexpressing Yeast Polyphosphatases

et al. 2022

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“…Biology 2021, 10, 487 2 of 13 In yeast, polyPs are involved in the control of the cell cycle [12], stress response [13][14][15], and virulence [16]. Yeast genomes do not contain orthologs of bacterial polyphosphate kinases, and polyP synthesis is performed by the Vtc4 protein [17].…”

Section: Introductionmentioning

confidence: 99%

VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells

Tomashevsky

Kulakovskaya

Trilisenko

et al. 2021

Biology

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Inorganic polyphosphate (polyP) is an important factor of alkaline, heavy metal, and oxidative stress resistance in microbial cells. In yeast, polyP is synthesized by Vtc4, a subunit of the vacuole transporter chaperone complex. Here, we report reduced but reliably detectable amounts of acid-soluble and acid-insoluble polyPs in the Δvtc4 strain of Saccharomyces cerevisiae, reaching 10% and 20% of the respective levels of the wild-type strain. The Δvtc4 strain has decreased resistance to alkaline stress but, unexpectedly, increased resistance to oxidation and heavy metal excess. We suggest that increased resistance is achieved through elevated expression of DDR2, which is implicated in stress response, and reduced expression of PHO84 encoding a phosphate and divalent metal transporter. The decreased Mg2+-dependent phosphate accumulation in Δvtc4 cells is consistent with reduced expression of PHO84. We discuss a possible role that polyP level plays in cellular signaling of stress response mobilization in yeast.

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“…In yeast, polyPs are involved in the control of the cell cycle [12] and stress response [13][14][15]. The Vacuole Transporter Chaperone (VTC) complex, which comprises Vtc4 subunit catalyzing polyP biosynthesis, plays an important role in vacuolar membrane fusion and has physical relations with vacuolar H + -ATPase (V-ATPase), influencing vacuolar H + uptake [16,17].…”

Section: Introductionmentioning

confidence: 99%

Role of VTC4 in Stress Response and Regulation of Inorganic Polyphosphate Levels in Yeast

Tomashevsky¹,

Kulakovskaya²,

Trilisenko³

et al. 2020

Preprint

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Inorganic polyphosphate (polyP) is an important factor of stress tolerance in microbial cells. In yeast, the major enzyme of polyP biosynthesis is Vtc4, a subunit of the vacuole transporter chaperone (VTC) complex. In this study, we demonstrated that Vtc4 knockout in Saccharomyces cerevisiae not only decreased polyP content but also caused shifts in the composition of the intracellular polyP pool and changed the stress tolerance profile. In the mutant S. cerevisiae, the level of short-chain acid-soluble polyPs was decreased nearly 10-fold, whereas that of longer acid-insoluble polyPs was decreased only 2-fold, suggesting the existence of other enzymes compensating the production of long-chain polyPs. The Δvtc4 mutant showed inhibition of Mg2+-dependent phosphate uptake and decreased resistance to alkaline stress but increased tolerance to oxidation and heavy metal ions, especially Mn2+. Quantitative PCR revealed the upregulation of the DDR2 gene implicated in multiple stress responses and downregulation of PHO84 encoding a phosphate and Mn2+ transporter, which could account for the effects on phosphate uptake and Mn2+-related stress response in the Δvtc4 mutant. Our study indicates that short-chain polyPs, plays an important role in the regulation of stress response in yeast.

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Ultrastructural investigation of acidocalcisomes and ATPase activity in yeast Candida guilliermondii NP‐4 as ‘complementary’ stress‐targets

Cited by 6 publications

References 23 publications

Stress Resistance of Saccharomyces cerevisiae Strains Overexpressing Yeast Polyphosphatases

Stress Resistance of Saccharomyces cerevisiae Strains Overexpressing Yeast Polyphosphatases

VTC4 Polyphosphate Polymerase Knockout Increases Stress Resistance of Saccharomyces cerevisiae Cells

Role of VTC4 in Stress Response and Regulation of Inorganic Polyphosphate Levels in Yeast

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