Ultrastructural features of collagen in thyroid carcinoma tissue observed by polarization second harmonic generation microscopy

Tokarz, Danielle; Cisek, Richard; Golaraei, Ahmad; Sylvia, L.; Barzda, Virginijus; Wilson, Brian C.

doi:10.1364/boe.6.003475

Cited by 58 publications

(47 citation statements)

References 20 publications

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“…The ratio of the susceptibility tensor elements, including

R = χ_{zzz}^{{((), 2)}^{'}} / χ_{zxx}^{{((), 2)}^{'}}

, was extracted for each pixel of the image. In addition, the average fibril orientation in the image plane within each pixel and the degree of linear polarization was deduced .…”

Section: Introductionmentioning

confidence: 99%

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Golaraei

Mirsanaye

et al. 2018

Journal of Biophotonics

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Polarization‐dependent second‐harmonic generation (P‐SHG) microscopy is used to characterize molecular nonlinear optical properties of collagen and determine a three‐dimensional (3D) orientation map of collagen fibers within a pig tendon. C6 symmetry is used to determine the nonlinear susceptibility tensor components ratios in the molecular frame of reference χzzz2/χzxx2 and χxyz2/χzxx2, where the latter is a newly extracted parameter from the P‐SHG images and is related to the chiral structure of collagen. The χxyz2/χzxx2 is observed for collagen fibers tilted out of the image plane, and can have positive or negative values, revealing the relative polarity of collagen fibers within the tissue. The P‐SHG imaging was performed using a linear polarization‐in polarization‐out (PIPO) method on thin sections of pig tendon cut at different angles. The nonlinear chiral properties of collagen can be used to construct the 3D organization of collagen in the tissue and determine the orientation‐independent molecular susceptibility ratios of collagen fibers in the molecular frame of reference.

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“…The ratio of the susceptibility tensor elements, including

R = χ_{zzz}^{{((), 2)}^{'}} / χ_{zxx}^{{((), 2)}^{'}}

, was extracted for each pixel of the image. In addition, the average fibril orientation in the image plane within each pixel and the degree of linear polarization was deduced .…”

Section: Introductionmentioning

confidence: 99%

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Golaraei

Mirsanaye

et al. 2018

Journal of Biophotonics

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“…Based on the mechanisms above discussed, imaging a specific area of a collagenous tissue with PSHG under different polarization configurations can result in a set of SHG images, each of these having distinct characteristics in terms of content and appearance. The information available in such PSHG sets can be exploited to extract quantitative measures with specific physiological or pathological relevance 13 , 14 , 16 – 19 . In the same time, PSHG data sets can be also subjected to qualitative analyses performed by either human or automated experts.…”

Section: Introductionmentioning

confidence: 99%

A Study on Image Quality in Polarization-Resolved Second Harmonic Generation Microscopy

Stanciu

Ávila

Hristu

et al. 2017

Sci Rep

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Second harmonic generation (SHG) microscopy represents a very powerful tool for tissue characterization. Polarization-resolved SHG (PSHG) microscopy extends the potential of SHG, by exploiting the dependence of SHG signals on the polarization state of the excitation beam. Among others, this dependence translates to the fact that SHG images collected under different polarization configurations exhibit distinct characteristics in terms of content and appearance. These characteristics hold deep implications over image quality, as perceived by human observers or by image analysis methods custom designed to automatically extract a quality factor from digital images. Our work addresses this subject, by investigating how basic image properties and the outputs of no-reference image quality assessment methods correlate to human expert opinion in the case of PSHG micrographs. Our evaluation framework is based on SHG imaging of collagen-based ocular tissues under different linear and elliptical polarization states of the incident light.Second Harmonic Generation (SHG) microscopy is regarded nowadays as a very useful and powerful tool for characterizing biological tissues 1,2 . Its potential originates from the ability to image in a label-free manner non-centrosymmetric structures, which exhibit a non-vanishing second-order susceptibility tensor χ (2) . Under the influence of an external electric field, such structures generate a nonlinear optical signal at exactly half the wavelength of the excitation source. This emission can be easily isolated from the excitation wavelength or from associated fluorescence signals by using spectral filters. Most SHG applications that focus on physiological assessment and disease diagnostics rely on imaging type-I collagen 3-6 , the most abundant protein in the human body and the main structural protein in the extracellular matrix of animal tissues. The investigation of collagen distribution in tissues with SHG enables a precise and non-invasive assessment of extracellular matrix modifications, which represent a hallmark for a wide range of pathologies, including cancer 7,8 . Polarization-resolved SHG (PSHG) microscopy extends the potential of SHG, by exploiting the fact that this nonlinear signal is sensitive to the polarization state of the excitation beam 9 . In the case of collagen-based tissues, the SHG emission depends on the alignment between the collagen fibers/fibrils and the polarization of the excitation light [10][11][12] . The additional dimension available in PSHG data sets can be used to analyze the optical anisotropy and hence to better probe the molecular organization and the external arrangement of collagen-based structures [10][11][12][13][14][15][16] . With the hierarchical organization of collagen being closely intertwined with the biophysical, mechanical and hence functional properties of most tissues, PSHG's potential can be steered towards finding answers to important questions over diseases genesis, progression and treatment. Moreover, PSHG can potentially be used ...

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“…The R ‐ratio has been previously used to characterize different biomaterials such as tendon , bone , muscle , and starch . The R ‐ratio was further used to differentiate between normal and cancerous tissues in various organs such as lung , breast , thyroid , and pancreas . The R ratio measurement can be used as a complementary method along with other histopathology techniques for tissue characterization and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Dual‐ and single‐shot susceptibility ratio measurements with circular polarizations in second‐harmonic generation microscopy

Golaraei

Kontenis

Karunendiran

et al. 2020

Journal of Biophotonics

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Ultrastructural features of collagen in thyroid carcinoma tissue observed by polarization second harmonic generation microscopy

Cited by 58 publications

References 20 publications

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

Collagen chirality and three‐dimensional orientation studied with polarimetric second‐harmonic generation microscopy

A Study on Image Quality in Polarization-Resolved Second Harmonic Generation Microscopy

Dual‐ and single‐shot susceptibility ratio measurements with circular polarizations in second‐harmonic generation microscopy

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