Ultrastructural identification of mast cells obtained from human bronchial lumens

Ts’ao, Chung-hsin; Patterson, Roy; McKenna, J.; Suszko, Irena M.

doi:10.1016/0091-6749(77)90054-9

Cited by 27 publications

(5 citation statements)

References 13 publications

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“…They concluded that some degranulation occurred in normal lung. Bronchial biopsies and lung biopsies were examined by Ts'ao et al (1977). Alveolar mast cells were different from tissue mast cells with the granules being homogeneous in the former and crystalline in the latter.…”

Section: Normal Mast Cellsmentioning

confidence: 99%

Ultrastructure of nasal mast cells in normal subjects and patients with perennial allergic rhinitis

Drake-Lee¹,

Price²

1991

J. Laryngol. Otol.

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The ultrastructure of mast cells from the nine normal inferior turbinates were compared with those found in eight patients with perennial allergic rhinitis due to house dust mite allergy. Forty-six mast cells from normal patients were found in forty blocks and eighty cells were found in thirty-three blocks in patients with perennial allergy suggesting an increase of mast cell numbers in perennial allergy. There were no basophils outside the blood vessels and whole mast cells were found only in the submucosa. There was no difference in the morphology of cells of different sizes. Mast cells were more degranulated in the allergic mucosa. Degranulation, irrespective of cell size was found at all depths of the mucosa. A review of the literature covered the in vivo and in vitro descriptions of the ultrastructural morphology of human mast cells in the respiratory tract.

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Section: Normal Mast Cellsmentioning

confidence: 99%

Ultrastructure of nasal mast cells in normal subjects and patients with perennial allergic rhinitis

Drake-Lee¹,

Price²

1991

J. Laryngol. Otol.

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“…Cells with a clear basophiloid morphology as defined by Hastie et al [23] were rare, though it is difficult to distinguish basophils and mast cells under the light microscope. However, electron microscope studies have failed to reveal the presence of basophils in BAL fluid [6,24], and for these reasons no attempt was made to count basophils separately from mast cells.…”

Section: Mast Cell Fixationmentioning

confidence: 99%

Histochemical Heterogeneity of Human Mast Cells: Disease-Related Differences in Mast Cell Subsets Recovered by Bronchoalveolar Lavage

Walls

Roberts

Godfrey

et al. 1990

Int Arch Allergy Immunol

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Fixation and staining characteristics were studied for mast cells recovered by bronchoalveolar lavage from 67 patients being investigated for lung disease. The number of toluidine blue stained mast cells in formaldehyde-fixed cytocentrifuge preparations was consistently less than in specimens fixed in Carnoy’s solution, though the counts were highly dependent on the period of fixation or staining. The cellular histamine content closely correlated with total mast cell numbers in bronchoalveolar lavage fluid, but was not related to the relative proportions of mast cells which were sensitive or resistant to formaldehyde fixation when using a standard protocol. Compared with normal subjects, the numbers of formaldehyde-sensitive mast cells were significantly elevated in patients with bronchial carcinoma, sarcoidosis, extrinsic allergic alveolitis, cryptogenic fibrosing alveolitis, and mycobacterial infection and were particularly high in the cases of interstitial lung disease. An even greater increase in numbers of formaldehyde-resistant mast cells was observed in the patients with sarcoidosis and extrinsic allergic alveolitis. The associations of these mast cell subsets with disease may reflect relationships between the expansion of the formaldehyde-sensitive population and lymphocyte infiltration and between proliferation of formaldehyde-resistant mast cells and tissue fibrosis.

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“…Although the mast cells could be identified with ease by routine microscopy, their exact classification was uncertain until appropriate electronmicroscopic studies were performed. These studies defined the bronchial lumen cells from humans as mast cells (4). The presence of living mast cells in the bronchi provides a source of respiratory mast cells for study which can be obtained by noninvasive techniques.…”

Section: Introductionmentioning

confidence: 99%

The in vivo transfer of antigen-induced airway reactions by bronchial lumen cells.

Patterson¹,

Suszko²,

Harris³

1978

J. Clin. Invest.

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Ultrastructural identification of mast cells obtained from human bronchial lumens

Cited by 27 publications

References 13 publications

Ultrastructure of nasal mast cells in normal subjects and patients with perennial allergic rhinitis

Ultrastructure of nasal mast cells in normal subjects and patients with perennial allergic rhinitis

Histochemical Heterogeneity of Human Mast Cells: Disease-Related Differences in Mast Cell Subsets Recovered by Bronchoalveolar Lavage

The in vivo transfer of antigen-induced airway reactions by bronchial lumen cells.

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